Cargando…

Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis

Polygoni Cuspidati Rhizoma et Radix (PCR), the rhizome and root of Polygonum cuspidatum Sieb. et Zucc., has been used as an herbal medicine for a long time. In this study, the ultrafiltration combined with high performance liquid chromatography (UF-HPLC) method was developed to screen tyrosinase (TY...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Jing, Huang, Qi, He, Zhuobin, Tan, Guoying, Zou, Yuansheng, Xie, Juying, Qian, Zhengming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221946/
https://www.ncbi.nlm.nih.gov/pubmed/37241909
http://dx.doi.org/10.3390/molecules28104170
_version_ 1785049578276388864
author Chen, Jing
Huang, Qi
He, Zhuobin
Tan, Guoying
Zou, Yuansheng
Xie, Juying
Qian, Zhengming
author_facet Chen, Jing
Huang, Qi
He, Zhuobin
Tan, Guoying
Zou, Yuansheng
Xie, Juying
Qian, Zhengming
author_sort Chen, Jing
collection PubMed
description Polygoni Cuspidati Rhizoma et Radix (PCR), the rhizome and root of Polygonum cuspidatum Sieb. et Zucc., has been used as an herbal medicine for a long time. In this study, the ultrafiltration combined with high performance liquid chromatography (UF-HPLC) method was developed to screen tyrosinase (TYR), α-glucosidase (α-GLU), and xanthine oxidase (XOD) inhibitors from PCR. Firstly, the inhibitory activity of 50% methanol PCR extract on TYR, α-GLU, XOD, and acetylcholinesterase (ACHE) was tested. The extract showed a good inhibition on the enzymes, except for ACHE. Therefore, UF-HPLC experiments were carried out to screen TYR, α-GLU, and XOD inhibitors from PCR extract. Seven potential bioactive components were discovered, including methylgallate (1), 1,6-di-O-galloyl-D-glucose (2), polydatin-4′-O-D-glucoside (3), resveratrol-4′-O-D-glucoside (4), polydatin (5), malonyl glucoside resveratrol (6), and resveratrol-5-O-D-glucoside (7). Most of them were found as enzyme inhibitors from PCR for the first time, except polydatin (5), which had been reported as an α-GLUI in PCR in the literature. Finally, molecular docking analysis was applied to validate the interactions of these seven potential active components with the enzymes. Compounds 1–7 were proven as TYR inhibitors, compounds 2, 4–7 were identified as XOD inhibitors, and compounds 4–6 were confirmed as α-GLU inhibitors. In short, the current study provides a good reference for the screening of enzyme inhibitors through UF-HPLC, and provides scientific data for future studies of PCR.
format Online
Article
Text
id pubmed-10221946
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102219462023-05-28 Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis Chen, Jing Huang, Qi He, Zhuobin Tan, Guoying Zou, Yuansheng Xie, Juying Qian, Zhengming Molecules Article Polygoni Cuspidati Rhizoma et Radix (PCR), the rhizome and root of Polygonum cuspidatum Sieb. et Zucc., has been used as an herbal medicine for a long time. In this study, the ultrafiltration combined with high performance liquid chromatography (UF-HPLC) method was developed to screen tyrosinase (TYR), α-glucosidase (α-GLU), and xanthine oxidase (XOD) inhibitors from PCR. Firstly, the inhibitory activity of 50% methanol PCR extract on TYR, α-GLU, XOD, and acetylcholinesterase (ACHE) was tested. The extract showed a good inhibition on the enzymes, except for ACHE. Therefore, UF-HPLC experiments were carried out to screen TYR, α-GLU, and XOD inhibitors from PCR extract. Seven potential bioactive components were discovered, including methylgallate (1), 1,6-di-O-galloyl-D-glucose (2), polydatin-4′-O-D-glucoside (3), resveratrol-4′-O-D-glucoside (4), polydatin (5), malonyl glucoside resveratrol (6), and resveratrol-5-O-D-glucoside (7). Most of them were found as enzyme inhibitors from PCR for the first time, except polydatin (5), which had been reported as an α-GLUI in PCR in the literature. Finally, molecular docking analysis was applied to validate the interactions of these seven potential active components with the enzymes. Compounds 1–7 were proven as TYR inhibitors, compounds 2, 4–7 were identified as XOD inhibitors, and compounds 4–6 were confirmed as α-GLU inhibitors. In short, the current study provides a good reference for the screening of enzyme inhibitors through UF-HPLC, and provides scientific data for future studies of PCR. MDPI 2023-05-18 /pmc/articles/PMC10221946/ /pubmed/37241909 http://dx.doi.org/10.3390/molecules28104170 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Jing
Huang, Qi
He, Zhuobin
Tan, Guoying
Zou, Yuansheng
Xie, Juying
Qian, Zhengming
Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis
title Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis
title_full Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis
title_fullStr Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis
title_full_unstemmed Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis
title_short Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis
title_sort screening of tyrosinase, xanthine oxidase, and α-glucosidase inhibitors from polygoni cuspidati rhizoma et radix by ultrafiltration and hplc analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221946/
https://www.ncbi.nlm.nih.gov/pubmed/37241909
http://dx.doi.org/10.3390/molecules28104170
work_keys_str_mv AT chenjing screeningoftyrosinasexanthineoxidaseandaglucosidaseinhibitorsfrompolygonicuspidatirhizomaetradixbyultrafiltrationandhplcanalysis
AT huangqi screeningoftyrosinasexanthineoxidaseandaglucosidaseinhibitorsfrompolygonicuspidatirhizomaetradixbyultrafiltrationandhplcanalysis
AT hezhuobin screeningoftyrosinasexanthineoxidaseandaglucosidaseinhibitorsfrompolygonicuspidatirhizomaetradixbyultrafiltrationandhplcanalysis
AT tanguoying screeningoftyrosinasexanthineoxidaseandaglucosidaseinhibitorsfrompolygonicuspidatirhizomaetradixbyultrafiltrationandhplcanalysis
AT zouyuansheng screeningoftyrosinasexanthineoxidaseandaglucosidaseinhibitorsfrompolygonicuspidatirhizomaetradixbyultrafiltrationandhplcanalysis
AT xiejuying screeningoftyrosinasexanthineoxidaseandaglucosidaseinhibitorsfrompolygonicuspidatirhizomaetradixbyultrafiltrationandhplcanalysis
AT qianzhengming screeningoftyrosinasexanthineoxidaseandaglucosidaseinhibitorsfrompolygonicuspidatirhizomaetradixbyultrafiltrationandhplcanalysis