Cargando…

A Metabolome Analysis and the Immunity of Phlomis purpurea against Phytophthora cinnamomi

Phlomis purpurea grows spontaneously in the southern Iberian Peninsula, namely in cork oak (Quercus suber) forests. In a previous transcriptome analysis, we reported on its immunity against Phytophthora cinnamomi. However, little is known about the involvement of secondary metabolites in the P. purp...

Descripción completa

Detalles Bibliográficos
Autores principales: Neves, Dina, Figueiredo, Andreia, Maia, Marisa, Laczko, Endre, Pais, Maria Salomé, Cravador, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223286/
https://www.ncbi.nlm.nih.gov/pubmed/37653845
http://dx.doi.org/10.3390/plants12101929
_version_ 1785049905220288512
author Neves, Dina
Figueiredo, Andreia
Maia, Marisa
Laczko, Endre
Pais, Maria Salomé
Cravador, Alfredo
author_facet Neves, Dina
Figueiredo, Andreia
Maia, Marisa
Laczko, Endre
Pais, Maria Salomé
Cravador, Alfredo
author_sort Neves, Dina
collection PubMed
description Phlomis purpurea grows spontaneously in the southern Iberian Peninsula, namely in cork oak (Quercus suber) forests. In a previous transcriptome analysis, we reported on its immunity against Phytophthora cinnamomi. However, little is known about the involvement of secondary metabolites in the P. purpurea defense response. It is known, though, that root exudates are toxic to this pathogen. To understand the involvement of secondary metabolites in the defense of P. purpurea, a metabolome analysis was performed using the leaves and roots of plants challenged with the pathogen for over 72 h. The putatively identified compounds were constitutively produced. Alkaloids, fatty acids, flavonoids, glucosinolates, polyketides, prenol lipids, phenylpropanoids, sterols, and terpenoids were differentially produced in these leaves and roots along the experiment timescale. It must be emphasized that the constitutive production of taurine in leaves and its increase soon after challenging suggests its role in P. purpurea immunity against the stress imposed by the oomycete. The rapid increase in secondary metabolite production by this plant species accounts for a concerted action of multiple compounds and genes on the innate protection of Phlomis purpurea against Phytophthora cinnamomi. The combination of the metabolome with the transcriptome data previously disclosed confirms the mentioned innate immunity of this plant against a devastating pathogen. It suggests its potential as an antagonist in phytopathogens’ biological control. Its application in green forestry/agriculture is therefore possible.
format Online
Article
Text
id pubmed-10223286
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102232862023-05-28 A Metabolome Analysis and the Immunity of Phlomis purpurea against Phytophthora cinnamomi Neves, Dina Figueiredo, Andreia Maia, Marisa Laczko, Endre Pais, Maria Salomé Cravador, Alfredo Plants (Basel) Article Phlomis purpurea grows spontaneously in the southern Iberian Peninsula, namely in cork oak (Quercus suber) forests. In a previous transcriptome analysis, we reported on its immunity against Phytophthora cinnamomi. However, little is known about the involvement of secondary metabolites in the P. purpurea defense response. It is known, though, that root exudates are toxic to this pathogen. To understand the involvement of secondary metabolites in the defense of P. purpurea, a metabolome analysis was performed using the leaves and roots of plants challenged with the pathogen for over 72 h. The putatively identified compounds were constitutively produced. Alkaloids, fatty acids, flavonoids, glucosinolates, polyketides, prenol lipids, phenylpropanoids, sterols, and terpenoids were differentially produced in these leaves and roots along the experiment timescale. It must be emphasized that the constitutive production of taurine in leaves and its increase soon after challenging suggests its role in P. purpurea immunity against the stress imposed by the oomycete. The rapid increase in secondary metabolite production by this plant species accounts for a concerted action of multiple compounds and genes on the innate protection of Phlomis purpurea against Phytophthora cinnamomi. The combination of the metabolome with the transcriptome data previously disclosed confirms the mentioned innate immunity of this plant against a devastating pathogen. It suggests its potential as an antagonist in phytopathogens’ biological control. Its application in green forestry/agriculture is therefore possible. MDPI 2023-05-09 /pmc/articles/PMC10223286/ /pubmed/37653845 http://dx.doi.org/10.3390/plants12101929 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Neves, Dina
Figueiredo, Andreia
Maia, Marisa
Laczko, Endre
Pais, Maria Salomé
Cravador, Alfredo
A Metabolome Analysis and the Immunity of Phlomis purpurea against Phytophthora cinnamomi
title A Metabolome Analysis and the Immunity of Phlomis purpurea against Phytophthora cinnamomi
title_full A Metabolome Analysis and the Immunity of Phlomis purpurea against Phytophthora cinnamomi
title_fullStr A Metabolome Analysis and the Immunity of Phlomis purpurea against Phytophthora cinnamomi
title_full_unstemmed A Metabolome Analysis and the Immunity of Phlomis purpurea against Phytophthora cinnamomi
title_short A Metabolome Analysis and the Immunity of Phlomis purpurea against Phytophthora cinnamomi
title_sort metabolome analysis and the immunity of phlomis purpurea against phytophthora cinnamomi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223286/
https://www.ncbi.nlm.nih.gov/pubmed/37653845
http://dx.doi.org/10.3390/plants12101929
work_keys_str_mv AT nevesdina ametabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT figueiredoandreia ametabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT maiamarisa ametabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT laczkoendre ametabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT paismariasalome ametabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT cravadoralfredo ametabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT nevesdina metabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT figueiredoandreia metabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT maiamarisa metabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT laczkoendre metabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT paismariasalome metabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi
AT cravadoralfredo metabolomeanalysisandtheimmunityofphlomispurpureaagainstphytophthoracinnamomi