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The Molecular Mechanism of GhbHLH121 in Response to Iron Deficiency in Cotton Seedlings
Iron deficiency caused by high pH of saline–alkali soil is a major source of abiotic stress affecting plant growth. However, the molecular mechanism underlying the iron deficiency response in cotton (Gossypium hirsutum) is poorly understood. In this study, we investigated the impacts of iron deficie...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224022/ https://www.ncbi.nlm.nih.gov/pubmed/37653872 http://dx.doi.org/10.3390/plants12101955 |
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author | Li, Jie Nie, Ke Wang, Luyao Zhao, Yongyan Qu, Mingnan Yang, Donglei Guan, Xueying |
author_facet | Li, Jie Nie, Ke Wang, Luyao Zhao, Yongyan Qu, Mingnan Yang, Donglei Guan, Xueying |
author_sort | Li, Jie |
collection | PubMed |
description | Iron deficiency caused by high pH of saline–alkali soil is a major source of abiotic stress affecting plant growth. However, the molecular mechanism underlying the iron deficiency response in cotton (Gossypium hirsutum) is poorly understood. In this study, we investigated the impacts of iron deficiency at the cotton seedling stage and elucidated the corresponding molecular regulation network, which centered on a hub gene GhbHLH121. Iron deficiency induced the expression of genes with roles in the response to iron deficiency, especially GhbHLH121. The suppression of GhbHLH121 with virus-induced gene silence technology reduced seedlings’ tolerance to iron deficiency, with low photosynthetic efficiency and severe damage to the structure of the chloroplast. Contrarily, ectopic expression of GhbHLH121 in Arabidopsis enhanced tolerance to iron deficiency. Further analysis of protein/protein interactions revealed that GhbHLH121 can interact with GhbHLH IVc and GhPYE. In addition, GhbHLH121 can directly activate the expression of GhbHLH38, GhFIT, and GhPYE independent of GhbHLH IVc. All told, GhbHLH121 is a positive regulator of the response to iron deficiency in cotton, directly regulating iron uptake as the upstream gene of GhFIT. Our results provide insight into the complex network of the iron deficiency response in cotton. |
format | Online Article Text |
id | pubmed-10224022 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102240222023-05-28 The Molecular Mechanism of GhbHLH121 in Response to Iron Deficiency in Cotton Seedlings Li, Jie Nie, Ke Wang, Luyao Zhao, Yongyan Qu, Mingnan Yang, Donglei Guan, Xueying Plants (Basel) Article Iron deficiency caused by high pH of saline–alkali soil is a major source of abiotic stress affecting plant growth. However, the molecular mechanism underlying the iron deficiency response in cotton (Gossypium hirsutum) is poorly understood. In this study, we investigated the impacts of iron deficiency at the cotton seedling stage and elucidated the corresponding molecular regulation network, which centered on a hub gene GhbHLH121. Iron deficiency induced the expression of genes with roles in the response to iron deficiency, especially GhbHLH121. The suppression of GhbHLH121 with virus-induced gene silence technology reduced seedlings’ tolerance to iron deficiency, with low photosynthetic efficiency and severe damage to the structure of the chloroplast. Contrarily, ectopic expression of GhbHLH121 in Arabidopsis enhanced tolerance to iron deficiency. Further analysis of protein/protein interactions revealed that GhbHLH121 can interact with GhbHLH IVc and GhPYE. In addition, GhbHLH121 can directly activate the expression of GhbHLH38, GhFIT, and GhPYE independent of GhbHLH IVc. All told, GhbHLH121 is a positive regulator of the response to iron deficiency in cotton, directly regulating iron uptake as the upstream gene of GhFIT. Our results provide insight into the complex network of the iron deficiency response in cotton. MDPI 2023-05-11 /pmc/articles/PMC10224022/ /pubmed/37653872 http://dx.doi.org/10.3390/plants12101955 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Jie Nie, Ke Wang, Luyao Zhao, Yongyan Qu, Mingnan Yang, Donglei Guan, Xueying The Molecular Mechanism of GhbHLH121 in Response to Iron Deficiency in Cotton Seedlings |
title | The Molecular Mechanism of GhbHLH121 in Response to Iron Deficiency in Cotton Seedlings |
title_full | The Molecular Mechanism of GhbHLH121 in Response to Iron Deficiency in Cotton Seedlings |
title_fullStr | The Molecular Mechanism of GhbHLH121 in Response to Iron Deficiency in Cotton Seedlings |
title_full_unstemmed | The Molecular Mechanism of GhbHLH121 in Response to Iron Deficiency in Cotton Seedlings |
title_short | The Molecular Mechanism of GhbHLH121 in Response to Iron Deficiency in Cotton Seedlings |
title_sort | molecular mechanism of ghbhlh121 in response to iron deficiency in cotton seedlings |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224022/ https://www.ncbi.nlm.nih.gov/pubmed/37653872 http://dx.doi.org/10.3390/plants12101955 |
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