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Characterization and Differentiation of Grain Proteomes from Wild-Type Puroindoline and Variants in Wheat

Premium wheat with a high end-use quality is generally lacking in China, especially high-quality hard and soft wheat. Pina-D1 and Pinb-D1 (puroindoline genes) influence wheat grain hardness (i.e., important wheat quality-related parameter) and are among the main targets in wheat breeding programs. H...

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Autores principales: Liu, Peixun, Liu, Zehou, Ma, Xiaofei, Wan, Hongshen, Zheng, Jianmin, Luo, Jiangtao, Deng, Qingyan, Mao, Qiang, Li, Xiaoye, Pu, Zongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224366/
https://www.ncbi.nlm.nih.gov/pubmed/37653896
http://dx.doi.org/10.3390/plants12101979
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author Liu, Peixun
Liu, Zehou
Ma, Xiaofei
Wan, Hongshen
Zheng, Jianmin
Luo, Jiangtao
Deng, Qingyan
Mao, Qiang
Li, Xiaoye
Pu, Zongjun
author_facet Liu, Peixun
Liu, Zehou
Ma, Xiaofei
Wan, Hongshen
Zheng, Jianmin
Luo, Jiangtao
Deng, Qingyan
Mao, Qiang
Li, Xiaoye
Pu, Zongjun
author_sort Liu, Peixun
collection PubMed
description Premium wheat with a high end-use quality is generally lacking in China, especially high-quality hard and soft wheat. Pina-D1 and Pinb-D1 (puroindoline genes) influence wheat grain hardness (i.e., important wheat quality-related parameter) and are among the main targets in wheat breeding programs. However, the mechanism by which puroindoline genes control grain hardness remains unclear. In this study, three hard wheat puroindoline variants (MY26, GX3, and ZM1) were compared with a soft wheat variety (CM605) containing the wild-type puroindoline genotype. Specifically, proteomic methods were used to screen for differentially abundant proteins (DAPs). In total, 6253 proteins were identified and quantified via a high-throughput tandem mass tag quantitative proteomic analysis. Of the 208 DAPs, 115, 116, and 99 proteins were differentially expressed between MY26, GX3, and ZM1 (hard wheat varieties) and CM605, respectively. The cluster analysis of protein relative abundances divided the proteins into six clusters. Of these proteins, 67 and 41 proteins were, respectively, more and less abundant in CM605 than in MY26, GX3, and ZM1. Enrichment analyses detected six GO terms, five KEGG pathways, and five IPR terms that were shared by all three comparisons. Furthermore, 12 proteins associated with these terms or pathways were found to be differentially expressed in each comparison. These proteins, which included cysteine proteinase inhibitors, invertases, low-molecular-weight glutenin subunits, and alpha amylase inhibitors, may be involved in the regulation of grain hardness. The candidate genes identified in this study may be relevant for future analyses of the regulatory mechanism underlying grain hardness.
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spelling pubmed-102243662023-05-28 Characterization and Differentiation of Grain Proteomes from Wild-Type Puroindoline and Variants in Wheat Liu, Peixun Liu, Zehou Ma, Xiaofei Wan, Hongshen Zheng, Jianmin Luo, Jiangtao Deng, Qingyan Mao, Qiang Li, Xiaoye Pu, Zongjun Plants (Basel) Article Premium wheat with a high end-use quality is generally lacking in China, especially high-quality hard and soft wheat. Pina-D1 and Pinb-D1 (puroindoline genes) influence wheat grain hardness (i.e., important wheat quality-related parameter) and are among the main targets in wheat breeding programs. However, the mechanism by which puroindoline genes control grain hardness remains unclear. In this study, three hard wheat puroindoline variants (MY26, GX3, and ZM1) were compared with a soft wheat variety (CM605) containing the wild-type puroindoline genotype. Specifically, proteomic methods were used to screen for differentially abundant proteins (DAPs). In total, 6253 proteins were identified and quantified via a high-throughput tandem mass tag quantitative proteomic analysis. Of the 208 DAPs, 115, 116, and 99 proteins were differentially expressed between MY26, GX3, and ZM1 (hard wheat varieties) and CM605, respectively. The cluster analysis of protein relative abundances divided the proteins into six clusters. Of these proteins, 67 and 41 proteins were, respectively, more and less abundant in CM605 than in MY26, GX3, and ZM1. Enrichment analyses detected six GO terms, five KEGG pathways, and five IPR terms that were shared by all three comparisons. Furthermore, 12 proteins associated with these terms or pathways were found to be differentially expressed in each comparison. These proteins, which included cysteine proteinase inhibitors, invertases, low-molecular-weight glutenin subunits, and alpha amylase inhibitors, may be involved in the regulation of grain hardness. The candidate genes identified in this study may be relevant for future analyses of the regulatory mechanism underlying grain hardness. MDPI 2023-05-15 /pmc/articles/PMC10224366/ /pubmed/37653896 http://dx.doi.org/10.3390/plants12101979 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Peixun
Liu, Zehou
Ma, Xiaofei
Wan, Hongshen
Zheng, Jianmin
Luo, Jiangtao
Deng, Qingyan
Mao, Qiang
Li, Xiaoye
Pu, Zongjun
Characterization and Differentiation of Grain Proteomes from Wild-Type Puroindoline and Variants in Wheat
title Characterization and Differentiation of Grain Proteomes from Wild-Type Puroindoline and Variants in Wheat
title_full Characterization and Differentiation of Grain Proteomes from Wild-Type Puroindoline and Variants in Wheat
title_fullStr Characterization and Differentiation of Grain Proteomes from Wild-Type Puroindoline and Variants in Wheat
title_full_unstemmed Characterization and Differentiation of Grain Proteomes from Wild-Type Puroindoline and Variants in Wheat
title_short Characterization and Differentiation of Grain Proteomes from Wild-Type Puroindoline and Variants in Wheat
title_sort characterization and differentiation of grain proteomes from wild-type puroindoline and variants in wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224366/
https://www.ncbi.nlm.nih.gov/pubmed/37653896
http://dx.doi.org/10.3390/plants12101979
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