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Citrinin Is a Potential Quorum Sensing Inhibitor against Pseudomonas aeruginosa

Pseudomonas aeruginosa is an opportunistic pathogen that infects patients by regulating virulence factors and biofilms through a quorum sensing (QS) system to protect itself from antibiotics and environmental stress. Therefore, the development of quorum sensing inhibitors (QSIs) is expected to becom...

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Autores principales: Ji, Hongrui, Zhao, Lu, Lv, Kaiwen, Zhang, Yuzhu, Gao, Haibo, Gong, Qianhong, Yu, Wengong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221562/
https://www.ncbi.nlm.nih.gov/pubmed/37233490
http://dx.doi.org/10.3390/md21050296
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author Ji, Hongrui
Zhao, Lu
Lv, Kaiwen
Zhang, Yuzhu
Gao, Haibo
Gong, Qianhong
Yu, Wengong
author_facet Ji, Hongrui
Zhao, Lu
Lv, Kaiwen
Zhang, Yuzhu
Gao, Haibo
Gong, Qianhong
Yu, Wengong
author_sort Ji, Hongrui
collection PubMed
description Pseudomonas aeruginosa is an opportunistic pathogen that infects patients by regulating virulence factors and biofilms through a quorum sensing (QS) system to protect itself from antibiotics and environmental stress. Therefore, the development of quorum sensing inhibitors (QSIs) is expected to become a new strategy for studying drug resistance to P. aeruginosa infections. Marine fungi are valuable resources for screening QSIs. A marine fungus, Penicillium sp. JH1, with anti-QS activity was isolated from the offshore waters of Qingdao (China), and citrinin, a novel QSI, was purified from secondary metabolites of this fungus. Citrinin could significantly inhibit the production of violacein in Chromobacterium violaceum CV12472 and the production of three virulence factors (elastase, rhamnolipid and pyocyanin) in P. aeruginosa PAO1. It could also inhibit the biofilm formation and motility of PAO1. In addition, citrinin downregulated the transcript levels of nine genes (lasI, rhlI, pqsA, lasR, rhlR, pqsR, lasB, rhlA and phzH) associated with QS. Molecular docking results showed that citrinin bound to PqsR and LasR with better affinity than the natural ligands. This study laid a foundation for the further study of the structure optimization and structure–activity relationship of citrinin.
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spelling pubmed-102215622023-05-28 Citrinin Is a Potential Quorum Sensing Inhibitor against Pseudomonas aeruginosa Ji, Hongrui Zhao, Lu Lv, Kaiwen Zhang, Yuzhu Gao, Haibo Gong, Qianhong Yu, Wengong Mar Drugs Article Pseudomonas aeruginosa is an opportunistic pathogen that infects patients by regulating virulence factors and biofilms through a quorum sensing (QS) system to protect itself from antibiotics and environmental stress. Therefore, the development of quorum sensing inhibitors (QSIs) is expected to become a new strategy for studying drug resistance to P. aeruginosa infections. Marine fungi are valuable resources for screening QSIs. A marine fungus, Penicillium sp. JH1, with anti-QS activity was isolated from the offshore waters of Qingdao (China), and citrinin, a novel QSI, was purified from secondary metabolites of this fungus. Citrinin could significantly inhibit the production of violacein in Chromobacterium violaceum CV12472 and the production of three virulence factors (elastase, rhamnolipid and pyocyanin) in P. aeruginosa PAO1. It could also inhibit the biofilm formation and motility of PAO1. In addition, citrinin downregulated the transcript levels of nine genes (lasI, rhlI, pqsA, lasR, rhlR, pqsR, lasB, rhlA and phzH) associated with QS. Molecular docking results showed that citrinin bound to PqsR and LasR with better affinity than the natural ligands. This study laid a foundation for the further study of the structure optimization and structure–activity relationship of citrinin. MDPI 2023-05-12 /pmc/articles/PMC10221562/ /pubmed/37233490 http://dx.doi.org/10.3390/md21050296 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ji, Hongrui
Zhao, Lu
Lv, Kaiwen
Zhang, Yuzhu
Gao, Haibo
Gong, Qianhong
Yu, Wengong
Citrinin Is a Potential Quorum Sensing Inhibitor against Pseudomonas aeruginosa
title Citrinin Is a Potential Quorum Sensing Inhibitor against Pseudomonas aeruginosa
title_full Citrinin Is a Potential Quorum Sensing Inhibitor against Pseudomonas aeruginosa
title_fullStr Citrinin Is a Potential Quorum Sensing Inhibitor against Pseudomonas aeruginosa
title_full_unstemmed Citrinin Is a Potential Quorum Sensing Inhibitor against Pseudomonas aeruginosa
title_short Citrinin Is a Potential Quorum Sensing Inhibitor against Pseudomonas aeruginosa
title_sort citrinin is a potential quorum sensing inhibitor against pseudomonas aeruginosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221562/
https://www.ncbi.nlm.nih.gov/pubmed/37233490
http://dx.doi.org/10.3390/md21050296
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