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A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs

Porcine deltacoronavirus (PDCoV) is an emergent swine coronavirus which infects cells from the small intestine and induces watery diarrhea, vomiting and dehydration, causing mortality in piglets (>40%). The aim of this study was to evaluate the antigenicity and immunogenicity of the recombinant m...

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Autores principales: Castañeda-Montes, Francisco Jesus, Cerriteño-Sánchez, José Luis, Castañeda-Montes, María Azucena, Cuevas-Romero, Julieta Sandra, Mendoza-Elvira, Susana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222885/
https://www.ncbi.nlm.nih.gov/pubmed/37243136
http://dx.doi.org/10.3390/v15051049
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author Castañeda-Montes, Francisco Jesus
Cerriteño-Sánchez, José Luis
Castañeda-Montes, María Azucena
Cuevas-Romero, Julieta Sandra
Mendoza-Elvira, Susana
author_facet Castañeda-Montes, Francisco Jesus
Cerriteño-Sánchez, José Luis
Castañeda-Montes, María Azucena
Cuevas-Romero, Julieta Sandra
Mendoza-Elvira, Susana
author_sort Castañeda-Montes, Francisco Jesus
collection PubMed
description Porcine deltacoronavirus (PDCoV) is an emergent swine coronavirus which infects cells from the small intestine and induces watery diarrhea, vomiting and dehydration, causing mortality in piglets (>40%). The aim of this study was to evaluate the antigenicity and immunogenicity of the recombinant membrane protein (M) of PDCoV (rM-PDCoV), which was developed from a synthetic gene obtained after an in silico analysis with a group of 138 GenBank sequences. A 3D model and phylogenetic analysis confirmed the highly conserved M protein structure. Therefore, the synthetic gene was successfully cloned in a pETSUMO vector and transformed in E. coli BL21 (DE3). The rM-PDCoV was confirmed by SDS-PAGE and Western blot with ~37.7 kDa. The rM-PDCoV immunogenicity was evaluated in immunized (BLAB/c) mice and iELISA. The data showed increased antibodies from 7 days until 28 days (p < 0.001). The rM-PDCoV antigenicity was analyzed using pig sera samples from three states located in “El Bajío” Mexico and positive sera were determined. Our results show that PDCoV has continued circulating on pig farms in Mexico since the first report in 2019; therefore, the impact of PDCoV on the swine industry could be higher than reported in other studies.
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spelling pubmed-102228852023-05-28 A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs Castañeda-Montes, Francisco Jesus Cerriteño-Sánchez, José Luis Castañeda-Montes, María Azucena Cuevas-Romero, Julieta Sandra Mendoza-Elvira, Susana Viruses Article Porcine deltacoronavirus (PDCoV) is an emergent swine coronavirus which infects cells from the small intestine and induces watery diarrhea, vomiting and dehydration, causing mortality in piglets (>40%). The aim of this study was to evaluate the antigenicity and immunogenicity of the recombinant membrane protein (M) of PDCoV (rM-PDCoV), which was developed from a synthetic gene obtained after an in silico analysis with a group of 138 GenBank sequences. A 3D model and phylogenetic analysis confirmed the highly conserved M protein structure. Therefore, the synthetic gene was successfully cloned in a pETSUMO vector and transformed in E. coli BL21 (DE3). The rM-PDCoV was confirmed by SDS-PAGE and Western blot with ~37.7 kDa. The rM-PDCoV immunogenicity was evaluated in immunized (BLAB/c) mice and iELISA. The data showed increased antibodies from 7 days until 28 days (p < 0.001). The rM-PDCoV antigenicity was analyzed using pig sera samples from three states located in “El Bajío” Mexico and positive sera were determined. Our results show that PDCoV has continued circulating on pig farms in Mexico since the first report in 2019; therefore, the impact of PDCoV on the swine industry could be higher than reported in other studies. MDPI 2023-04-25 /pmc/articles/PMC10222885/ /pubmed/37243136 http://dx.doi.org/10.3390/v15051049 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Castañeda-Montes, Francisco Jesus
Cerriteño-Sánchez, José Luis
Castañeda-Montes, María Azucena
Cuevas-Romero, Julieta Sandra
Mendoza-Elvira, Susana
A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs
title A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs
title_full A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs
title_fullStr A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs
title_full_unstemmed A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs
title_short A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs
title_sort candidate antigen of the recombinant membrane protein derived from the porcine deltacoronavirus synthetic gene to detect seropositive pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222885/
https://www.ncbi.nlm.nih.gov/pubmed/37243136
http://dx.doi.org/10.3390/v15051049
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