Cargando…
Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model
Beta-caryophyllene has demonstrated anti-inflammatory effects in a variety of conditions, including interstitial cystitis. These effects are mediated primarily via the activation of the cannabinoid type 2 receptor. Additional antibacterial properties have recently been suggested, leading to our inve...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223983/ https://www.ncbi.nlm.nih.gov/pubmed/37241885 http://dx.doi.org/10.3390/molecules28104144 |
_version_ | 1785050069369618432 |
---|---|
author | Dickson, Kayle Scott, Cassidy White, Hannah Zhou, Juan Kelly, Melanie Lehmann, Christian |
author_facet | Dickson, Kayle Scott, Cassidy White, Hannah Zhou, Juan Kelly, Melanie Lehmann, Christian |
author_sort | Dickson, Kayle |
collection | PubMed |
description | Beta-caryophyllene has demonstrated anti-inflammatory effects in a variety of conditions, including interstitial cystitis. These effects are mediated primarily via the activation of the cannabinoid type 2 receptor. Additional antibacterial properties have recently been suggested, leading to our investigation of the effects of beta-caryophyllene in a murine model of urinary tract infection (UTI). Female BALB/c mice were intravesically inoculated with uropathogenic Escherichia coli CFT073. The mice received either beta-caryophyllene, antibiotic treatment using fosfomycin, or combination therapy. After 6, 24, or 72 h, the mice were evaluated for bacterial burden in the bladder and changes in pain and behavioral responses using von Frey esthesiometry. In the 24 h model, the anti-inflammatory effects of beta-caryophyllene were also assessed using intravital microscopy. The mice established a robust UTI by 24 h. Altered behavioral responses persisted 72 h post infection. Treatment with beta-caryophyllene resulted in a significant reduction in the bacterial burden in urine and bladder tissues 24 h post UTI induction and significant improvements in behavioral responses and intravital microscopy parameters, representing reduced inflammation in the bladder. This study demonstrates the utility of beta-caryophyllene as a new adjunct therapy for the management of UTI. |
format | Online Article Text |
id | pubmed-10223983 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102239832023-05-28 Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model Dickson, Kayle Scott, Cassidy White, Hannah Zhou, Juan Kelly, Melanie Lehmann, Christian Molecules Article Beta-caryophyllene has demonstrated anti-inflammatory effects in a variety of conditions, including interstitial cystitis. These effects are mediated primarily via the activation of the cannabinoid type 2 receptor. Additional antibacterial properties have recently been suggested, leading to our investigation of the effects of beta-caryophyllene in a murine model of urinary tract infection (UTI). Female BALB/c mice were intravesically inoculated with uropathogenic Escherichia coli CFT073. The mice received either beta-caryophyllene, antibiotic treatment using fosfomycin, or combination therapy. After 6, 24, or 72 h, the mice were evaluated for bacterial burden in the bladder and changes in pain and behavioral responses using von Frey esthesiometry. In the 24 h model, the anti-inflammatory effects of beta-caryophyllene were also assessed using intravital microscopy. The mice established a robust UTI by 24 h. Altered behavioral responses persisted 72 h post infection. Treatment with beta-caryophyllene resulted in a significant reduction in the bacterial burden in urine and bladder tissues 24 h post UTI induction and significant improvements in behavioral responses and intravital microscopy parameters, representing reduced inflammation in the bladder. This study demonstrates the utility of beta-caryophyllene as a new adjunct therapy for the management of UTI. MDPI 2023-05-17 /pmc/articles/PMC10223983/ /pubmed/37241885 http://dx.doi.org/10.3390/molecules28104144 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dickson, Kayle Scott, Cassidy White, Hannah Zhou, Juan Kelly, Melanie Lehmann, Christian Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model |
title | Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model |
title_full | Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model |
title_fullStr | Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model |
title_full_unstemmed | Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model |
title_short | Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model |
title_sort | antibacterial and analgesic properties of beta-caryophyllene in a murine urinary tract infection model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223983/ https://www.ncbi.nlm.nih.gov/pubmed/37241885 http://dx.doi.org/10.3390/molecules28104144 |
work_keys_str_mv | AT dicksonkayle antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel AT scottcassidy antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel AT whitehannah antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel AT zhoujuan antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel AT kellymelanie antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel AT lehmannchristian antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel |