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Association of a high-fat diet with I-FABP as a biomarker of intestinal barrier dysfunction driven by metabolic changes in Wistar rats

BACKGROUND: The epithelial lining of the gut expresses intestinal fatty-acid binding proteins (I-FABPs), which increase in circulation and in plasma concentration during intestinal damage. From the perspective of obesity, the consumption of a diet rich in fat causes a disruption in the integrity of...

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Autores principales: Mahmood, Aisha, Faisal, Muhammad Naeem, Khan, Junaid Ali, Muzaffar, Humaira, Muhammad, Faqir, Hussain, Jazib, Aslam, Jawad, Anwar, Haseeb
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223920/
https://www.ncbi.nlm.nih.gov/pubmed/37237272
http://dx.doi.org/10.1186/s12944-023-01837-9
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author Mahmood, Aisha
Faisal, Muhammad Naeem
Khan, Junaid Ali
Muzaffar, Humaira
Muhammad, Faqir
Hussain, Jazib
Aslam, Jawad
Anwar, Haseeb
author_facet Mahmood, Aisha
Faisal, Muhammad Naeem
Khan, Junaid Ali
Muzaffar, Humaira
Muhammad, Faqir
Hussain, Jazib
Aslam, Jawad
Anwar, Haseeb
author_sort Mahmood, Aisha
collection PubMed
description BACKGROUND: The epithelial lining of the gut expresses intestinal fatty-acid binding proteins (I-FABPs), which increase in circulation and in plasma concentration during intestinal damage. From the perspective of obesity, the consumption of a diet rich in fat causes a disruption in the integrity of the gut barrier and an increase in its permeability. HYPOTHESIS: There is an association between the expression of I-FABP in the gut and various metabolic changes induced by a high-fat (HF) diet. METHODS: Wistar albino rats (n = 90) were divided into three groups (n = 30 per group), viz. One control and two HF diet groups (15 and 30%, respectively) were maintained for 6 weeks. Blood samples were thus collected to evaluate the lipid profile, blood glucose level and other biochemical tests. Tissue sampling was conducted to perform fat staining and immunohistochemistry. RESULTS: HF diet-fed rats developed adiposity, insulin resistance, leptin resistance, dyslipidemia, and increased expression of I-FABP in the small intestine compared to the control group. Increased I-FABP expression in the ileal region of the intestine is correlated significantly with higher fat contents in the diet, indicating that higher I-FABP expression occurs due to increased demand of enterocytes to transport lipids, leading to metabolic alterations. CONCLUSION: In summary, there is an association between the expression of I-FABP and HF diet-induced metabolic alterations, indicating that I-FABP can be used as a biomarker for intestinal barrier dysfunction. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12944-023-01837-9.
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spelling pubmed-102239202023-05-28 Association of a high-fat diet with I-FABP as a biomarker of intestinal barrier dysfunction driven by metabolic changes in Wistar rats Mahmood, Aisha Faisal, Muhammad Naeem Khan, Junaid Ali Muzaffar, Humaira Muhammad, Faqir Hussain, Jazib Aslam, Jawad Anwar, Haseeb Lipids Health Dis Research BACKGROUND: The epithelial lining of the gut expresses intestinal fatty-acid binding proteins (I-FABPs), which increase in circulation and in plasma concentration during intestinal damage. From the perspective of obesity, the consumption of a diet rich in fat causes a disruption in the integrity of the gut barrier and an increase in its permeability. HYPOTHESIS: There is an association between the expression of I-FABP in the gut and various metabolic changes induced by a high-fat (HF) diet. METHODS: Wistar albino rats (n = 90) were divided into three groups (n = 30 per group), viz. One control and two HF diet groups (15 and 30%, respectively) were maintained for 6 weeks. Blood samples were thus collected to evaluate the lipid profile, blood glucose level and other biochemical tests. Tissue sampling was conducted to perform fat staining and immunohistochemistry. RESULTS: HF diet-fed rats developed adiposity, insulin resistance, leptin resistance, dyslipidemia, and increased expression of I-FABP in the small intestine compared to the control group. Increased I-FABP expression in the ileal region of the intestine is correlated significantly with higher fat contents in the diet, indicating that higher I-FABP expression occurs due to increased demand of enterocytes to transport lipids, leading to metabolic alterations. CONCLUSION: In summary, there is an association between the expression of I-FABP and HF diet-induced metabolic alterations, indicating that I-FABP can be used as a biomarker for intestinal barrier dysfunction. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12944-023-01837-9. BioMed Central 2023-05-27 /pmc/articles/PMC10223920/ /pubmed/37237272 http://dx.doi.org/10.1186/s12944-023-01837-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mahmood, Aisha
Faisal, Muhammad Naeem
Khan, Junaid Ali
Muzaffar, Humaira
Muhammad, Faqir
Hussain, Jazib
Aslam, Jawad
Anwar, Haseeb
Association of a high-fat diet with I-FABP as a biomarker of intestinal barrier dysfunction driven by metabolic changes in Wistar rats
title Association of a high-fat diet with I-FABP as a biomarker of intestinal barrier dysfunction driven by metabolic changes in Wistar rats
title_full Association of a high-fat diet with I-FABP as a biomarker of intestinal barrier dysfunction driven by metabolic changes in Wistar rats
title_fullStr Association of a high-fat diet with I-FABP as a biomarker of intestinal barrier dysfunction driven by metabolic changes in Wistar rats
title_full_unstemmed Association of a high-fat diet with I-FABP as a biomarker of intestinal barrier dysfunction driven by metabolic changes in Wistar rats
title_short Association of a high-fat diet with I-FABP as a biomarker of intestinal barrier dysfunction driven by metabolic changes in Wistar rats
title_sort association of a high-fat diet with i-fabp as a biomarker of intestinal barrier dysfunction driven by metabolic changes in wistar rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223920/
https://www.ncbi.nlm.nih.gov/pubmed/37237272
http://dx.doi.org/10.1186/s12944-023-01837-9
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