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Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus

Starting from October 2021, several outbreaks of highly pathogenic avian influenza virus (HPAIV) subtype H5N1 were reported in wild and domestic birds in Italy. Following the detection of an HPAIV in a free-ranging poultry farm in Ostia, province of Rome, despite the lack of clinical signs, addition...

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Autores principales: Rosone, Francesca, Bonfante, Francesco, Sala, Marcello Giovanni, Maniero, Silvia, Cersini, Antonella, Ricci, Ida, Garofalo, Luisa, Caciolo, Daniela, Denisi, Antonella, Napolitan, Alessandra, Parente, Monja, Zecchin, Bianca, Terregino, Calogero, Scicluna, Maria Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224318/
https://www.ncbi.nlm.nih.gov/pubmed/37317136
http://dx.doi.org/10.3390/microorganisms11051162
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author Rosone, Francesca
Bonfante, Francesco
Sala, Marcello Giovanni
Maniero, Silvia
Cersini, Antonella
Ricci, Ida
Garofalo, Luisa
Caciolo, Daniela
Denisi, Antonella
Napolitan, Alessandra
Parente, Monja
Zecchin, Bianca
Terregino, Calogero
Scicluna, Maria Teresa
author_facet Rosone, Francesca
Bonfante, Francesco
Sala, Marcello Giovanni
Maniero, Silvia
Cersini, Antonella
Ricci, Ida
Garofalo, Luisa
Caciolo, Daniela
Denisi, Antonella
Napolitan, Alessandra
Parente, Monja
Zecchin, Bianca
Terregino, Calogero
Scicluna, Maria Teresa
author_sort Rosone, Francesca
collection PubMed
description Starting from October 2021, several outbreaks of highly pathogenic avian influenza virus (HPAIV) subtype H5N1 were reported in wild and domestic birds in Italy. Following the detection of an HPAIV in a free-ranging poultry farm in Ostia, province of Rome, despite the lack of clinical signs, additional virological and serological analyses were conducted on samples collected from free-ranging pigs, reared in the same holding, due to their direct contact with the infected poultry. While the swine nasal swabs were all RT-PCR negative for the influenza type A matrix (M) gene, the majority (%) of the tested pigs resulted serologically positive for the hemagglutination inhibition test and microneutralization assay, using an H5N1 strain considered to be homologous to the virus detected in the farm. These results provide further evidence of the worrisome replicative fitness that HPAI H5Nx viruses of the 2.3.4.4b clade have in mammalian species. Moreover, our report calls for additional active surveillance, to promptly intercept occasional spillover transmissions to domestic mammals in close contact with HPAI affected birds. Strengthened biosecurity measures and efficient separation should be prioritized in mixed-species farms in areas at risk of HPAI introduction.
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spelling pubmed-102243182023-05-28 Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus Rosone, Francesca Bonfante, Francesco Sala, Marcello Giovanni Maniero, Silvia Cersini, Antonella Ricci, Ida Garofalo, Luisa Caciolo, Daniela Denisi, Antonella Napolitan, Alessandra Parente, Monja Zecchin, Bianca Terregino, Calogero Scicluna, Maria Teresa Microorganisms Article Starting from October 2021, several outbreaks of highly pathogenic avian influenza virus (HPAIV) subtype H5N1 were reported in wild and domestic birds in Italy. Following the detection of an HPAIV in a free-ranging poultry farm in Ostia, province of Rome, despite the lack of clinical signs, additional virological and serological analyses were conducted on samples collected from free-ranging pigs, reared in the same holding, due to their direct contact with the infected poultry. While the swine nasal swabs were all RT-PCR negative for the influenza type A matrix (M) gene, the majority (%) of the tested pigs resulted serologically positive for the hemagglutination inhibition test and microneutralization assay, using an H5N1 strain considered to be homologous to the virus detected in the farm. These results provide further evidence of the worrisome replicative fitness that HPAI H5Nx viruses of the 2.3.4.4b clade have in mammalian species. Moreover, our report calls for additional active surveillance, to promptly intercept occasional spillover transmissions to domestic mammals in close contact with HPAI affected birds. Strengthened biosecurity measures and efficient separation should be prioritized in mixed-species farms in areas at risk of HPAI introduction. MDPI 2023-04-28 /pmc/articles/PMC10224318/ /pubmed/37317136 http://dx.doi.org/10.3390/microorganisms11051162 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rosone, Francesca
Bonfante, Francesco
Sala, Marcello Giovanni
Maniero, Silvia
Cersini, Antonella
Ricci, Ida
Garofalo, Luisa
Caciolo, Daniela
Denisi, Antonella
Napolitan, Alessandra
Parente, Monja
Zecchin, Bianca
Terregino, Calogero
Scicluna, Maria Teresa
Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus
title Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus
title_full Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus
title_fullStr Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus
title_full_unstemmed Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus
title_short Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus
title_sort seroconversion of a swine herd in a free-range rural multi-species farm against hpai h5n1 2.3.4.4b clade virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224318/
https://www.ncbi.nlm.nih.gov/pubmed/37317136
http://dx.doi.org/10.3390/microorganisms11051162
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