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Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment

Bacterial vaginosis is an infectious disease that has significantly affected women’s health. Metronidazole has been widely used as a drug for treating bacterial vaginosis. Nevertheless, the currently available therapies have been found to be inefficient and inconvenient. Here, we developed the combi...

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Autores principales: Permana, Andi Dian, Asri, Rangga Meidianto, Amir, Muhammad Nur, Himawan, Achmad, Arjuna, Andi, Juniarti, Nana, Utami, Rifka Nurul, Mardikasari, Sandra Aulia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223408/
https://www.ncbi.nlm.nih.gov/pubmed/37242771
http://dx.doi.org/10.3390/pharmaceutics15051529
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author Permana, Andi Dian
Asri, Rangga Meidianto
Amir, Muhammad Nur
Himawan, Achmad
Arjuna, Andi
Juniarti, Nana
Utami, Rifka Nurul
Mardikasari, Sandra Aulia
author_facet Permana, Andi Dian
Asri, Rangga Meidianto
Amir, Muhammad Nur
Himawan, Achmad
Arjuna, Andi
Juniarti, Nana
Utami, Rifka Nurul
Mardikasari, Sandra Aulia
author_sort Permana, Andi Dian
collection PubMed
description Bacterial vaginosis is an infectious disease that has significantly affected women’s health. Metronidazole has been widely used as a drug for treating bacterial vaginosis. Nevertheless, the currently available therapies have been found to be inefficient and inconvenient. Here, we developed the combination approach of gel flake and thermoresponsive hydrogel systems. The gel flakes were prepared using gellan gum and chitosan, showing that the incorporation of metronidazole was able to provide a sustained release pattern for 24 h with an entrapment efficiency of >90%. Moreover, the gel flakes were incorporated into Pluronics-based thermoresponsive hydrogel using the combination of Pluronic F127 and F68. The hydrogels were found to exhibit the desired thermoresponsive properties, showing sol-gel transition at vaginal temperature. Following the addition of sodium alginate as a mucoadhesive agent, the hydrogel was retained in the vaginal tissue for more than 8 h, with more than 5 mg of metronidazole retained in the ex vivo evaluation. Finally, using the bacterial vaginosis infection model in rats, this approach could decrease the viability of Escherichia coli and Staphylococcus aureus with reduction percentages of more than 95% after 3 days of treatment, with the healing ability similar to normal vaginal tissue. In conclusion, this study offers an effective approach for the treatment of bacterial vaginosis.
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spelling pubmed-102234082023-05-28 Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment Permana, Andi Dian Asri, Rangga Meidianto Amir, Muhammad Nur Himawan, Achmad Arjuna, Andi Juniarti, Nana Utami, Rifka Nurul Mardikasari, Sandra Aulia Pharmaceutics Article Bacterial vaginosis is an infectious disease that has significantly affected women’s health. Metronidazole has been widely used as a drug for treating bacterial vaginosis. Nevertheless, the currently available therapies have been found to be inefficient and inconvenient. Here, we developed the combination approach of gel flake and thermoresponsive hydrogel systems. The gel flakes were prepared using gellan gum and chitosan, showing that the incorporation of metronidazole was able to provide a sustained release pattern for 24 h with an entrapment efficiency of >90%. Moreover, the gel flakes were incorporated into Pluronics-based thermoresponsive hydrogel using the combination of Pluronic F127 and F68. The hydrogels were found to exhibit the desired thermoresponsive properties, showing sol-gel transition at vaginal temperature. Following the addition of sodium alginate as a mucoadhesive agent, the hydrogel was retained in the vaginal tissue for more than 8 h, with more than 5 mg of metronidazole retained in the ex vivo evaluation. Finally, using the bacterial vaginosis infection model in rats, this approach could decrease the viability of Escherichia coli and Staphylococcus aureus with reduction percentages of more than 95% after 3 days of treatment, with the healing ability similar to normal vaginal tissue. In conclusion, this study offers an effective approach for the treatment of bacterial vaginosis. MDPI 2023-05-18 /pmc/articles/PMC10223408/ /pubmed/37242771 http://dx.doi.org/10.3390/pharmaceutics15051529 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Permana, Andi Dian
Asri, Rangga Meidianto
Amir, Muhammad Nur
Himawan, Achmad
Arjuna, Andi
Juniarti, Nana
Utami, Rifka Nurul
Mardikasari, Sandra Aulia
Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment
title Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment
title_full Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment
title_fullStr Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment
title_full_unstemmed Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment
title_short Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment
title_sort development of thermoresponsive hydrogels with mucoadhesion properties loaded with metronidazole gel-flakes for improved bacterial vaginosis treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223408/
https://www.ncbi.nlm.nih.gov/pubmed/37242771
http://dx.doi.org/10.3390/pharmaceutics15051529
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