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Chemical Composition and Antimicrobial Properties of Honey Bee Venom

Due to its great medical and pharmaceutical importance, honey bee venom is considered to be well characterized both chemically and in terms of biomedical activity. However, this study shows that our knowledge of the composition and antimicrobial properties of Apis mellifera venom is incomplete. In t...

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Autores principales: Isidorov, Valery, Zalewski, Adam, Zambrowski, Grzegorz, Swiecicka, Izabela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223701/
https://www.ncbi.nlm.nih.gov/pubmed/37241876
http://dx.doi.org/10.3390/molecules28104135
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author Isidorov, Valery
Zalewski, Adam
Zambrowski, Grzegorz
Swiecicka, Izabela
author_facet Isidorov, Valery
Zalewski, Adam
Zambrowski, Grzegorz
Swiecicka, Izabela
author_sort Isidorov, Valery
collection PubMed
description Due to its great medical and pharmaceutical importance, honey bee venom is considered to be well characterized both chemically and in terms of biomedical activity. However, this study shows that our knowledge of the composition and antimicrobial properties of Apis mellifera venom is incomplete. In this work, the composition of volatile and extractive components of dry and fresh bee venom (BV) was determined by GC-MS, as well as antimicrobial activity against seven types of pathogenic microorganisms. One-hundred and forty-nine organic C(1)–C(19) compounds of different classes were found in the volatile secretions of the studied BV samples. One-hundred and fifty-two organic C(2)–C(36) compounds were registered in ether extracts, and 201 compounds were identified in methanol extracts. More than half of these compounds are new to BV. In microbiological tests involving four species of pathogenic Gram-positive and two species of Gram-negative bacteria, as well as one species of pathogenic fungi, the values of the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) were determined for samples of dry BV, as well as ether and methanol extracts from it. Gram-positive bacteria show the greatest sensitivity to the action of all tested drugs. The minimum MIC values for Gram-positive bacteria in the range of 0.12–7.63 ng mL(−1) were recorded for whole BV, while for the methanol extract they were 0.49–125 ng mL(−1). The ether extracts had a weaker effect on the tested bacteria (MIC values 31.25–500 ng mL(−1)). Interestingly, Escherichia coli was more sensitive (MIC 7.63–500 ng mL(−1)) to the action of bee venom compared to Pseudomonas aeruginosa (MIC ≥ 500 ng mL(−1)). The results of the tests carried out indicate that the antimicrobial effect of BV is associated with the presence of not only peptides, such as melittin, but also low molecular weight metabolites.
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spelling pubmed-102237012023-05-28 Chemical Composition and Antimicrobial Properties of Honey Bee Venom Isidorov, Valery Zalewski, Adam Zambrowski, Grzegorz Swiecicka, Izabela Molecules Article Due to its great medical and pharmaceutical importance, honey bee venom is considered to be well characterized both chemically and in terms of biomedical activity. However, this study shows that our knowledge of the composition and antimicrobial properties of Apis mellifera venom is incomplete. In this work, the composition of volatile and extractive components of dry and fresh bee venom (BV) was determined by GC-MS, as well as antimicrobial activity against seven types of pathogenic microorganisms. One-hundred and forty-nine organic C(1)–C(19) compounds of different classes were found in the volatile secretions of the studied BV samples. One-hundred and fifty-two organic C(2)–C(36) compounds were registered in ether extracts, and 201 compounds were identified in methanol extracts. More than half of these compounds are new to BV. In microbiological tests involving four species of pathogenic Gram-positive and two species of Gram-negative bacteria, as well as one species of pathogenic fungi, the values of the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) were determined for samples of dry BV, as well as ether and methanol extracts from it. Gram-positive bacteria show the greatest sensitivity to the action of all tested drugs. The minimum MIC values for Gram-positive bacteria in the range of 0.12–7.63 ng mL(−1) were recorded for whole BV, while for the methanol extract they were 0.49–125 ng mL(−1). The ether extracts had a weaker effect on the tested bacteria (MIC values 31.25–500 ng mL(−1)). Interestingly, Escherichia coli was more sensitive (MIC 7.63–500 ng mL(−1)) to the action of bee venom compared to Pseudomonas aeruginosa (MIC ≥ 500 ng mL(−1)). The results of the tests carried out indicate that the antimicrobial effect of BV is associated with the presence of not only peptides, such as melittin, but also low molecular weight metabolites. MDPI 2023-05-17 /pmc/articles/PMC10223701/ /pubmed/37241876 http://dx.doi.org/10.3390/molecules28104135 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Isidorov, Valery
Zalewski, Adam
Zambrowski, Grzegorz
Swiecicka, Izabela
Chemical Composition and Antimicrobial Properties of Honey Bee Venom
title Chemical Composition and Antimicrobial Properties of Honey Bee Venom
title_full Chemical Composition and Antimicrobial Properties of Honey Bee Venom
title_fullStr Chemical Composition and Antimicrobial Properties of Honey Bee Venom
title_full_unstemmed Chemical Composition and Antimicrobial Properties of Honey Bee Venom
title_short Chemical Composition and Antimicrobial Properties of Honey Bee Venom
title_sort chemical composition and antimicrobial properties of honey bee venom
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223701/
https://www.ncbi.nlm.nih.gov/pubmed/37241876
http://dx.doi.org/10.3390/molecules28104135
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