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Comparative Study of Phytochemistry, Antioxidant and Biological Activities of Berberis libanotica Fruit and Leaf Extracts

Berberis libanotica Ehrenb. ex C.K. Schneider of the Berberidaceae family is an endemic Lebanese plant and is widely used in folk medicine. This study highlights the phytochemical composition and biological activities (in vitro) of fruit and leaf extracts. The two organs were extracted by cold macer...

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Autores principales: Dawra, Michella, Nehme, Nancy, El Beyrouthy, Marc, Abi Rizk, Alain, Taillandier, Patricia, Bouajila, Jalloul, El Rayess, Youssef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224532/
https://www.ncbi.nlm.nih.gov/pubmed/37653917
http://dx.doi.org/10.3390/plants12102001
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author Dawra, Michella
Nehme, Nancy
El Beyrouthy, Marc
Abi Rizk, Alain
Taillandier, Patricia
Bouajila, Jalloul
El Rayess, Youssef
author_facet Dawra, Michella
Nehme, Nancy
El Beyrouthy, Marc
Abi Rizk, Alain
Taillandier, Patricia
Bouajila, Jalloul
El Rayess, Youssef
author_sort Dawra, Michella
collection PubMed
description Berberis libanotica Ehrenb. ex C.K. Schneider of the Berberidaceae family is an endemic Lebanese plant and is widely used in folk medicine. This study highlights the phytochemical composition and biological activities (in vitro) of fruit and leaf extracts. The two organs were extracted by cold maceration with four solvents of increasing polarity: cyclohexane, dichloromethane, ethyl acetate and methanol. The extracts were screened for their chemical composition by HPLC-DAD to identify and quantify the phenolic compounds. Volatile compounds were detected by GC-MS. The antioxidant capacity through DPPH inhibition was tested. The anti-acetylcholinesterase, antibacterial and anti-proliferative activities were evaluated. Thirteen compounds, including 12 phenolics, were detected in the fruits, whereas 8 phenolic compounds were identified in the leaves. A total of 137 volatile compounds were identified in both organs. At 50 μg/mL, the methanolic leaf extract presented the highest antioxidant capacity, with an inhibition percentage of 54.9%. The dichloromethane fruit extract reduced the acetylcholinesterase activity by 65.3%. The cyclohexane leaf extract reduced the proliferation of the HCT-116 cells by 54.8%, while the dichloromethane fruit extract exhibited the best inhibition against the Caco-2 cells (54%). Interestingly, the minimum inhibitory concentration (MIC) value of the cyclohexane fruit extract against Salmonella enterica serovar Kentucky was 2.4 μg/mL, and the MIC value of the cyclohexane leaf extract against E. coli was 9.7 μg/mL.
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spelling pubmed-102245322023-05-28 Comparative Study of Phytochemistry, Antioxidant and Biological Activities of Berberis libanotica Fruit and Leaf Extracts Dawra, Michella Nehme, Nancy El Beyrouthy, Marc Abi Rizk, Alain Taillandier, Patricia Bouajila, Jalloul El Rayess, Youssef Plants (Basel) Article Berberis libanotica Ehrenb. ex C.K. Schneider of the Berberidaceae family is an endemic Lebanese plant and is widely used in folk medicine. This study highlights the phytochemical composition and biological activities (in vitro) of fruit and leaf extracts. The two organs were extracted by cold maceration with four solvents of increasing polarity: cyclohexane, dichloromethane, ethyl acetate and methanol. The extracts were screened for their chemical composition by HPLC-DAD to identify and quantify the phenolic compounds. Volatile compounds were detected by GC-MS. The antioxidant capacity through DPPH inhibition was tested. The anti-acetylcholinesterase, antibacterial and anti-proliferative activities were evaluated. Thirteen compounds, including 12 phenolics, were detected in the fruits, whereas 8 phenolic compounds were identified in the leaves. A total of 137 volatile compounds were identified in both organs. At 50 μg/mL, the methanolic leaf extract presented the highest antioxidant capacity, with an inhibition percentage of 54.9%. The dichloromethane fruit extract reduced the acetylcholinesterase activity by 65.3%. The cyclohexane leaf extract reduced the proliferation of the HCT-116 cells by 54.8%, while the dichloromethane fruit extract exhibited the best inhibition against the Caco-2 cells (54%). Interestingly, the minimum inhibitory concentration (MIC) value of the cyclohexane fruit extract against Salmonella enterica serovar Kentucky was 2.4 μg/mL, and the MIC value of the cyclohexane leaf extract against E. coli was 9.7 μg/mL. MDPI 2023-05-16 /pmc/articles/PMC10224532/ /pubmed/37653917 http://dx.doi.org/10.3390/plants12102001 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dawra, Michella
Nehme, Nancy
El Beyrouthy, Marc
Abi Rizk, Alain
Taillandier, Patricia
Bouajila, Jalloul
El Rayess, Youssef
Comparative Study of Phytochemistry, Antioxidant and Biological Activities of Berberis libanotica Fruit and Leaf Extracts
title Comparative Study of Phytochemistry, Antioxidant and Biological Activities of Berberis libanotica Fruit and Leaf Extracts
title_full Comparative Study of Phytochemistry, Antioxidant and Biological Activities of Berberis libanotica Fruit and Leaf Extracts
title_fullStr Comparative Study of Phytochemistry, Antioxidant and Biological Activities of Berberis libanotica Fruit and Leaf Extracts
title_full_unstemmed Comparative Study of Phytochemistry, Antioxidant and Biological Activities of Berberis libanotica Fruit and Leaf Extracts
title_short Comparative Study of Phytochemistry, Antioxidant and Biological Activities of Berberis libanotica Fruit and Leaf Extracts
title_sort comparative study of phytochemistry, antioxidant and biological activities of berberis libanotica fruit and leaf extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224532/
https://www.ncbi.nlm.nih.gov/pubmed/37653917
http://dx.doi.org/10.3390/plants12102001
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