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CDH4 inhibits ferroptosis in oral squamous cell carcinoma cells

BACKGROUND: The cadherin-4 gene (CDH4), a member of the cadherin family genes, encodes R-cadherin (R-cad); however, the function of this gene in different types of cancer remains controversial. The function of CDH4 in OSCC (oral squamous cell carcinoma) is unknown. MATERIALS AND METHODS: We use the...

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Autores principales: Xie, Jian, Lan, Ting, Zheng, Da-Li, Ding, Lin-Can, Lu, You-Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224206/
https://www.ncbi.nlm.nih.gov/pubmed/37237299
http://dx.doi.org/10.1186/s12903-023-03046-3
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author Xie, Jian
Lan, Ting
Zheng, Da-Li
Ding, Lin-Can
Lu, You-Guang
author_facet Xie, Jian
Lan, Ting
Zheng, Da-Li
Ding, Lin-Can
Lu, You-Guang
author_sort Xie, Jian
collection PubMed
description BACKGROUND: The cadherin-4 gene (CDH4), a member of the cadherin family genes, encodes R-cadherin (R-cad); however, the function of this gene in different types of cancer remains controversial. The function of CDH4 in OSCC (oral squamous cell carcinoma) is unknown. MATERIALS AND METHODS: We use the Cancer Genome Atlas (TCGA) database to find the expression of CDH4 in OSCC is more than normal tissue. Our tissue samples also confirmed that CDH4 gene was highly expressed in OSCC. The related cell function assay detected that CDH4 promotes the ability of cell proliferation, migration, self-renewal and invasion. Cell staining experiment confirmed that the change of CDH4 expression would change the cell mortality. The western blot of GPX4 (glutathione-dependent peroxidase-4), GSH (reduced glutathione) test assay and MDA(Malondialdehyde) test assay show that the expression of CDH4 may resist the sensitivity of ferropotosis in OSCC. RESULTS: CDH4 was upregulated in OSCC samples and was correlation with poor survival of patients. High expression of CDH4 effectively promotes the proliferation, mobility of OSCC cells and reduce the sensitivity of OSCC cells to ferroptosis. CDH4 is positively correlated with EMT pathway genes, negatively correlated with fatty acid metabolism pathway genes and peroxisome pathway genes, and positively correlated with ferroptosis suppressor genes in OSCC. CONCLUSIONS: These results indicate that CDH4 may play a positive role in tumor progression and resistance ferroptosis and may be a potential therapeutic target for OSCC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12903-023-03046-3.
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spelling pubmed-102242062023-05-28 CDH4 inhibits ferroptosis in oral squamous cell carcinoma cells Xie, Jian Lan, Ting Zheng, Da-Li Ding, Lin-Can Lu, You-Guang BMC Oral Health Research BACKGROUND: The cadherin-4 gene (CDH4), a member of the cadherin family genes, encodes R-cadherin (R-cad); however, the function of this gene in different types of cancer remains controversial. The function of CDH4 in OSCC (oral squamous cell carcinoma) is unknown. MATERIALS AND METHODS: We use the Cancer Genome Atlas (TCGA) database to find the expression of CDH4 in OSCC is more than normal tissue. Our tissue samples also confirmed that CDH4 gene was highly expressed in OSCC. The related cell function assay detected that CDH4 promotes the ability of cell proliferation, migration, self-renewal and invasion. Cell staining experiment confirmed that the change of CDH4 expression would change the cell mortality. The western blot of GPX4 (glutathione-dependent peroxidase-4), GSH (reduced glutathione) test assay and MDA(Malondialdehyde) test assay show that the expression of CDH4 may resist the sensitivity of ferropotosis in OSCC. RESULTS: CDH4 was upregulated in OSCC samples and was correlation with poor survival of patients. High expression of CDH4 effectively promotes the proliferation, mobility of OSCC cells and reduce the sensitivity of OSCC cells to ferroptosis. CDH4 is positively correlated with EMT pathway genes, negatively correlated with fatty acid metabolism pathway genes and peroxisome pathway genes, and positively correlated with ferroptosis suppressor genes in OSCC. CONCLUSIONS: These results indicate that CDH4 may play a positive role in tumor progression and resistance ferroptosis and may be a potential therapeutic target for OSCC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12903-023-03046-3. BioMed Central 2023-05-27 /pmc/articles/PMC10224206/ /pubmed/37237299 http://dx.doi.org/10.1186/s12903-023-03046-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Xie, Jian
Lan, Ting
Zheng, Da-Li
Ding, Lin-Can
Lu, You-Guang
CDH4 inhibits ferroptosis in oral squamous cell carcinoma cells
title CDH4 inhibits ferroptosis in oral squamous cell carcinoma cells
title_full CDH4 inhibits ferroptosis in oral squamous cell carcinoma cells
title_fullStr CDH4 inhibits ferroptosis in oral squamous cell carcinoma cells
title_full_unstemmed CDH4 inhibits ferroptosis in oral squamous cell carcinoma cells
title_short CDH4 inhibits ferroptosis in oral squamous cell carcinoma cells
title_sort cdh4 inhibits ferroptosis in oral squamous cell carcinoma cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224206/
https://www.ncbi.nlm.nih.gov/pubmed/37237299
http://dx.doi.org/10.1186/s12903-023-03046-3
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