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Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring

Food safety is recognized as a main requirement for consumers, food industries, and official laboratories. Here, we present the optimization and screening qualitative validation of two multianalyte methods in bovine muscle tissues by ultra-high-performance liquid chromatography coupled to high-resol...

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Autores principales: Paula, Ramon Alves de Oliveira, Gondim, Carina de Souza, Schmidt, Eduardo Morgado, Diniz, Maria Helena Glicério Marcelina, Lana, Mary Ane Gonçalves, de Oliveira, Leandro Soares
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222520/
https://www.ncbi.nlm.nih.gov/pubmed/37241891
http://dx.doi.org/10.3390/molecules28104150
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author Paula, Ramon Alves de Oliveira
Gondim, Carina de Souza
Schmidt, Eduardo Morgado
Diniz, Maria Helena Glicério Marcelina
Lana, Mary Ane Gonçalves
de Oliveira, Leandro Soares
author_facet Paula, Ramon Alves de Oliveira
Gondim, Carina de Souza
Schmidt, Eduardo Morgado
Diniz, Maria Helena Glicério Marcelina
Lana, Mary Ane Gonçalves
de Oliveira, Leandro Soares
author_sort Paula, Ramon Alves de Oliveira
collection PubMed
description Food safety is recognized as a main requirement for consumers, food industries, and official laboratories. Here, we present the optimization and screening qualitative validation of two multianalyte methods in bovine muscle tissues by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry with an Orbitrap-type analyzer, operated with a heated ionization source in positive and negative mode. This aims for not only the simultaneous detection of veterinary drugs regulated in Brazil but also the prospection of antimicrobials not yet monitored. Two different sample preparation procedures were applied: method A—generic solid-liquid extraction with 0.1% formic acid (v/v) in an aqueous solution of EDTA 0.1% (w/v)—acetonitrile-methanol (1:1:1, v/v/v), followed by an additional ultrasound-assisted extraction and method B—QuEChERS. In both procedures, selectivity showed satisfactory conformity. From a detection capability (CCβ) equivalent to ½ the maximum residue limit, >34% of the analyte resulted in a false positive rate of <5%, preponderant by the QuEChERS method, which exhibited a higher yield of the sample. The results showed the potential application of both procedures in the routine analysis of foods by official laboratories, enabling the expansion of this methodological portfolio as well as its analytical scopes, thus optimizing the control of residues of veterinary drugs in the country.
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spelling pubmed-102225202023-05-28 Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring Paula, Ramon Alves de Oliveira Gondim, Carina de Souza Schmidt, Eduardo Morgado Diniz, Maria Helena Glicério Marcelina Lana, Mary Ane Gonçalves de Oliveira, Leandro Soares Molecules Article Food safety is recognized as a main requirement for consumers, food industries, and official laboratories. Here, we present the optimization and screening qualitative validation of two multianalyte methods in bovine muscle tissues by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry with an Orbitrap-type analyzer, operated with a heated ionization source in positive and negative mode. This aims for not only the simultaneous detection of veterinary drugs regulated in Brazil but also the prospection of antimicrobials not yet monitored. Two different sample preparation procedures were applied: method A—generic solid-liquid extraction with 0.1% formic acid (v/v) in an aqueous solution of EDTA 0.1% (w/v)—acetonitrile-methanol (1:1:1, v/v/v), followed by an additional ultrasound-assisted extraction and method B—QuEChERS. In both procedures, selectivity showed satisfactory conformity. From a detection capability (CCβ) equivalent to ½ the maximum residue limit, >34% of the analyte resulted in a false positive rate of <5%, preponderant by the QuEChERS method, which exhibited a higher yield of the sample. The results showed the potential application of both procedures in the routine analysis of foods by official laboratories, enabling the expansion of this methodological portfolio as well as its analytical scopes, thus optimizing the control of residues of veterinary drugs in the country. MDPI 2023-05-17 /pmc/articles/PMC10222520/ /pubmed/37241891 http://dx.doi.org/10.3390/molecules28104150 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paula, Ramon Alves de Oliveira
Gondim, Carina de Souza
Schmidt, Eduardo Morgado
Diniz, Maria Helena Glicério Marcelina
Lana, Mary Ane Gonçalves
de Oliveira, Leandro Soares
Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring
title Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring
title_full Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring
title_fullStr Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring
title_full_unstemmed Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring
title_short Critical Evaluation of Two Qualitative Analytical Approaches for Multiclass Determination of Veterinary Drugs in Bovine Muscle Using UHPLC-Q-Orbitrap: The Wind of Change in Brazilian Monitoring
title_sort critical evaluation of two qualitative analytical approaches for multiclass determination of veterinary drugs in bovine muscle using uhplc-q-orbitrap: the wind of change in brazilian monitoring
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222520/
https://www.ncbi.nlm.nih.gov/pubmed/37241891
http://dx.doi.org/10.3390/molecules28104150
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