Cargando…

Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model

Beta-caryophyllene has demonstrated anti-inflammatory effects in a variety of conditions, including interstitial cystitis. These effects are mediated primarily via the activation of the cannabinoid type 2 receptor. Additional antibacterial properties have recently been suggested, leading to our inve...

Descripción completa

Detalles Bibliográficos
Autores principales: Dickson, Kayle, Scott, Cassidy, White, Hannah, Zhou, Juan, Kelly, Melanie, Lehmann, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223983/
https://www.ncbi.nlm.nih.gov/pubmed/37241885
http://dx.doi.org/10.3390/molecules28104144
_version_ 1785050069369618432
author Dickson, Kayle
Scott, Cassidy
White, Hannah
Zhou, Juan
Kelly, Melanie
Lehmann, Christian
author_facet Dickson, Kayle
Scott, Cassidy
White, Hannah
Zhou, Juan
Kelly, Melanie
Lehmann, Christian
author_sort Dickson, Kayle
collection PubMed
description Beta-caryophyllene has demonstrated anti-inflammatory effects in a variety of conditions, including interstitial cystitis. These effects are mediated primarily via the activation of the cannabinoid type 2 receptor. Additional antibacterial properties have recently been suggested, leading to our investigation of the effects of beta-caryophyllene in a murine model of urinary tract infection (UTI). Female BALB/c mice were intravesically inoculated with uropathogenic Escherichia coli CFT073. The mice received either beta-caryophyllene, antibiotic treatment using fosfomycin, or combination therapy. After 6, 24, or 72 h, the mice were evaluated for bacterial burden in the bladder and changes in pain and behavioral responses using von Frey esthesiometry. In the 24 h model, the anti-inflammatory effects of beta-caryophyllene were also assessed using intravital microscopy. The mice established a robust UTI by 24 h. Altered behavioral responses persisted 72 h post infection. Treatment with beta-caryophyllene resulted in a significant reduction in the bacterial burden in urine and bladder tissues 24 h post UTI induction and significant improvements in behavioral responses and intravital microscopy parameters, representing reduced inflammation in the bladder. This study demonstrates the utility of beta-caryophyllene as a new adjunct therapy for the management of UTI.
format Online
Article
Text
id pubmed-10223983
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102239832023-05-28 Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model Dickson, Kayle Scott, Cassidy White, Hannah Zhou, Juan Kelly, Melanie Lehmann, Christian Molecules Article Beta-caryophyllene has demonstrated anti-inflammatory effects in a variety of conditions, including interstitial cystitis. These effects are mediated primarily via the activation of the cannabinoid type 2 receptor. Additional antibacterial properties have recently been suggested, leading to our investigation of the effects of beta-caryophyllene in a murine model of urinary tract infection (UTI). Female BALB/c mice were intravesically inoculated with uropathogenic Escherichia coli CFT073. The mice received either beta-caryophyllene, antibiotic treatment using fosfomycin, or combination therapy. After 6, 24, or 72 h, the mice were evaluated for bacterial burden in the bladder and changes in pain and behavioral responses using von Frey esthesiometry. In the 24 h model, the anti-inflammatory effects of beta-caryophyllene were also assessed using intravital microscopy. The mice established a robust UTI by 24 h. Altered behavioral responses persisted 72 h post infection. Treatment with beta-caryophyllene resulted in a significant reduction in the bacterial burden in urine and bladder tissues 24 h post UTI induction and significant improvements in behavioral responses and intravital microscopy parameters, representing reduced inflammation in the bladder. This study demonstrates the utility of beta-caryophyllene as a new adjunct therapy for the management of UTI. MDPI 2023-05-17 /pmc/articles/PMC10223983/ /pubmed/37241885 http://dx.doi.org/10.3390/molecules28104144 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dickson, Kayle
Scott, Cassidy
White, Hannah
Zhou, Juan
Kelly, Melanie
Lehmann, Christian
Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model
title Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model
title_full Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model
title_fullStr Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model
title_full_unstemmed Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model
title_short Antibacterial and Analgesic Properties of Beta-Caryophyllene in a Murine Urinary Tract Infection Model
title_sort antibacterial and analgesic properties of beta-caryophyllene in a murine urinary tract infection model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223983/
https://www.ncbi.nlm.nih.gov/pubmed/37241885
http://dx.doi.org/10.3390/molecules28104144
work_keys_str_mv AT dicksonkayle antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel
AT scottcassidy antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel
AT whitehannah antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel
AT zhoujuan antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel
AT kellymelanie antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel
AT lehmannchristian antibacterialandanalgesicpropertiesofbetacaryophylleneinamurineurinarytractinfectionmodel