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The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae Isochrysis galbana Based on the Transcriptome Analysis

The adverse effects of microplastics on microalgae species have been extensively studied, but their impact on the bait microalgae entering the food chain has not been well understood. This study investigated the cytological and physiological response of Isochrysis galbana to polyethylene microplasti...

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Autores principales: Xiao, Xinfeng, Li, Wenfang, Li, Shuangwei, Zuo, Xingsheng, Liu, Jie, Guo, Linke, Lu, Xiao, Zhang, Linlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224565/
https://www.ncbi.nlm.nih.gov/pubmed/37317083
http://dx.doi.org/10.3390/microorganisms11051108
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author Xiao, Xinfeng
Li, Wenfang
Li, Shuangwei
Zuo, Xingsheng
Liu, Jie
Guo, Linke
Lu, Xiao
Zhang, Linlin
author_facet Xiao, Xinfeng
Li, Wenfang
Li, Shuangwei
Zuo, Xingsheng
Liu, Jie
Guo, Linke
Lu, Xiao
Zhang, Linlin
author_sort Xiao, Xinfeng
collection PubMed
description The adverse effects of microplastics on microalgae species have been extensively studied, but their impact on the bait microalgae entering the food chain has not been well understood. This study investigated the cytological and physiological response of Isochrysis galbana to polyethylene microplastics (PE-MPs, 10 μm) and nanoplastics (PE-NPs, 50 nm). The results showed that PE-MPs had no significant impact on I. galbana, while PsE-NPs obviously inhibited cell growth, reduced chlorophyll content, and caused a decline in carotenoids and soluble protein. These changes in the quality of I. galbana could negatively affect its use as aquaculture feed. To understand the molecular response mechanism of I. galbana to PE-NPs, transcriptome sequencing was performed. The result revealed that the TCA cycle, purine metabolism, and some key amino acid syntheses were down-regulated by PE-NPs, while the Calvin cycle and fatty acid metabolism were up-regulated to tolerate PE-NP pressure. Microbial analysis showed that the bacterial community structure associated with I. galbana was significantly altered at the species level by PE-NPs. In conclusion, this study provides new insights into the physiological stress response caused by microplastic pollution based on transcriptome and bacterial community analysis. The findings highlight the need to mitigate the release of microplastics into the environment to prevent their harmful effects on aquatic ecosystems and will be helpful in understanding the impact of polyethylene nanoplastics on the bait microalgae.
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spelling pubmed-102245652023-05-28 The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae Isochrysis galbana Based on the Transcriptome Analysis Xiao, Xinfeng Li, Wenfang Li, Shuangwei Zuo, Xingsheng Liu, Jie Guo, Linke Lu, Xiao Zhang, Linlin Microorganisms Article The adverse effects of microplastics on microalgae species have been extensively studied, but their impact on the bait microalgae entering the food chain has not been well understood. This study investigated the cytological and physiological response of Isochrysis galbana to polyethylene microplastics (PE-MPs, 10 μm) and nanoplastics (PE-NPs, 50 nm). The results showed that PE-MPs had no significant impact on I. galbana, while PsE-NPs obviously inhibited cell growth, reduced chlorophyll content, and caused a decline in carotenoids and soluble protein. These changes in the quality of I. galbana could negatively affect its use as aquaculture feed. To understand the molecular response mechanism of I. galbana to PE-NPs, transcriptome sequencing was performed. The result revealed that the TCA cycle, purine metabolism, and some key amino acid syntheses were down-regulated by PE-NPs, while the Calvin cycle and fatty acid metabolism were up-regulated to tolerate PE-NP pressure. Microbial analysis showed that the bacterial community structure associated with I. galbana was significantly altered at the species level by PE-NPs. In conclusion, this study provides new insights into the physiological stress response caused by microplastic pollution based on transcriptome and bacterial community analysis. The findings highlight the need to mitigate the release of microplastics into the environment to prevent their harmful effects on aquatic ecosystems and will be helpful in understanding the impact of polyethylene nanoplastics on the bait microalgae. MDPI 2023-04-24 /pmc/articles/PMC10224565/ /pubmed/37317083 http://dx.doi.org/10.3390/microorganisms11051108 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiao, Xinfeng
Li, Wenfang
Li, Shuangwei
Zuo, Xingsheng
Liu, Jie
Guo, Linke
Lu, Xiao
Zhang, Linlin
The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae Isochrysis galbana Based on the Transcriptome Analysis
title The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae Isochrysis galbana Based on the Transcriptome Analysis
title_full The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae Isochrysis galbana Based on the Transcriptome Analysis
title_fullStr The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae Isochrysis galbana Based on the Transcriptome Analysis
title_full_unstemmed The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae Isochrysis galbana Based on the Transcriptome Analysis
title_short The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae Isochrysis galbana Based on the Transcriptome Analysis
title_sort growth inhibition of polyethylene nanoplastics on the bait-microalgae isochrysis galbana based on the transcriptome analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224565/
https://www.ncbi.nlm.nih.gov/pubmed/37317083
http://dx.doi.org/10.3390/microorganisms11051108
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