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Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba)

In this study, we discovered a new virus named Quanzhou mulberry virus (QMV), which was identified from the leaves of an ancient mulberry tree. This tree is over 1300 years old and is located at Fujian Kaiyuan Temple, a renowned cultural heritage site in China. We obtained the complete genome sequen...

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Autores principales: Wei, Jia, Chen, Lei, Xu, Zilong, Liu, Peigang, Zhu, Yan, Lin, Tianbao, Yang, Lu, Huang, Yuan, Lv, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222190/
https://www.ncbi.nlm.nih.gov/pubmed/37243217
http://dx.doi.org/10.3390/v15051131
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author Wei, Jia
Chen, Lei
Xu, Zilong
Liu, Peigang
Zhu, Yan
Lin, Tianbao
Yang, Lu
Huang, Yuan
Lv, Zhiqiang
author_facet Wei, Jia
Chen, Lei
Xu, Zilong
Liu, Peigang
Zhu, Yan
Lin, Tianbao
Yang, Lu
Huang, Yuan
Lv, Zhiqiang
author_sort Wei, Jia
collection PubMed
description In this study, we discovered a new virus named Quanzhou mulberry virus (QMV), which was identified from the leaves of an ancient mulberry tree. This tree is over 1300 years old and is located at Fujian Kaiyuan Temple, a renowned cultural heritage site in China. We obtained the complete genome sequence of QMV using RNA sequencing followed by rapid amplification of complementary DNA ends (RACE). The QMV genome is 9256 nucleotides (nt) long and encodes five open reading frames (ORFs). Its virion was made of icosahedral particles. Phylogenetic analysis suggests that it belongs to the unclassified Riboviria. An infectious clone for QMV was generated and agroinfiltrated into Nicotiana benthamiana and mulberry, resulting in no visible disease symptoms. However, systemic movement of the virus was only observed in mulberry seedlings, suggesting that it has a host-specific pattern of movement. Our findings provide a valuable reference for further studies on QMV and related viruses, contributing to the understanding of viral evolution and biodiversity in mulberry.
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spelling pubmed-102221902023-05-28 Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba) Wei, Jia Chen, Lei Xu, Zilong Liu, Peigang Zhu, Yan Lin, Tianbao Yang, Lu Huang, Yuan Lv, Zhiqiang Viruses Article In this study, we discovered a new virus named Quanzhou mulberry virus (QMV), which was identified from the leaves of an ancient mulberry tree. This tree is over 1300 years old and is located at Fujian Kaiyuan Temple, a renowned cultural heritage site in China. We obtained the complete genome sequence of QMV using RNA sequencing followed by rapid amplification of complementary DNA ends (RACE). The QMV genome is 9256 nucleotides (nt) long and encodes five open reading frames (ORFs). Its virion was made of icosahedral particles. Phylogenetic analysis suggests that it belongs to the unclassified Riboviria. An infectious clone for QMV was generated and agroinfiltrated into Nicotiana benthamiana and mulberry, resulting in no visible disease symptoms. However, systemic movement of the virus was only observed in mulberry seedlings, suggesting that it has a host-specific pattern of movement. Our findings provide a valuable reference for further studies on QMV and related viruses, contributing to the understanding of viral evolution and biodiversity in mulberry. MDPI 2023-05-09 /pmc/articles/PMC10222190/ /pubmed/37243217 http://dx.doi.org/10.3390/v15051131 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Jia
Chen, Lei
Xu, Zilong
Liu, Peigang
Zhu, Yan
Lin, Tianbao
Yang, Lu
Huang, Yuan
Lv, Zhiqiang
Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba)
title Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba)
title_full Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba)
title_fullStr Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba)
title_full_unstemmed Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba)
title_short Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba)
title_sort identification and characterization of a novel quanzhou mulberry virus from mulberry (morus alba)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222190/
https://www.ncbi.nlm.nih.gov/pubmed/37243217
http://dx.doi.org/10.3390/v15051131
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