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Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets

SIMPLE SUMMARY: Based on the fact that Gps are rooted in the upper respiratory tract of pigs, in order to investigate whether secondary infection with HP-PRRSV can exacerbate lung disease and chronic inflammation, our study was designed as follows. Our study randomly divided piglets into four groups...

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Autores principales: Guan, Zhixin, Pang, Linlin, Ouyang, Yan, Jiang, Yifeng, Zhang, Junjie, Qiu, Yafeng, Li, Zongjie, Li, Beibei, Liu, Ke, Shao, Donghua, Ma, Zhiyong, Wei, Jianchao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222957/
https://www.ncbi.nlm.nih.gov/pubmed/37235448
http://dx.doi.org/10.3390/vetsci10050365
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author Guan, Zhixin
Pang, Linlin
Ouyang, Yan
Jiang, Yifeng
Zhang, Junjie
Qiu, Yafeng
Li, Zongjie
Li, Beibei
Liu, Ke
Shao, Donghua
Ma, Zhiyong
Wei, Jianchao
author_facet Guan, Zhixin
Pang, Linlin
Ouyang, Yan
Jiang, Yifeng
Zhang, Junjie
Qiu, Yafeng
Li, Zongjie
Li, Beibei
Liu, Ke
Shao, Donghua
Ma, Zhiyong
Wei, Jianchao
author_sort Guan, Zhixin
collection PubMed
description SIMPLE SUMMARY: Based on the fact that Gps are rooted in the upper respiratory tract of pigs, in order to investigate whether secondary infection with HP-PRRSV can exacerbate lung disease and chronic inflammation, our study was designed as follows. Our study randomly divided piglets into four groups: Gps + HP-PRRSV, Gps, HP-PRRSV, and controls. Piglets in the Gps + HP-PRRSV and Gps groups were infected through the intranasal route with the Gps W2 strain. The Gps + HP-PRRSV and HP-PRRSV groups were challenged with the HP-PRRSV HuN4 strain by intramuscular injection and intranasally at 5 days after the initial exposure to Gps. Alternatively, the control group animals received phosphate-buffered saline (PBS). Then, we observed the influence of HP-PRRSV–Gps coinfection on clinical outcomes, pathogen shedding and loading, cytokine production, and specific antibody levels at various time points in infected piglets. Our results revealed synergistic effects in HP-PRRSV–Gps coinfection, which increase the severity of clinical signs compared with single infections. Therefore, in the unavoidable situation of Gps infection in piglets, necessary measures must be made to prevent and control secondary infection of HP-PRRSV, which can save huge economic losses to the pork industry. ABSTRACT: Glaesserella parasuis (Gps), Gram-negative bacteria, are a universal respiratory-disease-causing pathogen in swine that colonize the upper respiratory tract. Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2HP-PRRSV2) and Gps coinfections are epidemics in China, but little is known about the influence of concurrent coinfection on disease severity and inflammatory responses. Herein, we studied the effects of secondary HP-PRRS infection on clinical symptoms, pathological changes, pathogen load, and inflammatory response of Gps coinfection in the upper respiratory tract of piglets. All coinfected piglets (HP-PRRSV2 + Gps) displayed fever and severe lesions in the lungs, while fever was present in only a few animals with a single infection (HP-PRRSV2 or Gps). Additionally, HP-PRRSV2 and Gps loading in nasal swabs and blood and lung tissue samples was significantly increased in the coinfected group. Necropsy data showed that coinfected piglets suffered from severe lung damage and had significantly higher antibody titers of HP-PRRSV2 or Gps than single-infected piglets. Moreover, the serum and lung concentrations of inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8) were also significantly higher in coinfected piglets than in those infected with HP-PRRSV2 or Gps alone. In conclusion, our results show that HP-PRRSV2 promotes the shedding and replication of Gps, and their coinfection in the upper respiratory tract aggravates the clinical symptoms and inflammatory responses, causing lung damage. Therefore, in the unavoidable situation of Gps infection in piglets, necessary measures must be made to prevent and control secondary infection with HP-PRRSV2, which can save huge economic losses to the pork industry.
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spelling pubmed-102229572023-05-28 Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets Guan, Zhixin Pang, Linlin Ouyang, Yan Jiang, Yifeng Zhang, Junjie Qiu, Yafeng Li, Zongjie Li, Beibei Liu, Ke Shao, Donghua Ma, Zhiyong Wei, Jianchao Vet Sci Article SIMPLE SUMMARY: Based on the fact that Gps are rooted in the upper respiratory tract of pigs, in order to investigate whether secondary infection with HP-PRRSV can exacerbate lung disease and chronic inflammation, our study was designed as follows. Our study randomly divided piglets into four groups: Gps + HP-PRRSV, Gps, HP-PRRSV, and controls. Piglets in the Gps + HP-PRRSV and Gps groups were infected through the intranasal route with the Gps W2 strain. The Gps + HP-PRRSV and HP-PRRSV groups were challenged with the HP-PRRSV HuN4 strain by intramuscular injection and intranasally at 5 days after the initial exposure to Gps. Alternatively, the control group animals received phosphate-buffered saline (PBS). Then, we observed the influence of HP-PRRSV–Gps coinfection on clinical outcomes, pathogen shedding and loading, cytokine production, and specific antibody levels at various time points in infected piglets. Our results revealed synergistic effects in HP-PRRSV–Gps coinfection, which increase the severity of clinical signs compared with single infections. Therefore, in the unavoidable situation of Gps infection in piglets, necessary measures must be made to prevent and control secondary infection of HP-PRRSV, which can save huge economic losses to the pork industry. ABSTRACT: Glaesserella parasuis (Gps), Gram-negative bacteria, are a universal respiratory-disease-causing pathogen in swine that colonize the upper respiratory tract. Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2HP-PRRSV2) and Gps coinfections are epidemics in China, but little is known about the influence of concurrent coinfection on disease severity and inflammatory responses. Herein, we studied the effects of secondary HP-PRRS infection on clinical symptoms, pathological changes, pathogen load, and inflammatory response of Gps coinfection in the upper respiratory tract of piglets. All coinfected piglets (HP-PRRSV2 + Gps) displayed fever and severe lesions in the lungs, while fever was present in only a few animals with a single infection (HP-PRRSV2 or Gps). Additionally, HP-PRRSV2 and Gps loading in nasal swabs and blood and lung tissue samples was significantly increased in the coinfected group. Necropsy data showed that coinfected piglets suffered from severe lung damage and had significantly higher antibody titers of HP-PRRSV2 or Gps than single-infected piglets. Moreover, the serum and lung concentrations of inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8) were also significantly higher in coinfected piglets than in those infected with HP-PRRSV2 or Gps alone. In conclusion, our results show that HP-PRRSV2 promotes the shedding and replication of Gps, and their coinfection in the upper respiratory tract aggravates the clinical symptoms and inflammatory responses, causing lung damage. Therefore, in the unavoidable situation of Gps infection in piglets, necessary measures must be made to prevent and control secondary infection with HP-PRRSV2, which can save huge economic losses to the pork industry. MDPI 2023-05-20 /pmc/articles/PMC10222957/ /pubmed/37235448 http://dx.doi.org/10.3390/vetsci10050365 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guan, Zhixin
Pang, Linlin
Ouyang, Yan
Jiang, Yifeng
Zhang, Junjie
Qiu, Yafeng
Li, Zongjie
Li, Beibei
Liu, Ke
Shao, Donghua
Ma, Zhiyong
Wei, Jianchao
Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets
title Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets
title_full Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets
title_fullStr Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets
title_full_unstemmed Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets
title_short Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets
title_sort secondary highly pathogenic porcine reproductive and respiratory syndrome virus (hp-prrsv2) infection augments inflammatory responses, clinical outcomes, and pathogen load in glaesserella-parasuis-infected piglets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222957/
https://www.ncbi.nlm.nih.gov/pubmed/37235448
http://dx.doi.org/10.3390/vetsci10050365
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