Cargando…
Human Ovarian Follicular Fluid Mesenchymal Stem Cells Express Osteogenic Markers When Cultured on Bioglass 58S-Coated Titanium Scaffolds
Recent studies have reported that stem cells (human follicular fluid mesenchymal stem cells or hFF-MSCs) are present in ovarian follicular fluid (hFF) and that they have a proliferative and differentiative potential which is similar to that of MSCs derived from other adult tissue. These mesenchymal...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222050/ https://www.ncbi.nlm.nih.gov/pubmed/37241304 http://dx.doi.org/10.3390/ma16103676 |
_version_ | 1785049603298557952 |
---|---|
author | Riva, Federica Bloise, Nora Omes, Claudia Ceccarelli, Gabriele Fassina, Lorenzo Nappi, Rossella Elena Visai, Livia |
author_facet | Riva, Federica Bloise, Nora Omes, Claudia Ceccarelli, Gabriele Fassina, Lorenzo Nappi, Rossella Elena Visai, Livia |
author_sort | Riva, Federica |
collection | PubMed |
description | Recent studies have reported that stem cells (human follicular fluid mesenchymal stem cells or hFF-MSCs) are present in ovarian follicular fluid (hFF) and that they have a proliferative and differentiative potential which is similar to that of MSCs derived from other adult tissue. These mesenchymal stem cells, isolated from human follicular fluid waste matter discarded after retrieval of oocytes during the IVF process, constitute another, as yet unutilized, source of stem cell materials. There has been little work on the compatibility of these hFF-MSCs with scaffolds useful for bone tissue engineering applications and the aim of this study was to evaluate the osteogenic capacity of hFF-MSCs seeded on bioglass 58S-coated titanium and to provide an assessment of their suitability for bone tissue engineering purposes. Following a chemical and morphological characterization with scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), cell viability, morphology and expression of specific osteogenic markers were examined after 7 and 21 days of culture. The hFF-MSCs seeded on bioglass and cultured with osteogenic factors, when compared with those seeded on tissue culture plate or on uncoated titanium, exhibited enhanced cell viability and osteogenic differentiation, as reflected by increased calcium deposition and increased ALP activity with expression and production of bone-related proteins. Taken together, these results demonstrate that MSCs from human follicular fluid waste materials can be easily cultured in titanium scaffolds coated with bioglass, having osteoinductive properties. This process has significant potential for regenerative medicine applications and indicates that hFF-MSCs may be a valid alternative to hBM-MSC cells in experimental models in bone tissue engineering. |
format | Online Article Text |
id | pubmed-10222050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102220502023-05-28 Human Ovarian Follicular Fluid Mesenchymal Stem Cells Express Osteogenic Markers When Cultured on Bioglass 58S-Coated Titanium Scaffolds Riva, Federica Bloise, Nora Omes, Claudia Ceccarelli, Gabriele Fassina, Lorenzo Nappi, Rossella Elena Visai, Livia Materials (Basel) Article Recent studies have reported that stem cells (human follicular fluid mesenchymal stem cells or hFF-MSCs) are present in ovarian follicular fluid (hFF) and that they have a proliferative and differentiative potential which is similar to that of MSCs derived from other adult tissue. These mesenchymal stem cells, isolated from human follicular fluid waste matter discarded after retrieval of oocytes during the IVF process, constitute another, as yet unutilized, source of stem cell materials. There has been little work on the compatibility of these hFF-MSCs with scaffolds useful for bone tissue engineering applications and the aim of this study was to evaluate the osteogenic capacity of hFF-MSCs seeded on bioglass 58S-coated titanium and to provide an assessment of their suitability for bone tissue engineering purposes. Following a chemical and morphological characterization with scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), cell viability, morphology and expression of specific osteogenic markers were examined after 7 and 21 days of culture. The hFF-MSCs seeded on bioglass and cultured with osteogenic factors, when compared with those seeded on tissue culture plate or on uncoated titanium, exhibited enhanced cell viability and osteogenic differentiation, as reflected by increased calcium deposition and increased ALP activity with expression and production of bone-related proteins. Taken together, these results demonstrate that MSCs from human follicular fluid waste materials can be easily cultured in titanium scaffolds coated with bioglass, having osteoinductive properties. This process has significant potential for regenerative medicine applications and indicates that hFF-MSCs may be a valid alternative to hBM-MSC cells in experimental models in bone tissue engineering. MDPI 2023-05-11 /pmc/articles/PMC10222050/ /pubmed/37241304 http://dx.doi.org/10.3390/ma16103676 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Riva, Federica Bloise, Nora Omes, Claudia Ceccarelli, Gabriele Fassina, Lorenzo Nappi, Rossella Elena Visai, Livia Human Ovarian Follicular Fluid Mesenchymal Stem Cells Express Osteogenic Markers When Cultured on Bioglass 58S-Coated Titanium Scaffolds |
title | Human Ovarian Follicular Fluid Mesenchymal Stem Cells Express Osteogenic Markers When Cultured on Bioglass 58S-Coated Titanium Scaffolds |
title_full | Human Ovarian Follicular Fluid Mesenchymal Stem Cells Express Osteogenic Markers When Cultured on Bioglass 58S-Coated Titanium Scaffolds |
title_fullStr | Human Ovarian Follicular Fluid Mesenchymal Stem Cells Express Osteogenic Markers When Cultured on Bioglass 58S-Coated Titanium Scaffolds |
title_full_unstemmed | Human Ovarian Follicular Fluid Mesenchymal Stem Cells Express Osteogenic Markers When Cultured on Bioglass 58S-Coated Titanium Scaffolds |
title_short | Human Ovarian Follicular Fluid Mesenchymal Stem Cells Express Osteogenic Markers When Cultured on Bioglass 58S-Coated Titanium Scaffolds |
title_sort | human ovarian follicular fluid mesenchymal stem cells express osteogenic markers when cultured on bioglass 58s-coated titanium scaffolds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222050/ https://www.ncbi.nlm.nih.gov/pubmed/37241304 http://dx.doi.org/10.3390/ma16103676 |
work_keys_str_mv | AT rivafederica humanovarianfollicularfluidmesenchymalstemcellsexpressosteogenicmarkerswhenculturedonbioglass58scoatedtitaniumscaffolds AT bloisenora humanovarianfollicularfluidmesenchymalstemcellsexpressosteogenicmarkerswhenculturedonbioglass58scoatedtitaniumscaffolds AT omesclaudia humanovarianfollicularfluidmesenchymalstemcellsexpressosteogenicmarkerswhenculturedonbioglass58scoatedtitaniumscaffolds AT ceccarelligabriele humanovarianfollicularfluidmesenchymalstemcellsexpressosteogenicmarkerswhenculturedonbioglass58scoatedtitaniumscaffolds AT fassinalorenzo humanovarianfollicularfluidmesenchymalstemcellsexpressosteogenicmarkerswhenculturedonbioglass58scoatedtitaniumscaffolds AT nappirossellaelena humanovarianfollicularfluidmesenchymalstemcellsexpressosteogenicmarkerswhenculturedonbioglass58scoatedtitaniumscaffolds AT visailivia humanovarianfollicularfluidmesenchymalstemcellsexpressosteogenicmarkerswhenculturedonbioglass58scoatedtitaniumscaffolds |