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Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain

Background: Fibromyalgia (FM) is characterized by complex pain symptoms lacking impersonal considerations in diagnosis and treatment evaluation, which often happens in women. Chronic and persistent widespread pain is the key symptom disturbing patients with FM, leading to depression, obesity, and sl...

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Autores principales: Lai, Po-Chih, Yen, Chia-Ming, Lin, Ming-Chia, Chen, Yung-Hsiang, Liao, Hsien-Yin, Huang, Yu-Wei, Lin, Yi-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222451/
https://www.ncbi.nlm.nih.gov/pubmed/37240805
http://dx.doi.org/10.3390/life13051160
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author Lai, Po-Chih
Yen, Chia-Ming
Lin, Ming-Chia
Chen, Yung-Hsiang
Liao, Hsien-Yin
Huang, Yu-Wei
Lin, Yi-Wen
author_facet Lai, Po-Chih
Yen, Chia-Ming
Lin, Ming-Chia
Chen, Yung-Hsiang
Liao, Hsien-Yin
Huang, Yu-Wei
Lin, Yi-Wen
author_sort Lai, Po-Chih
collection PubMed
description Background: Fibromyalgia (FM) is characterized by complex pain symptoms lacking impersonal considerations in diagnosis and treatment evaluation, which often happens in women. Chronic and persistent widespread pain is the key symptom disturbing patients with FM, leading to depression, obesity, and sleep disturbances. Toll-like receptor 4 (TLR4) activation produces a harmful sensory input involved in central pain; this is the focus of this study. Electroacupuncture (EA) has beneficial effects in reducing FM pain, but its connection with TLR4 signaling is still unknown. Methods: Intermittent cold stress significantly induced mechanical and thermal pain. EA, but not sham EA, reliably attenuated mechanical and thermal hyperalgesia. The increased inflammatory mediators in FM mice were reduced in the EA group, but not in the sham group. Results: All TLR4 and related molecule levels increased in the FM mice’s hypothalamus, periaqueductal gray (PAG), and cerebellum. These increases could be attenuated by EA but not sham stimulation. Activation of TLR4 by lipopolysaccharide (LPS) significantly induced FM and can be further reversed by a TLR4 antagonist. Conclusions: These mechanisms provide evidence that the analgesic effect of EA is related to the TLR4 pathway. In addition, we showed that inflammation can activate the TLR4 pathway and provided new possible therapeutic targets for FM pain.
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spelling pubmed-102224512023-05-28 Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain Lai, Po-Chih Yen, Chia-Ming Lin, Ming-Chia Chen, Yung-Hsiang Liao, Hsien-Yin Huang, Yu-Wei Lin, Yi-Wen Life (Basel) Article Background: Fibromyalgia (FM) is characterized by complex pain symptoms lacking impersonal considerations in diagnosis and treatment evaluation, which often happens in women. Chronic and persistent widespread pain is the key symptom disturbing patients with FM, leading to depression, obesity, and sleep disturbances. Toll-like receptor 4 (TLR4) activation produces a harmful sensory input involved in central pain; this is the focus of this study. Electroacupuncture (EA) has beneficial effects in reducing FM pain, but its connection with TLR4 signaling is still unknown. Methods: Intermittent cold stress significantly induced mechanical and thermal pain. EA, but not sham EA, reliably attenuated mechanical and thermal hyperalgesia. The increased inflammatory mediators in FM mice were reduced in the EA group, but not in the sham group. Results: All TLR4 and related molecule levels increased in the FM mice’s hypothalamus, periaqueductal gray (PAG), and cerebellum. These increases could be attenuated by EA but not sham stimulation. Activation of TLR4 by lipopolysaccharide (LPS) significantly induced FM and can be further reversed by a TLR4 antagonist. Conclusions: These mechanisms provide evidence that the analgesic effect of EA is related to the TLR4 pathway. In addition, we showed that inflammation can activate the TLR4 pathway and provided new possible therapeutic targets for FM pain. MDPI 2023-05-11 /pmc/articles/PMC10222451/ /pubmed/37240805 http://dx.doi.org/10.3390/life13051160 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lai, Po-Chih
Yen, Chia-Ming
Lin, Ming-Chia
Chen, Yung-Hsiang
Liao, Hsien-Yin
Huang, Yu-Wei
Lin, Yi-Wen
Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain
title Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain
title_full Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain
title_fullStr Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain
title_full_unstemmed Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain
title_short Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain
title_sort electroacupuncture attenuates fibromyalgia pain via toll-like receptor 4 in the mouse brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222451/
https://www.ncbi.nlm.nih.gov/pubmed/37240805
http://dx.doi.org/10.3390/life13051160
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