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Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function

The effect of asymmetric membranes containing lipopolysaccharides (LPS) on the outer membrane protein F (OmpF) reconstitution, channel orientation, and antibiotic permeation across the outer membrane was investigated. After forming an asymmetric planar lipid bilayer composed of LPS on one and phosph...

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Detalles Bibliográficos
Autores principales: Donoghue, Annemarie, Winterhalter, Mathias, Gutsmann, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222080/
https://www.ncbi.nlm.nih.gov/pubmed/37233578
http://dx.doi.org/10.3390/membranes13050517
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author Donoghue, Annemarie
Winterhalter, Mathias
Gutsmann, Thomas
author_facet Donoghue, Annemarie
Winterhalter, Mathias
Gutsmann, Thomas
author_sort Donoghue, Annemarie
collection PubMed
description The effect of asymmetric membranes containing lipopolysaccharides (LPS) on the outer membrane protein F (OmpF) reconstitution, channel orientation, and antibiotic permeation across the outer membrane was investigated. After forming an asymmetric planar lipid bilayer composed of LPS on one and phospholipids on the other side, the membrane channel OmpF was added. The ion current recordings demonstrate that LPS has a strong influence on the OmpF membrane insertion, orientation, and gating. Enrofloxacin was used as an example of an antibiotic interacting with the asymmetric membrane and with OmpF. The enrofloxacin caused the blockage of the ion current through the OmpF, depending on the side of addition, the transmembrane voltage applied, and the composition of the buffer. Furthermore, the enrofloxacin changed the phase behavior of the LPS-containing membranes, demonstrating that its membrane activity influences the function of OmpF and potentially the membrane permeability.
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spelling pubmed-102220802023-05-28 Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function Donoghue, Annemarie Winterhalter, Mathias Gutsmann, Thomas Membranes (Basel) Article The effect of asymmetric membranes containing lipopolysaccharides (LPS) on the outer membrane protein F (OmpF) reconstitution, channel orientation, and antibiotic permeation across the outer membrane was investigated. After forming an asymmetric planar lipid bilayer composed of LPS on one and phospholipids on the other side, the membrane channel OmpF was added. The ion current recordings demonstrate that LPS has a strong influence on the OmpF membrane insertion, orientation, and gating. Enrofloxacin was used as an example of an antibiotic interacting with the asymmetric membrane and with OmpF. The enrofloxacin caused the blockage of the ion current through the OmpF, depending on the side of addition, the transmembrane voltage applied, and the composition of the buffer. Furthermore, the enrofloxacin changed the phase behavior of the LPS-containing membranes, demonstrating that its membrane activity influences the function of OmpF and potentially the membrane permeability. MDPI 2023-05-16 /pmc/articles/PMC10222080/ /pubmed/37233578 http://dx.doi.org/10.3390/membranes13050517 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Donoghue, Annemarie
Winterhalter, Mathias
Gutsmann, Thomas
Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function
title Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function
title_full Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function
title_fullStr Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function
title_full_unstemmed Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function
title_short Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function
title_sort influence of membrane asymmetry on ompf insertion, orientation and function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222080/
https://www.ncbi.nlm.nih.gov/pubmed/37233578
http://dx.doi.org/10.3390/membranes13050517
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