Cargando…

Differences in the Urinary Microbiome of Patients with Overactive Bladder Syndrome with and without Detrusor Overactivity on Urodynamic Measurements

Introduction: It has been hypothesized that the urinary microbiome might play an important role in OAB. Studies have been conducted on the association between OAB symptoms and the microbiome, although a possible causality still has to be determined. Material and Methods: In this study, 12 female pat...

Descripción completa

Detalles Bibliográficos
Autores principales: Javan Balegh Marand, Aida, Baars, Cléo, Heesakkers, John, van den Munckhof, Ellen, Ghojazadeh, Morteza, Rahnama’i, Mohammad Sajjad, Janssen, Dick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222553/
https://www.ncbi.nlm.nih.gov/pubmed/37240844
http://dx.doi.org/10.3390/life13051199
_version_ 1785049726610046976
author Javan Balegh Marand, Aida
Baars, Cléo
Heesakkers, John
van den Munckhof, Ellen
Ghojazadeh, Morteza
Rahnama’i, Mohammad Sajjad
Janssen, Dick
author_facet Javan Balegh Marand, Aida
Baars, Cléo
Heesakkers, John
van den Munckhof, Ellen
Ghojazadeh, Morteza
Rahnama’i, Mohammad Sajjad
Janssen, Dick
author_sort Javan Balegh Marand, Aida
collection PubMed
description Introduction: It has been hypothesized that the urinary microbiome might play an important role in OAB. Studies have been conducted on the association between OAB symptoms and the microbiome, although a possible causality still has to be determined. Material and Methods: In this study, 12 female patients, ≥18 years of age, with ‘OAB DO+’ and 9 female patients with ‘OAB DO-’ were included. Patients were excluded if they met one of the following exclusion criteria: bladder tumors and previous bladder operations; sacral neuromodulation; injection of Botox in the bladder; and TOT or TVT operations. Urine samples were collected and stored with patient informed consent and with the approval of the Hospital Ethical Review Board (Arnhem–Nijmegen). All OAB patients underwent urodynamics before collecting urine samples, and the diagnosis of detrusor overactivity was confirmed by two individual urologists. In addition, samples from 12 healthy controls who did not undergo urodynamic evaluation were analyzed. The 16S rRNA V1–V2 region amplification and gel electrophoresis were used to determine the microbiota. Results: 12 of the OAB patients had DO shown on their urodynamic studies; the remaining 9 patients had a normoactive detrusor on their urodynamic measurements. Overall, there were no substantial differences among the demographic characteristics of the subjects. The samples were classified as the following: 180 phyla, 180 classes, 179 orders, 178 families, 175 genera, and 138 species. The least commonly observed phyla were Proteobacteria, with an average presence of 10%, followed by Bacteroidetes with 15%, Actinobacteria with 16%, and Firmicutes with 41%. Most of the sequences could be classified according to the genus level for each sample. Discussion: Significant differences were observed in the urinary microbiome of patients with overactive bladder syndrome who have detrusor overactivity on urodynamics compared to OAB patients without detrusor overactivity and matched controls. OAB patients with detrusor overactivity have a significantly less diverse microbiome and show a higher proportion of Lactobacillus, particularly Lactobacillus iners. The results imply that the urinary microbiome could be involved in the pathogenesis of a specific phenotype of OAB. The urinary microbiome could be a new starting point to study the causes and treatments of OAB.
format Online
Article
Text
id pubmed-10222553
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102225532023-05-28 Differences in the Urinary Microbiome of Patients with Overactive Bladder Syndrome with and without Detrusor Overactivity on Urodynamic Measurements Javan Balegh Marand, Aida Baars, Cléo Heesakkers, John van den Munckhof, Ellen Ghojazadeh, Morteza Rahnama’i, Mohammad Sajjad Janssen, Dick Life (Basel) Article Introduction: It has been hypothesized that the urinary microbiome might play an important role in OAB. Studies have been conducted on the association between OAB symptoms and the microbiome, although a possible causality still has to be determined. Material and Methods: In this study, 12 female patients, ≥18 years of age, with ‘OAB DO+’ and 9 female patients with ‘OAB DO-’ were included. Patients were excluded if they met one of the following exclusion criteria: bladder tumors and previous bladder operations; sacral neuromodulation; injection of Botox in the bladder; and TOT or TVT operations. Urine samples were collected and stored with patient informed consent and with the approval of the Hospital Ethical Review Board (Arnhem–Nijmegen). All OAB patients underwent urodynamics before collecting urine samples, and the diagnosis of detrusor overactivity was confirmed by two individual urologists. In addition, samples from 12 healthy controls who did not undergo urodynamic evaluation were analyzed. The 16S rRNA V1–V2 region amplification and gel electrophoresis were used to determine the microbiota. Results: 12 of the OAB patients had DO shown on their urodynamic studies; the remaining 9 patients had a normoactive detrusor on their urodynamic measurements. Overall, there were no substantial differences among the demographic characteristics of the subjects. The samples were classified as the following: 180 phyla, 180 classes, 179 orders, 178 families, 175 genera, and 138 species. The least commonly observed phyla were Proteobacteria, with an average presence of 10%, followed by Bacteroidetes with 15%, Actinobacteria with 16%, and Firmicutes with 41%. Most of the sequences could be classified according to the genus level for each sample. Discussion: Significant differences were observed in the urinary microbiome of patients with overactive bladder syndrome who have detrusor overactivity on urodynamics compared to OAB patients without detrusor overactivity and matched controls. OAB patients with detrusor overactivity have a significantly less diverse microbiome and show a higher proportion of Lactobacillus, particularly Lactobacillus iners. The results imply that the urinary microbiome could be involved in the pathogenesis of a specific phenotype of OAB. The urinary microbiome could be a new starting point to study the causes and treatments of OAB. MDPI 2023-05-17 /pmc/articles/PMC10222553/ /pubmed/37240844 http://dx.doi.org/10.3390/life13051199 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Javan Balegh Marand, Aida
Baars, Cléo
Heesakkers, John
van den Munckhof, Ellen
Ghojazadeh, Morteza
Rahnama’i, Mohammad Sajjad
Janssen, Dick
Differences in the Urinary Microbiome of Patients with Overactive Bladder Syndrome with and without Detrusor Overactivity on Urodynamic Measurements
title Differences in the Urinary Microbiome of Patients with Overactive Bladder Syndrome with and without Detrusor Overactivity on Urodynamic Measurements
title_full Differences in the Urinary Microbiome of Patients with Overactive Bladder Syndrome with and without Detrusor Overactivity on Urodynamic Measurements
title_fullStr Differences in the Urinary Microbiome of Patients with Overactive Bladder Syndrome with and without Detrusor Overactivity on Urodynamic Measurements
title_full_unstemmed Differences in the Urinary Microbiome of Patients with Overactive Bladder Syndrome with and without Detrusor Overactivity on Urodynamic Measurements
title_short Differences in the Urinary Microbiome of Patients with Overactive Bladder Syndrome with and without Detrusor Overactivity on Urodynamic Measurements
title_sort differences in the urinary microbiome of patients with overactive bladder syndrome with and without detrusor overactivity on urodynamic measurements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222553/
https://www.ncbi.nlm.nih.gov/pubmed/37240844
http://dx.doi.org/10.3390/life13051199
work_keys_str_mv AT javanbaleghmarandaida differencesintheurinarymicrobiomeofpatientswithoveractivebladdersyndromewithandwithoutdetrusoroveractivityonurodynamicmeasurements
AT baarscleo differencesintheurinarymicrobiomeofpatientswithoveractivebladdersyndromewithandwithoutdetrusoroveractivityonurodynamicmeasurements
AT heesakkersjohn differencesintheurinarymicrobiomeofpatientswithoveractivebladdersyndromewithandwithoutdetrusoroveractivityonurodynamicmeasurements
AT vandenmunckhofellen differencesintheurinarymicrobiomeofpatientswithoveractivebladdersyndromewithandwithoutdetrusoroveractivityonurodynamicmeasurements
AT ghojazadehmorteza differencesintheurinarymicrobiomeofpatientswithoveractivebladdersyndromewithandwithoutdetrusoroveractivityonurodynamicmeasurements
AT rahnamaimohammadsajjad differencesintheurinarymicrobiomeofpatientswithoveractivebladdersyndromewithandwithoutdetrusoroveractivityonurodynamicmeasurements
AT janssendick differencesintheurinarymicrobiomeofpatientswithoveractivebladdersyndromewithandwithoutdetrusoroveractivityonurodynamicmeasurements