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Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis

American tegumentary leishmaniasis, a zoonotic disease caused by the Leishmania genus, poses significant challenges in treatment, including administration difficulty, low efficacy, and parasite resistance. Novel compounds or associations offer alternative therapies, and natural products such as oreg...

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Autores principales: Alves, Alex Barbosa, da Silva Bortoleti, Bruna Taciane, Tomiotto-Pellissier, Fernanda, Ganaza, Ana Flávia Marques, Gonçalves, Manoela Daiele, Carloto, Amanda Cristina Machado, Rodrigues, Ana Carolina Jacob, Silva, Taylon Felipe, Nakazato, Gerson, Kobayashi, Renata Katsuko Takayama, Lazarin-Bidóia, Danielle, Miranda-Sapla, Milena Menegazzo, Costa, Idessania Nazareth, Pavanelli, Wander Rogério, Conchon-Costa, Ivete
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221720/
https://www.ncbi.nlm.nih.gov/pubmed/37242330
http://dx.doi.org/10.3390/pathogens12050660
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author Alves, Alex Barbosa
da Silva Bortoleti, Bruna Taciane
Tomiotto-Pellissier, Fernanda
Ganaza, Ana Flávia Marques
Gonçalves, Manoela Daiele
Carloto, Amanda Cristina Machado
Rodrigues, Ana Carolina Jacob
Silva, Taylon Felipe
Nakazato, Gerson
Kobayashi, Renata Katsuko Takayama
Lazarin-Bidóia, Danielle
Miranda-Sapla, Milena Menegazzo
Costa, Idessania Nazareth
Pavanelli, Wander Rogério
Conchon-Costa, Ivete
author_facet Alves, Alex Barbosa
da Silva Bortoleti, Bruna Taciane
Tomiotto-Pellissier, Fernanda
Ganaza, Ana Flávia Marques
Gonçalves, Manoela Daiele
Carloto, Amanda Cristina Machado
Rodrigues, Ana Carolina Jacob
Silva, Taylon Felipe
Nakazato, Gerson
Kobayashi, Renata Katsuko Takayama
Lazarin-Bidóia, Danielle
Miranda-Sapla, Milena Menegazzo
Costa, Idessania Nazareth
Pavanelli, Wander Rogério
Conchon-Costa, Ivete
author_sort Alves, Alex Barbosa
collection PubMed
description American tegumentary leishmaniasis, a zoonotic disease caused by the Leishmania genus, poses significant challenges in treatment, including administration difficulty, low efficacy, and parasite resistance. Novel compounds or associations offer alternative therapies, and natural products such as oregano essential oil (OEO), extracted from Origanum vulgare, have been extensively researched due to biological effects, including antibacterial, antifungal, and antiparasitic properties. Silver nanoparticles (AgNp), a nanomaterial with compelling antimicrobial and antiparasitic activity, have been shown to exhibit potent leishmanicidal properties. We evaluated the in vitro effect of OEO and AgNp-Bio association on L. amazonensis and the death mechanisms of the parasite involved. Our results demonstrated a synergistic antileishmanial effect of OEO + AgNp on promastigote forms and L. amazonensis-infected macrophages, which induced morphological and ultrastructural changes in promastigotes. Subsequently, we investigated the mechanisms underlying parasite death and showed an increase in NO, ROS, mitochondrial depolarization, accumulation of lipid-storage bodies, autophagic vacuoles, phosphatidylserine exposure, and damage to the plasma membrane. Moreover, the association resulted in a reduction in the percentage of infected cells and the number of amastigotes per macrophage. In conclusion, our findings establish that OEO + AgNp elicits a late apoptosis-like mechanism to combat promastigote forms and promotes ROS and NO production in infected macrophages to target intracellular amastigote forms.
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spelling pubmed-102217202023-05-28 Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis Alves, Alex Barbosa da Silva Bortoleti, Bruna Taciane Tomiotto-Pellissier, Fernanda Ganaza, Ana Flávia Marques Gonçalves, Manoela Daiele Carloto, Amanda Cristina Machado Rodrigues, Ana Carolina Jacob Silva, Taylon Felipe Nakazato, Gerson Kobayashi, Renata Katsuko Takayama Lazarin-Bidóia, Danielle Miranda-Sapla, Milena Menegazzo Costa, Idessania Nazareth Pavanelli, Wander Rogério Conchon-Costa, Ivete Pathogens Article American tegumentary leishmaniasis, a zoonotic disease caused by the Leishmania genus, poses significant challenges in treatment, including administration difficulty, low efficacy, and parasite resistance. Novel compounds or associations offer alternative therapies, and natural products such as oregano essential oil (OEO), extracted from Origanum vulgare, have been extensively researched due to biological effects, including antibacterial, antifungal, and antiparasitic properties. Silver nanoparticles (AgNp), a nanomaterial with compelling antimicrobial and antiparasitic activity, have been shown to exhibit potent leishmanicidal properties. We evaluated the in vitro effect of OEO and AgNp-Bio association on L. amazonensis and the death mechanisms of the parasite involved. Our results demonstrated a synergistic antileishmanial effect of OEO + AgNp on promastigote forms and L. amazonensis-infected macrophages, which induced morphological and ultrastructural changes in promastigotes. Subsequently, we investigated the mechanisms underlying parasite death and showed an increase in NO, ROS, mitochondrial depolarization, accumulation of lipid-storage bodies, autophagic vacuoles, phosphatidylserine exposure, and damage to the plasma membrane. Moreover, the association resulted in a reduction in the percentage of infected cells and the number of amastigotes per macrophage. In conclusion, our findings establish that OEO + AgNp elicits a late apoptosis-like mechanism to combat promastigote forms and promotes ROS and NO production in infected macrophages to target intracellular amastigote forms. MDPI 2023-04-29 /pmc/articles/PMC10221720/ /pubmed/37242330 http://dx.doi.org/10.3390/pathogens12050660 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alves, Alex Barbosa
da Silva Bortoleti, Bruna Taciane
Tomiotto-Pellissier, Fernanda
Ganaza, Ana Flávia Marques
Gonçalves, Manoela Daiele
Carloto, Amanda Cristina Machado
Rodrigues, Ana Carolina Jacob
Silva, Taylon Felipe
Nakazato, Gerson
Kobayashi, Renata Katsuko Takayama
Lazarin-Bidóia, Danielle
Miranda-Sapla, Milena Menegazzo
Costa, Idessania Nazareth
Pavanelli, Wander Rogério
Conchon-Costa, Ivete
Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis
title Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis
title_full Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis
title_fullStr Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis
title_full_unstemmed Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis
title_short Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis
title_sort synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on leishmania amazonensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221720/
https://www.ncbi.nlm.nih.gov/pubmed/37242330
http://dx.doi.org/10.3390/pathogens12050660
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