Cargando…
Collaterally Sensitive β-Lactam Drugs as an Effective Therapy against the Pre-Existing Methicillin Resistant Staphylococcus aureus (MRSA) Biofilms
Methicillin-resistant Staphylococcus aureus (MRSA) is among the leading causes of nosocomial infections and forms biofilms, which are difficult to eradicate because of their increasing resistance to antimicrobial agents. This is especially true for pre-existing biofilms. The current study focused on...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222715/ https://www.ncbi.nlm.nih.gov/pubmed/37242471 http://dx.doi.org/10.3390/ph16050687 |
_version_ | 1785049765761777664 |
---|---|
author | Hashmi, Hamna Batool Farooq, Muhammad Asad Khan, Muhammad Hashim Alshammari, Abdulrahman Aljasham, Alanoud T. Rashid, Sheikh Abdur Khan, Nauman Rahim Hashmi, Irum Batool Badar, Muhammad Mubarak, Mohammad S. |
author_facet | Hashmi, Hamna Batool Farooq, Muhammad Asad Khan, Muhammad Hashim Alshammari, Abdulrahman Aljasham, Alanoud T. Rashid, Sheikh Abdur Khan, Nauman Rahim Hashmi, Irum Batool Badar, Muhammad Mubarak, Mohammad S. |
author_sort | Hashmi, Hamna Batool |
collection | PubMed |
description | Methicillin-resistant Staphylococcus aureus (MRSA) is among the leading causes of nosocomial infections and forms biofilms, which are difficult to eradicate because of their increasing resistance to antimicrobial agents. This is especially true for pre-existing biofilms. The current study focused on evaluating the efficacy of three β-lactam drugs, meropenem, piperacillin, and tazobactam, alone and in combination against the MRSA biofilms. When used individually, none of the drugs exhibited significant antibacterial activity against MRSA in a planktonic state. At the same time, the combination of meropenem, piperacillin, and tazobactam showed a 41.7 and 41.3% reduction in planktonic bacterial cell growth, respectively. These drugs were further assessed for biofilm inhibition and removal. The combination of meropenem, piperacillin, and tazobactam caused 44.3% biofilm inhibition, while the rest of the combinations did not show any significant effects. Results also revealed that piperacillin and tazobactam exhibited the best synergy against the pre-formed biofilm of MRSA, with 46% removal. However, adding meropenem to the piperacillin and tazobactam combination showed a slightly reduced activity towards the pre-formed biofilm of MRSA and removed 38.7% of it. Although the mechanism of synergism is not fully understood, our findings suggest that these three β-lactam drugs can be used in combination as very effective therapeutic agents for the treatment of pre-existing MRSA biofilms. The in vivo experiments on the antibiofilm activity of these drugs will pave the way for applying such synergistic combinations to clinics. |
format | Online Article Text |
id | pubmed-10222715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102227152023-05-28 Collaterally Sensitive β-Lactam Drugs as an Effective Therapy against the Pre-Existing Methicillin Resistant Staphylococcus aureus (MRSA) Biofilms Hashmi, Hamna Batool Farooq, Muhammad Asad Khan, Muhammad Hashim Alshammari, Abdulrahman Aljasham, Alanoud T. Rashid, Sheikh Abdur Khan, Nauman Rahim Hashmi, Irum Batool Badar, Muhammad Mubarak, Mohammad S. Pharmaceuticals (Basel) Article Methicillin-resistant Staphylococcus aureus (MRSA) is among the leading causes of nosocomial infections and forms biofilms, which are difficult to eradicate because of their increasing resistance to antimicrobial agents. This is especially true for pre-existing biofilms. The current study focused on evaluating the efficacy of three β-lactam drugs, meropenem, piperacillin, and tazobactam, alone and in combination against the MRSA biofilms. When used individually, none of the drugs exhibited significant antibacterial activity against MRSA in a planktonic state. At the same time, the combination of meropenem, piperacillin, and tazobactam showed a 41.7 and 41.3% reduction in planktonic bacterial cell growth, respectively. These drugs were further assessed for biofilm inhibition and removal. The combination of meropenem, piperacillin, and tazobactam caused 44.3% biofilm inhibition, while the rest of the combinations did not show any significant effects. Results also revealed that piperacillin and tazobactam exhibited the best synergy against the pre-formed biofilm of MRSA, with 46% removal. However, adding meropenem to the piperacillin and tazobactam combination showed a slightly reduced activity towards the pre-formed biofilm of MRSA and removed 38.7% of it. Although the mechanism of synergism is not fully understood, our findings suggest that these three β-lactam drugs can be used in combination as very effective therapeutic agents for the treatment of pre-existing MRSA biofilms. The in vivo experiments on the antibiofilm activity of these drugs will pave the way for applying such synergistic combinations to clinics. MDPI 2023-05-02 /pmc/articles/PMC10222715/ /pubmed/37242471 http://dx.doi.org/10.3390/ph16050687 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hashmi, Hamna Batool Farooq, Muhammad Asad Khan, Muhammad Hashim Alshammari, Abdulrahman Aljasham, Alanoud T. Rashid, Sheikh Abdur Khan, Nauman Rahim Hashmi, Irum Batool Badar, Muhammad Mubarak, Mohammad S. Collaterally Sensitive β-Lactam Drugs as an Effective Therapy against the Pre-Existing Methicillin Resistant Staphylococcus aureus (MRSA) Biofilms |
title | Collaterally Sensitive β-Lactam Drugs as an Effective Therapy against the Pre-Existing Methicillin Resistant Staphylococcus aureus (MRSA) Biofilms |
title_full | Collaterally Sensitive β-Lactam Drugs as an Effective Therapy against the Pre-Existing Methicillin Resistant Staphylococcus aureus (MRSA) Biofilms |
title_fullStr | Collaterally Sensitive β-Lactam Drugs as an Effective Therapy against the Pre-Existing Methicillin Resistant Staphylococcus aureus (MRSA) Biofilms |
title_full_unstemmed | Collaterally Sensitive β-Lactam Drugs as an Effective Therapy against the Pre-Existing Methicillin Resistant Staphylococcus aureus (MRSA) Biofilms |
title_short | Collaterally Sensitive β-Lactam Drugs as an Effective Therapy against the Pre-Existing Methicillin Resistant Staphylococcus aureus (MRSA) Biofilms |
title_sort | collaterally sensitive β-lactam drugs as an effective therapy against the pre-existing methicillin resistant staphylococcus aureus (mrsa) biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222715/ https://www.ncbi.nlm.nih.gov/pubmed/37242471 http://dx.doi.org/10.3390/ph16050687 |
work_keys_str_mv | AT hashmihamnabatool collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms AT farooqmuhammadasad collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms AT khanmuhammadhashim collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms AT alshammariabdulrahman collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms AT aljashamalanoudt collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms AT rashidsheikhabdur collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms AT khannaumanrahim collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms AT hashmiirumbatool collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms AT badarmuhammad collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms AT mubarakmohammads collaterallysensitiveblactamdrugsasaneffectivetherapyagainstthepreexistingmethicillinresistantstaphylococcusaureusmrsabiofilms |