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Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis
American tegumentary leishmaniasis, a zoonotic disease caused by the Leishmania genus, poses significant challenges in treatment, including administration difficulty, low efficacy, and parasite resistance. Novel compounds or associations offer alternative therapies, and natural products such as oreg...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221720/ https://www.ncbi.nlm.nih.gov/pubmed/37242330 http://dx.doi.org/10.3390/pathogens12050660 |
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author | Alves, Alex Barbosa da Silva Bortoleti, Bruna Taciane Tomiotto-Pellissier, Fernanda Ganaza, Ana Flávia Marques Gonçalves, Manoela Daiele Carloto, Amanda Cristina Machado Rodrigues, Ana Carolina Jacob Silva, Taylon Felipe Nakazato, Gerson Kobayashi, Renata Katsuko Takayama Lazarin-Bidóia, Danielle Miranda-Sapla, Milena Menegazzo Costa, Idessania Nazareth Pavanelli, Wander Rogério Conchon-Costa, Ivete |
author_facet | Alves, Alex Barbosa da Silva Bortoleti, Bruna Taciane Tomiotto-Pellissier, Fernanda Ganaza, Ana Flávia Marques Gonçalves, Manoela Daiele Carloto, Amanda Cristina Machado Rodrigues, Ana Carolina Jacob Silva, Taylon Felipe Nakazato, Gerson Kobayashi, Renata Katsuko Takayama Lazarin-Bidóia, Danielle Miranda-Sapla, Milena Menegazzo Costa, Idessania Nazareth Pavanelli, Wander Rogério Conchon-Costa, Ivete |
author_sort | Alves, Alex Barbosa |
collection | PubMed |
description | American tegumentary leishmaniasis, a zoonotic disease caused by the Leishmania genus, poses significant challenges in treatment, including administration difficulty, low efficacy, and parasite resistance. Novel compounds or associations offer alternative therapies, and natural products such as oregano essential oil (OEO), extracted from Origanum vulgare, have been extensively researched due to biological effects, including antibacterial, antifungal, and antiparasitic properties. Silver nanoparticles (AgNp), a nanomaterial with compelling antimicrobial and antiparasitic activity, have been shown to exhibit potent leishmanicidal properties. We evaluated the in vitro effect of OEO and AgNp-Bio association on L. amazonensis and the death mechanisms of the parasite involved. Our results demonstrated a synergistic antileishmanial effect of OEO + AgNp on promastigote forms and L. amazonensis-infected macrophages, which induced morphological and ultrastructural changes in promastigotes. Subsequently, we investigated the mechanisms underlying parasite death and showed an increase in NO, ROS, mitochondrial depolarization, accumulation of lipid-storage bodies, autophagic vacuoles, phosphatidylserine exposure, and damage to the plasma membrane. Moreover, the association resulted in a reduction in the percentage of infected cells and the number of amastigotes per macrophage. In conclusion, our findings establish that OEO + AgNp elicits a late apoptosis-like mechanism to combat promastigote forms and promotes ROS and NO production in infected macrophages to target intracellular amastigote forms. |
format | Online Article Text |
id | pubmed-10221720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102217202023-05-28 Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis Alves, Alex Barbosa da Silva Bortoleti, Bruna Taciane Tomiotto-Pellissier, Fernanda Ganaza, Ana Flávia Marques Gonçalves, Manoela Daiele Carloto, Amanda Cristina Machado Rodrigues, Ana Carolina Jacob Silva, Taylon Felipe Nakazato, Gerson Kobayashi, Renata Katsuko Takayama Lazarin-Bidóia, Danielle Miranda-Sapla, Milena Menegazzo Costa, Idessania Nazareth Pavanelli, Wander Rogério Conchon-Costa, Ivete Pathogens Article American tegumentary leishmaniasis, a zoonotic disease caused by the Leishmania genus, poses significant challenges in treatment, including administration difficulty, low efficacy, and parasite resistance. Novel compounds or associations offer alternative therapies, and natural products such as oregano essential oil (OEO), extracted from Origanum vulgare, have been extensively researched due to biological effects, including antibacterial, antifungal, and antiparasitic properties. Silver nanoparticles (AgNp), a nanomaterial with compelling antimicrobial and antiparasitic activity, have been shown to exhibit potent leishmanicidal properties. We evaluated the in vitro effect of OEO and AgNp-Bio association on L. amazonensis and the death mechanisms of the parasite involved. Our results demonstrated a synergistic antileishmanial effect of OEO + AgNp on promastigote forms and L. amazonensis-infected macrophages, which induced morphological and ultrastructural changes in promastigotes. Subsequently, we investigated the mechanisms underlying parasite death and showed an increase in NO, ROS, mitochondrial depolarization, accumulation of lipid-storage bodies, autophagic vacuoles, phosphatidylserine exposure, and damage to the plasma membrane. Moreover, the association resulted in a reduction in the percentage of infected cells and the number of amastigotes per macrophage. In conclusion, our findings establish that OEO + AgNp elicits a late apoptosis-like mechanism to combat promastigote forms and promotes ROS and NO production in infected macrophages to target intracellular amastigote forms. MDPI 2023-04-29 /pmc/articles/PMC10221720/ /pubmed/37242330 http://dx.doi.org/10.3390/pathogens12050660 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alves, Alex Barbosa da Silva Bortoleti, Bruna Taciane Tomiotto-Pellissier, Fernanda Ganaza, Ana Flávia Marques Gonçalves, Manoela Daiele Carloto, Amanda Cristina Machado Rodrigues, Ana Carolina Jacob Silva, Taylon Felipe Nakazato, Gerson Kobayashi, Renata Katsuko Takayama Lazarin-Bidóia, Danielle Miranda-Sapla, Milena Menegazzo Costa, Idessania Nazareth Pavanelli, Wander Rogério Conchon-Costa, Ivete Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis |
title | Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis |
title_full | Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis |
title_fullStr | Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis |
title_full_unstemmed | Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis |
title_short | Synergistic Antileishmanial Effect of Oregano Essential Oil and Silver Nanoparticles: Mechanisms of Action on Leishmania amazonensis |
title_sort | synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on leishmania amazonensis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221720/ https://www.ncbi.nlm.nih.gov/pubmed/37242330 http://dx.doi.org/10.3390/pathogens12050660 |
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