Cargando…
Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring
Food safety is recognized as a main requirement for consumers, food industries, and official laboratories. Here, we present the optimization and screening qualitative validation of two multianalyte methods in bovine muscle tissues by ultra-high-performance liquid chromatography coupled to high-resol...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222520/ https://www.ncbi.nlm.nih.gov/pubmed/37241891 http://dx.doi.org/10.3390/molecules28104150 |
_version_ | 1785049718278062080 |
---|---|
author | Paula, Ramon Alves de Oliveira Gondim, Carina de Souza Schmidt, Eduardo Morgado Diniz, Maria Helena Glicério Marcelina Lana, Mary Ane Gonçalves de Oliveira, Leandro Soares |
author_facet | Paula, Ramon Alves de Oliveira Gondim, Carina de Souza Schmidt, Eduardo Morgado Diniz, Maria Helena Glicério Marcelina Lana, Mary Ane Gonçalves de Oliveira, Leandro Soares |
author_sort | Paula, Ramon Alves de Oliveira |
collection | PubMed |
description | Food safety is recognized as a main requirement for consumers, food industries, and official laboratories. Here, we present the optimization and screening qualitative validation of two multianalyte methods in bovine muscle tissues by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry with an Orbitrap-type analyzer, operated with a heated ionization source in positive and negative mode. This aims for not only the simultaneous detection of veterinary drugs regulated in Brazil but also the prospection of antimicrobials not yet monitored. Two different sample preparation procedures were applied: method A—generic solid-liquid extraction with 0.1% formic acid (v/v) in an aqueous solution of EDTA 0.1% (w/v)—acetonitrile-methanol (1:1:1, v/v/v), followed by an additional ultrasound-assisted extraction and method B—QuEChERS. In both procedures, selectivity showed satisfactory conformity. From a detection capability (CCβ) equivalent to ½ the maximum residue limit, >34% of the analyte resulted in a false positive rate of <5%, preponderant by the QuEChERS method, which exhibited a higher yield of the sample. The results showed the potential application of both procedures in the routine analysis of foods by official laboratories, enabling the expansion of this methodological portfolio as well as its analytical scopes, thus optimizing the control of residues of veterinary drugs in the country. |
format | Online Article Text |
id | pubmed-10222520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102225202023-05-28 Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring Paula, Ramon Alves de Oliveira Gondim, Carina de Souza Schmidt, Eduardo Morgado Diniz, Maria Helena Glicério Marcelina Lana, Mary Ane Gonçalves de Oliveira, Leandro Soares Molecules Article Food safety is recognized as a main requirement for consumers, food industries, and official laboratories. Here, we present the optimization and screening qualitative validation of two multianalyte methods in bovine muscle tissues by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry with an Orbitrap-type analyzer, operated with a heated ionization source in positive and negative mode. This aims for not only the simultaneous detection of veterinary drugs regulated in Brazil but also the prospection of antimicrobials not yet monitored. Two different sample preparation procedures were applied: method A—generic solid-liquid extraction with 0.1% formic acid (v/v) in an aqueous solution of EDTA 0.1% (w/v)—acetonitrile-methanol (1:1:1, v/v/v), followed by an additional ultrasound-assisted extraction and method B—QuEChERS. In both procedures, selectivity showed satisfactory conformity. From a detection capability (CCβ) equivalent to ½ the maximum residue limit, >34% of the analyte resulted in a false positive rate of <5%, preponderant by the QuEChERS method, which exhibited a higher yield of the sample. The results showed the potential application of both procedures in the routine analysis of foods by official laboratories, enabling the expansion of this methodological portfolio as well as its analytical scopes, thus optimizing the control of residues of veterinary drugs in the country. MDPI 2023-05-17 /pmc/articles/PMC10222520/ /pubmed/37241891 http://dx.doi.org/10.3390/molecules28104150 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Paula, Ramon Alves de Oliveira Gondim, Carina de Souza Schmidt, Eduardo Morgado Diniz, Maria Helena Glicério Marcelina Lana, Mary Ane Gonçalves de Oliveira, Leandro Soares Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring |
title | Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring |
title_full | Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring |
title_fullStr | Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring |
title_full_unstemmed | Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring |
title_short | Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring |
title_sort | critical evaluation of two qualitative analytical approaches for multiclass determination of veterinary drugs in bovine muscle using uhplc-q-orbitrap: the wind of change in brazilian monitoring |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222520/ https://www.ncbi.nlm.nih.gov/pubmed/37241891 http://dx.doi.org/10.3390/molecules28104150 |
work_keys_str_mv | AT paularamonalvesdeoliveira criticalevaluationoftwoqualitativeanalyticalapproachesformulticlassdeterminationofveterinarydrugsinbovinemuscleusinguhplcqorbitrapthewindofchangeinbrazilianmonitoring AT gondimcarinadesouza criticalevaluationoftwoqualitativeanalyticalapproachesformulticlassdeterminationofveterinarydrugsinbovinemuscleusinguhplcqorbitrapthewindofchangeinbrazilianmonitoring AT schmidteduardomorgado criticalevaluationoftwoqualitativeanalyticalapproachesformulticlassdeterminationofveterinarydrugsinbovinemuscleusinguhplcqorbitrapthewindofchangeinbrazilianmonitoring AT dinizmariahelenagliceriomarcelina criticalevaluationoftwoqualitativeanalyticalapproachesformulticlassdeterminationofveterinarydrugsinbovinemuscleusinguhplcqorbitrapthewindofchangeinbrazilianmonitoring AT lanamaryanegoncalves criticalevaluationoftwoqualitativeanalyticalapproachesformulticlassdeterminationofveterinarydrugsinbovinemuscleusinguhplcqorbitrapthewindofchangeinbrazilianmonitoring AT deoliveiraleandrosoares criticalevaluationoftwoqualitativeanalyticalapproachesformulticlassdeterminationofveterinarydrugsinbovinemuscleusinguhplcqorbitrapthewindofchangeinbrazilianmonitoring |