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Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides
Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds Sargassum muticum and Cystoseira myriophyl...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222107/ https://www.ncbi.nlm.nih.gov/pubmed/37233495 http://dx.doi.org/10.3390/md21050301 |
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author | Aitouguinane, Meriem El Alaoui-Talibi, Zainab Rchid, Halima Fendri, Imen Abdelkafi, Slim El-Hadj, Mohamed Didi Ould Boual, Zakaria Le Cerf, Didier Rihouey, Christophe Gardarin, Christine Dubessay, Pascal Michaud, Philippe Pierre, Guillaume Delattre, Cédric El Modafar, Cherkaoui |
author_facet | Aitouguinane, Meriem El Alaoui-Talibi, Zainab Rchid, Halima Fendri, Imen Abdelkafi, Slim El-Hadj, Mohamed Didi Ould Boual, Zakaria Le Cerf, Didier Rihouey, Christophe Gardarin, Christine Dubessay, Pascal Michaud, Philippe Pierre, Guillaume Delattre, Cédric El Modafar, Cherkaoui |
author_sort | Aitouguinane, Meriem |
collection | PubMed |
description | Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds Sargassum muticum and Cystoseira myriophylloides, respectively. Low-molecular-weight alginates (OASM and OACM) were obtained after radical hydrolysis of the native alginates. Elicitation was carried out by foliar spraying 20 mL of aqueous solutions (1 g/L) on 45-day-old tomato seedlings. Elicitor capacities were evaluated by monitoring phenylalanine ammonia-lyase (PAL) activity, polyphenols, and lignin production in the roots and leaves after 0, 12, 24, 48, and 72 h of treatment. The molecular weights (M(w)) of the different fractions were 202 kDa for ALSM, 76 kDa for ALCM, 19 kDa for OACM, and 3 kDa for OASM. FTIR analysis revealed that the structures of OACM and OASM did not change after oxidative degradation of the native alginates. These molecules showed their differential capacity to induce natural defenses in tomato seedlings by increasing PAL activity and through the accumulation of polyphenol and lignin content in the leaves and roots. The oxidative alginates (OASM and OACM) exhibited an effective induction of the key enzyme of phenolic metabolism (PAL) compared to the alginate polymers (ALSM and ALCM). These results suggest that low-molecular-weight alginates may be good candidates for stimulating the natural defenses of plants. |
format | Online Article Text |
id | pubmed-10222107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102221072023-05-28 Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides Aitouguinane, Meriem El Alaoui-Talibi, Zainab Rchid, Halima Fendri, Imen Abdelkafi, Slim El-Hadj, Mohamed Didi Ould Boual, Zakaria Le Cerf, Didier Rihouey, Christophe Gardarin, Christine Dubessay, Pascal Michaud, Philippe Pierre, Guillaume Delattre, Cédric El Modafar, Cherkaoui Mar Drugs Article Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds Sargassum muticum and Cystoseira myriophylloides, respectively. Low-molecular-weight alginates (OASM and OACM) were obtained after radical hydrolysis of the native alginates. Elicitation was carried out by foliar spraying 20 mL of aqueous solutions (1 g/L) on 45-day-old tomato seedlings. Elicitor capacities were evaluated by monitoring phenylalanine ammonia-lyase (PAL) activity, polyphenols, and lignin production in the roots and leaves after 0, 12, 24, 48, and 72 h of treatment. The molecular weights (M(w)) of the different fractions were 202 kDa for ALSM, 76 kDa for ALCM, 19 kDa for OACM, and 3 kDa for OASM. FTIR analysis revealed that the structures of OACM and OASM did not change after oxidative degradation of the native alginates. These molecules showed their differential capacity to induce natural defenses in tomato seedlings by increasing PAL activity and through the accumulation of polyphenol and lignin content in the leaves and roots. The oxidative alginates (OASM and OACM) exhibited an effective induction of the key enzyme of phenolic metabolism (PAL) compared to the alginate polymers (ALSM and ALCM). These results suggest that low-molecular-weight alginates may be good candidates for stimulating the natural defenses of plants. MDPI 2023-05-16 /pmc/articles/PMC10222107/ /pubmed/37233495 http://dx.doi.org/10.3390/md21050301 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aitouguinane, Meriem El Alaoui-Talibi, Zainab Rchid, Halima Fendri, Imen Abdelkafi, Slim El-Hadj, Mohamed Didi Ould Boual, Zakaria Le Cerf, Didier Rihouey, Christophe Gardarin, Christine Dubessay, Pascal Michaud, Philippe Pierre, Guillaume Delattre, Cédric El Modafar, Cherkaoui Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides |
title | Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides |
title_full | Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides |
title_fullStr | Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides |
title_full_unstemmed | Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides |
title_short | Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides |
title_sort | elicitor activity of low-molecular-weight alginates obtained by oxidative degradation of alginates extracted from sargassum muticum and cystoseira myriophylloides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222107/ https://www.ncbi.nlm.nih.gov/pubmed/37233495 http://dx.doi.org/10.3390/md21050301 |
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