Cargando…
Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in Arabidopsis
Plant cell wall biosynthesis is a complex process that requires proteins and enzymes from glycan synthesis to wall assembly. We show that disruption of At3g50120 (DUF247-1), a member of the DUF247 multigene family containing 28 genes in Arabidopsis, results in alterations to the structure and compos...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221614/ https://www.ncbi.nlm.nih.gov/pubmed/37653894 http://dx.doi.org/10.3390/plants12101977 |
_version_ | 1785049498480803840 |
---|---|
author | Wannitikul, Pitchaporn Wattana-Amorn, Pakorn Sathitnaitham, Sukhita Sakulkoo, Jenjira Suttangkakul, Anongpat Wonnapinij, Passorn Bassel, George W. Simister, Rachael Gomez, Leonardo D. Vuttipongchaikij, Supachai |
author_facet | Wannitikul, Pitchaporn Wattana-Amorn, Pakorn Sathitnaitham, Sukhita Sakulkoo, Jenjira Suttangkakul, Anongpat Wonnapinij, Passorn Bassel, George W. Simister, Rachael Gomez, Leonardo D. Vuttipongchaikij, Supachai |
author_sort | Wannitikul, Pitchaporn |
collection | PubMed |
description | Plant cell wall biosynthesis is a complex process that requires proteins and enzymes from glycan synthesis to wall assembly. We show that disruption of At3g50120 (DUF247-1), a member of the DUF247 multigene family containing 28 genes in Arabidopsis, results in alterations to the structure and composition of cell wall polysaccharides and reduced growth and plant size. An ELISA using cell wall antibodies shows that the mutants also exhibit ~50% reductions in xyloglucan (XyG), glucuronoxylan (GX) and heteromannan (HM) epitopes in the NaOH fraction and ~50% increases in homogalacturonan (HG) epitopes in the CDTA fraction. Furthermore, the polymer sizes of XyGs and GXs are reduced with concomitant increases in short-chain polymers, while those of HGs and mHGs are slightly increased. Complementation using 35S:DUF247-1 partially recovers the XyG and HG content, but not those of GX and HM, suggesting that DUF247-1 is more closely associated with XyGs and HGs. DUF247-1 is expressed throughout Arabidopsis, particularly in vascular and developing tissues, and its disruption affects the expression of other gene members, indicating a regulatory control role within the gene family. Our results demonstrate that DUF247-1 is required for normal cell wall composition and structure and Arabidopsis growth. |
format | Online Article Text |
id | pubmed-10221614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102216142023-05-28 Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in Arabidopsis Wannitikul, Pitchaporn Wattana-Amorn, Pakorn Sathitnaitham, Sukhita Sakulkoo, Jenjira Suttangkakul, Anongpat Wonnapinij, Passorn Bassel, George W. Simister, Rachael Gomez, Leonardo D. Vuttipongchaikij, Supachai Plants (Basel) Article Plant cell wall biosynthesis is a complex process that requires proteins and enzymes from glycan synthesis to wall assembly. We show that disruption of At3g50120 (DUF247-1), a member of the DUF247 multigene family containing 28 genes in Arabidopsis, results in alterations to the structure and composition of cell wall polysaccharides and reduced growth and plant size. An ELISA using cell wall antibodies shows that the mutants also exhibit ~50% reductions in xyloglucan (XyG), glucuronoxylan (GX) and heteromannan (HM) epitopes in the NaOH fraction and ~50% increases in homogalacturonan (HG) epitopes in the CDTA fraction. Furthermore, the polymer sizes of XyGs and GXs are reduced with concomitant increases in short-chain polymers, while those of HGs and mHGs are slightly increased. Complementation using 35S:DUF247-1 partially recovers the XyG and HG content, but not those of GX and HM, suggesting that DUF247-1 is more closely associated with XyGs and HGs. DUF247-1 is expressed throughout Arabidopsis, particularly in vascular and developing tissues, and its disruption affects the expression of other gene members, indicating a regulatory control role within the gene family. Our results demonstrate that DUF247-1 is required for normal cell wall composition and structure and Arabidopsis growth. MDPI 2023-05-15 /pmc/articles/PMC10221614/ /pubmed/37653894 http://dx.doi.org/10.3390/plants12101977 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wannitikul, Pitchaporn Wattana-Amorn, Pakorn Sathitnaitham, Sukhita Sakulkoo, Jenjira Suttangkakul, Anongpat Wonnapinij, Passorn Bassel, George W. Simister, Rachael Gomez, Leonardo D. Vuttipongchaikij, Supachai Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in Arabidopsis |
title | Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in Arabidopsis |
title_full | Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in Arabidopsis |
title_fullStr | Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in Arabidopsis |
title_full_unstemmed | Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in Arabidopsis |
title_short | Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in Arabidopsis |
title_sort | disruption of a duf247 containing protein alters cell wall polysaccharides and reduces growth in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221614/ https://www.ncbi.nlm.nih.gov/pubmed/37653894 http://dx.doi.org/10.3390/plants12101977 |
work_keys_str_mv | AT wannitikulpitchaporn disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis AT wattanaamornpakorn disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis AT sathitnaithamsukhita disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis AT sakulkoojenjira disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis AT suttangkakulanongpat disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis AT wonnapinijpassorn disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis AT basselgeorgew disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis AT simisterrachael disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis AT gomezleonardod disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis AT vuttipongchaikijsupachai disruptionofaduf247containingproteinalterscellwallpolysaccharidesandreducesgrowthinarabidopsis |