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Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil

Dengue fever is among the most significant public health concerns in Brazil. To date, the highest number of Dengue notifications in the Americas has been reported in Brazil, with cases accounting for a total number of 3,418,796 reported cases as of mid-December 2022. Furthermore, the northeastern re...

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Autores principales: Fritsch, Hegger, Moreno, Keldenn, Lima, Italo Andrade Barbosa, Santos, Cleiton Silva, Costa, Bernardo Gratival Gouvea, de Almeida, Breno Lima, dos Santos, Ronald Alves, Francisco, Marcos Vinicius Lima de Oliveira, Sampaio, Maria Paula Souza, de Lima, Maricelia Maia, Pereira, Felicidade Mota, Fonseca, Vagner, Tosta, Stephane, Xavier, Joilson, de Oliveira, Carla, Adelino, Talita, de Mello, Arabela Leal e Silva, Gräf, Tiago, Alcantara, Luiz Carlos Junior, Giovanetti, Marta, de Siqueira, Isadora Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224011/
https://www.ncbi.nlm.nih.gov/pubmed/37243159
http://dx.doi.org/10.3390/v15051073
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author Fritsch, Hegger
Moreno, Keldenn
Lima, Italo Andrade Barbosa
Santos, Cleiton Silva
Costa, Bernardo Gratival Gouvea
de Almeida, Breno Lima
dos Santos, Ronald Alves
Francisco, Marcos Vinicius Lima de Oliveira
Sampaio, Maria Paula Souza
de Lima, Maricelia Maia
Pereira, Felicidade Mota
Fonseca, Vagner
Tosta, Stephane
Xavier, Joilson
de Oliveira, Carla
Adelino, Talita
de Mello, Arabela Leal e Silva
Gräf, Tiago
Alcantara, Luiz Carlos Junior
Giovanetti, Marta
de Siqueira, Isadora Cristina
author_facet Fritsch, Hegger
Moreno, Keldenn
Lima, Italo Andrade Barbosa
Santos, Cleiton Silva
Costa, Bernardo Gratival Gouvea
de Almeida, Breno Lima
dos Santos, Ronald Alves
Francisco, Marcos Vinicius Lima de Oliveira
Sampaio, Maria Paula Souza
de Lima, Maricelia Maia
Pereira, Felicidade Mota
Fonseca, Vagner
Tosta, Stephane
Xavier, Joilson
de Oliveira, Carla
Adelino, Talita
de Mello, Arabela Leal e Silva
Gräf, Tiago
Alcantara, Luiz Carlos Junior
Giovanetti, Marta
de Siqueira, Isadora Cristina
author_sort Fritsch, Hegger
collection PubMed
description Dengue fever is among the most significant public health concerns in Brazil. To date, the highest number of Dengue notifications in the Americas has been reported in Brazil, with cases accounting for a total number of 3,418,796 reported cases as of mid-December 2022. Furthermore, the northeastern region of Brazil registered the second-highest incidence of Dengue fever in 2022. Due to the alarming epidemiological scenario, in this study, we used a combination of portable whole-genome sequencing, phylodynamic, and epidemiological analyses to reveal a novel DENV-1 genotype V clade and the persistence of DENV-2 genotype III in the region. We further report the presence of non-synonymous mutations associated with non-structural domains, especially the NS2A (non-structural protein 2A), as well as describe synonymous mutations in envelope and membrane proteins, distributed differently between clades. However, the absence of clinical data at the time of collection and notification, as well as the impossibility of monitoring patients in order to observe worsening or death, restricts our possibility of correlating mutational findings with possible clinical prognoses. Together, these results reinforce the crucial role of genomic surveillance to follow the evolution of circulating DENV strains and understand their spread across the region through inter-regional importation events, likely mediated by human mobility, and also the possible impacts on public health and outbreak management.
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spelling pubmed-102240112023-05-28 Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil Fritsch, Hegger Moreno, Keldenn Lima, Italo Andrade Barbosa Santos, Cleiton Silva Costa, Bernardo Gratival Gouvea de Almeida, Breno Lima dos Santos, Ronald Alves Francisco, Marcos Vinicius Lima de Oliveira Sampaio, Maria Paula Souza de Lima, Maricelia Maia Pereira, Felicidade Mota Fonseca, Vagner Tosta, Stephane Xavier, Joilson de Oliveira, Carla Adelino, Talita de Mello, Arabela Leal e Silva Gräf, Tiago Alcantara, Luiz Carlos Junior Giovanetti, Marta de Siqueira, Isadora Cristina Viruses Article Dengue fever is among the most significant public health concerns in Brazil. To date, the highest number of Dengue notifications in the Americas has been reported in Brazil, with cases accounting for a total number of 3,418,796 reported cases as of mid-December 2022. Furthermore, the northeastern region of Brazil registered the second-highest incidence of Dengue fever in 2022. Due to the alarming epidemiological scenario, in this study, we used a combination of portable whole-genome sequencing, phylodynamic, and epidemiological analyses to reveal a novel DENV-1 genotype V clade and the persistence of DENV-2 genotype III in the region. We further report the presence of non-synonymous mutations associated with non-structural domains, especially the NS2A (non-structural protein 2A), as well as describe synonymous mutations in envelope and membrane proteins, distributed differently between clades. However, the absence of clinical data at the time of collection and notification, as well as the impossibility of monitoring patients in order to observe worsening or death, restricts our possibility of correlating mutational findings with possible clinical prognoses. Together, these results reinforce the crucial role of genomic surveillance to follow the evolution of circulating DENV strains and understand their spread across the region through inter-regional importation events, likely mediated by human mobility, and also the possible impacts on public health and outbreak management. MDPI 2023-04-28 /pmc/articles/PMC10224011/ /pubmed/37243159 http://dx.doi.org/10.3390/v15051073 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fritsch, Hegger
Moreno, Keldenn
Lima, Italo Andrade Barbosa
Santos, Cleiton Silva
Costa, Bernardo Gratival Gouvea
de Almeida, Breno Lima
dos Santos, Ronald Alves
Francisco, Marcos Vinicius Lima de Oliveira
Sampaio, Maria Paula Souza
de Lima, Maricelia Maia
Pereira, Felicidade Mota
Fonseca, Vagner
Tosta, Stephane
Xavier, Joilson
de Oliveira, Carla
Adelino, Talita
de Mello, Arabela Leal e Silva
Gräf, Tiago
Alcantara, Luiz Carlos Junior
Giovanetti, Marta
de Siqueira, Isadora Cristina
Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil
title Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil
title_full Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil
title_fullStr Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil
title_full_unstemmed Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil
title_short Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil
title_sort phylogenetic reconstructions reveal the circulation of a novel dengue virus-1v clade and the persistence of a dengue virus-2 iii genotype in northeast brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224011/
https://www.ncbi.nlm.nih.gov/pubmed/37243159
http://dx.doi.org/10.3390/v15051073
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