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Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms

The main purpose of this study was to degrade total petroleum hydrocarbons (TPHs) from contaminated soil in batch microcosm reactors. Native soil fungi isolated from the same petroleum-polluted soil and ligninolytic fungal strains were screened and applied in the treatment of soil-contaminated micro...

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Autores principales: Daâssi, Dalel, Almaghrabi, Fatimah Qabil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224032/
https://www.ncbi.nlm.nih.gov/pubmed/37317325
http://dx.doi.org/10.3390/microorganisms11051351
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author Daâssi, Dalel
Almaghrabi, Fatimah Qabil
author_facet Daâssi, Dalel
Almaghrabi, Fatimah Qabil
author_sort Daâssi, Dalel
collection PubMed
description The main purpose of this study was to degrade total petroleum hydrocarbons (TPHs) from contaminated soil in batch microcosm reactors. Native soil fungi isolated from the same petroleum-polluted soil and ligninolytic fungal strains were screened and applied in the treatment of soil-contaminated microcosms in aerobic conditions. The bioaugmentation processes were carried out using selected hydrocarbonoclastic fungal strains in mono or co-cultures. Results demonstrated the petroleum-degrading potential of six fungal isolates, namely KBR1 and KBR8 (indigenous) and KBR1-1, KB4, KB2 and LB3 (exogenous). Based on the molecular and phylogenetic analysis, KBR1 and KB8 were identified as Aspergillus niger [MW699896] and tubingensis [MW699895], while KBR1-1, KB4, KB2 and LB3 were affiliated with the genera Syncephalastrum sp. [MZ817958], Paecilomyces formosus [MW699897], Fusarium chlamydosporum [MZ817957] and Coniochaeta sp. [MW699893], respectively. The highest rate of TPH degradation was recorded in soil microcosm treatments (SMT) after 60 days by inoculation with Paecilomyces formosus 97 ± 2.54%, followed by bioaugmentation with the native strain Aspergillus niger (92 ± 1.83%) and then by the fungal consortium (84 ± 2.21%). The statistical analysis of the results showed significant differences.
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spelling pubmed-102240322023-05-28 Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms Daâssi, Dalel Almaghrabi, Fatimah Qabil Microorganisms Article The main purpose of this study was to degrade total petroleum hydrocarbons (TPHs) from contaminated soil in batch microcosm reactors. Native soil fungi isolated from the same petroleum-polluted soil and ligninolytic fungal strains were screened and applied in the treatment of soil-contaminated microcosms in aerobic conditions. The bioaugmentation processes were carried out using selected hydrocarbonoclastic fungal strains in mono or co-cultures. Results demonstrated the petroleum-degrading potential of six fungal isolates, namely KBR1 and KBR8 (indigenous) and KBR1-1, KB4, KB2 and LB3 (exogenous). Based on the molecular and phylogenetic analysis, KBR1 and KB8 were identified as Aspergillus niger [MW699896] and tubingensis [MW699895], while KBR1-1, KB4, KB2 and LB3 were affiliated with the genera Syncephalastrum sp. [MZ817958], Paecilomyces formosus [MW699897], Fusarium chlamydosporum [MZ817957] and Coniochaeta sp. [MW699893], respectively. The highest rate of TPH degradation was recorded in soil microcosm treatments (SMT) after 60 days by inoculation with Paecilomyces formosus 97 ± 2.54%, followed by bioaugmentation with the native strain Aspergillus niger (92 ± 1.83%) and then by the fungal consortium (84 ± 2.21%). The statistical analysis of the results showed significant differences. MDPI 2023-05-22 /pmc/articles/PMC10224032/ /pubmed/37317325 http://dx.doi.org/10.3390/microorganisms11051351 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Daâssi, Dalel
Almaghrabi, Fatimah Qabil
Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms
title Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms
title_full Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms
title_fullStr Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms
title_full_unstemmed Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms
title_short Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms
title_sort petroleum-degrading fungal isolates for the treatment of soil microcosms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224032/
https://www.ncbi.nlm.nih.gov/pubmed/37317325
http://dx.doi.org/10.3390/microorganisms11051351
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