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Phylogeny-Related Variations in Venomics: A Test in a Subset of Habu Snakes (Protobothrops)
We conducted a comparative analysis to unveil the divergence among venoms from a subset of Old World habu snakes (Protobothrops) in terms of venomic profiles and toxicological and enzymatic activities. A total of 14 protein families were identified in the venoms from these habu snakes, and 11 of the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223207/ https://www.ncbi.nlm.nih.gov/pubmed/37235384 http://dx.doi.org/10.3390/toxins15050350 |
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author | Zhao, Hong-Yan He, Na Sun, Yan Wang, Yong-Chen Zhang, Hao-Bing Chen, Hui-Hui Zhang, Ya-Qi Gao, Jian-Fang |
author_facet | Zhao, Hong-Yan He, Na Sun, Yan Wang, Yong-Chen Zhang, Hao-Bing Chen, Hui-Hui Zhang, Ya-Qi Gao, Jian-Fang |
author_sort | Zhao, Hong-Yan |
collection | PubMed |
description | We conducted a comparative analysis to unveil the divergence among venoms from a subset of Old World habu snakes (Protobothrops) in terms of venomic profiles and toxicological and enzymatic activities. A total of 14 protein families were identified in the venoms from these habu snakes, and 11 of them were shared among these venoms. The venoms of five adult habu snakes were overwhelmingly dominated by SVMP (32.56 ± 13.94%), PLA(2) (22.93 ± 9.26%), and SVSP (16.27 ± 4.79%), with a total abundance of over 65%, while the subadult P. mangshanensis had an extremely low abundance of PLA(2) (1.23%) but a high abundance of CTL (51.47%), followed by SVMP (22.06%) and SVSP (10.90%). Apparent interspecific variations in lethality and enzymatic activities were also explored in habu snake venoms, but no variations in myotoxicity were found. Except for SVSP, the resemblance of the relatives within Protobothrops in other venom traits was estimated to deviate from Brownian motion evolution based on phylogenetic signals. A comparative analysis further validated that the degree of covariation between phylogeny and venom variation is evolutionarily labile and varies among clades of closely related snakes. Our findings indicate a high level of interspecific variation in the venom proteomes of habu snakes, both in the presence or absence and the relative abundance of venom protein families, and that these venoms might have evolved under a combination of adaptive and neutral mechanisms. |
format | Online Article Text |
id | pubmed-10223207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102232072023-05-28 Phylogeny-Related Variations in Venomics: A Test in a Subset of Habu Snakes (Protobothrops) Zhao, Hong-Yan He, Na Sun, Yan Wang, Yong-Chen Zhang, Hao-Bing Chen, Hui-Hui Zhang, Ya-Qi Gao, Jian-Fang Toxins (Basel) Article We conducted a comparative analysis to unveil the divergence among venoms from a subset of Old World habu snakes (Protobothrops) in terms of venomic profiles and toxicological and enzymatic activities. A total of 14 protein families were identified in the venoms from these habu snakes, and 11 of them were shared among these venoms. The venoms of five adult habu snakes were overwhelmingly dominated by SVMP (32.56 ± 13.94%), PLA(2) (22.93 ± 9.26%), and SVSP (16.27 ± 4.79%), with a total abundance of over 65%, while the subadult P. mangshanensis had an extremely low abundance of PLA(2) (1.23%) but a high abundance of CTL (51.47%), followed by SVMP (22.06%) and SVSP (10.90%). Apparent interspecific variations in lethality and enzymatic activities were also explored in habu snake venoms, but no variations in myotoxicity were found. Except for SVSP, the resemblance of the relatives within Protobothrops in other venom traits was estimated to deviate from Brownian motion evolution based on phylogenetic signals. A comparative analysis further validated that the degree of covariation between phylogeny and venom variation is evolutionarily labile and varies among clades of closely related snakes. Our findings indicate a high level of interspecific variation in the venom proteomes of habu snakes, both in the presence or absence and the relative abundance of venom protein families, and that these venoms might have evolved under a combination of adaptive and neutral mechanisms. MDPI 2023-05-21 /pmc/articles/PMC10223207/ /pubmed/37235384 http://dx.doi.org/10.3390/toxins15050350 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Hong-Yan He, Na Sun, Yan Wang, Yong-Chen Zhang, Hao-Bing Chen, Hui-Hui Zhang, Ya-Qi Gao, Jian-Fang Phylogeny-Related Variations in Venomics: A Test in a Subset of Habu Snakes (Protobothrops) |
title | Phylogeny-Related Variations in Venomics: A Test in a Subset of Habu Snakes (Protobothrops) |
title_full | Phylogeny-Related Variations in Venomics: A Test in a Subset of Habu Snakes (Protobothrops) |
title_fullStr | Phylogeny-Related Variations in Venomics: A Test in a Subset of Habu Snakes (Protobothrops) |
title_full_unstemmed | Phylogeny-Related Variations in Venomics: A Test in a Subset of Habu Snakes (Protobothrops) |
title_short | Phylogeny-Related Variations in Venomics: A Test in a Subset of Habu Snakes (Protobothrops) |
title_sort | phylogeny-related variations in venomics: a test in a subset of habu snakes (protobothrops) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223207/ https://www.ncbi.nlm.nih.gov/pubmed/37235384 http://dx.doi.org/10.3390/toxins15050350 |
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