Cargando…

Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides

Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds Sargassum muticum and Cystoseira myriophyl...

Descripción completa

Detalles Bibliográficos
Autores principales: Aitouguinane, Meriem, El Alaoui-Talibi, Zainab, Rchid, Halima, Fendri, Imen, Abdelkafi, Slim, El-Hadj, Mohamed Didi Ould, Boual, Zakaria, Le Cerf, Didier, Rihouey, Christophe, Gardarin, Christine, Dubessay, Pascal, Michaud, Philippe, Pierre, Guillaume, Delattre, Cédric, El Modafar, Cherkaoui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222107/
https://www.ncbi.nlm.nih.gov/pubmed/37233495
http://dx.doi.org/10.3390/md21050301
_version_ 1785049617584357376
author Aitouguinane, Meriem
El Alaoui-Talibi, Zainab
Rchid, Halima
Fendri, Imen
Abdelkafi, Slim
El-Hadj, Mohamed Didi Ould
Boual, Zakaria
Le Cerf, Didier
Rihouey, Christophe
Gardarin, Christine
Dubessay, Pascal
Michaud, Philippe
Pierre, Guillaume
Delattre, Cédric
El Modafar, Cherkaoui
author_facet Aitouguinane, Meriem
El Alaoui-Talibi, Zainab
Rchid, Halima
Fendri, Imen
Abdelkafi, Slim
El-Hadj, Mohamed Didi Ould
Boual, Zakaria
Le Cerf, Didier
Rihouey, Christophe
Gardarin, Christine
Dubessay, Pascal
Michaud, Philippe
Pierre, Guillaume
Delattre, Cédric
El Modafar, Cherkaoui
author_sort Aitouguinane, Meriem
collection PubMed
description Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds Sargassum muticum and Cystoseira myriophylloides, respectively. Low-molecular-weight alginates (OASM and OACM) were obtained after radical hydrolysis of the native alginates. Elicitation was carried out by foliar spraying 20 mL of aqueous solutions (1 g/L) on 45-day-old tomato seedlings. Elicitor capacities were evaluated by monitoring phenylalanine ammonia-lyase (PAL) activity, polyphenols, and lignin production in the roots and leaves after 0, 12, 24, 48, and 72 h of treatment. The molecular weights (M(w)) of the different fractions were 202 kDa for ALSM, 76 kDa for ALCM, 19 kDa for OACM, and 3 kDa for OASM. FTIR analysis revealed that the structures of OACM and OASM did not change after oxidative degradation of the native alginates. These molecules showed their differential capacity to induce natural defenses in tomato seedlings by increasing PAL activity and through the accumulation of polyphenol and lignin content in the leaves and roots. The oxidative alginates (OASM and OACM) exhibited an effective induction of the key enzyme of phenolic metabolism (PAL) compared to the alginate polymers (ALSM and ALCM). These results suggest that low-molecular-weight alginates may be good candidates for stimulating the natural defenses of plants.
format Online
Article
Text
id pubmed-10222107
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102221072023-05-28 Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides Aitouguinane, Meriem El Alaoui-Talibi, Zainab Rchid, Halima Fendri, Imen Abdelkafi, Slim El-Hadj, Mohamed Didi Ould Boual, Zakaria Le Cerf, Didier Rihouey, Christophe Gardarin, Christine Dubessay, Pascal Michaud, Philippe Pierre, Guillaume Delattre, Cédric El Modafar, Cherkaoui Mar Drugs Article Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds Sargassum muticum and Cystoseira myriophylloides, respectively. Low-molecular-weight alginates (OASM and OACM) were obtained after radical hydrolysis of the native alginates. Elicitation was carried out by foliar spraying 20 mL of aqueous solutions (1 g/L) on 45-day-old tomato seedlings. Elicitor capacities were evaluated by monitoring phenylalanine ammonia-lyase (PAL) activity, polyphenols, and lignin production in the roots and leaves after 0, 12, 24, 48, and 72 h of treatment. The molecular weights (M(w)) of the different fractions were 202 kDa for ALSM, 76 kDa for ALCM, 19 kDa for OACM, and 3 kDa for OASM. FTIR analysis revealed that the structures of OACM and OASM did not change after oxidative degradation of the native alginates. These molecules showed their differential capacity to induce natural defenses in tomato seedlings by increasing PAL activity and through the accumulation of polyphenol and lignin content in the leaves and roots. The oxidative alginates (OASM and OACM) exhibited an effective induction of the key enzyme of phenolic metabolism (PAL) compared to the alginate polymers (ALSM and ALCM). These results suggest that low-molecular-weight alginates may be good candidates for stimulating the natural defenses of plants. MDPI 2023-05-16 /pmc/articles/PMC10222107/ /pubmed/37233495 http://dx.doi.org/10.3390/md21050301 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aitouguinane, Meriem
El Alaoui-Talibi, Zainab
Rchid, Halima
Fendri, Imen
Abdelkafi, Slim
El-Hadj, Mohamed Didi Ould
Boual, Zakaria
Le Cerf, Didier
Rihouey, Christophe
Gardarin, Christine
Dubessay, Pascal
Michaud, Philippe
Pierre, Guillaume
Delattre, Cédric
El Modafar, Cherkaoui
Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides
title Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides
title_full Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides
title_fullStr Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides
title_full_unstemmed Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides
title_short Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides
title_sort elicitor activity of low-molecular-weight alginates obtained by oxidative degradation of alginates extracted from sargassum muticum and cystoseira myriophylloides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222107/
https://www.ncbi.nlm.nih.gov/pubmed/37233495
http://dx.doi.org/10.3390/md21050301
work_keys_str_mv AT aitouguinanemeriem elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT elalaouitalibizainab elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT rchidhalima elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT fendriimen elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT abdelkafislim elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT elhadjmohameddidiould elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT boualzakaria elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT lecerfdidier elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT rihoueychristophe elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT gardarinchristine elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT dubessaypascal elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT michaudphilippe elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT pierreguillaume elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT delattrecedric elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides
AT elmodafarcherkaoui elicitoractivityoflowmolecularweightalginatesobtainedbyoxidativedegradationofalginatesextractedfromsargassummuticumandcystoseiramyriophylloides