Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors

BACKGROUND: Conventional methods applied to develop recombinant CHO (rCHO) cell line as a predominant host for mammalian protein expression are limited to random integration approaches, which can prolong the process of getting the desired clones for months. CRISPR/Cas9 could be an alternative by med...

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Detalles Bibliográficos
Autores principales: Kheirandish, Mohammad Hassan, Rahmani, Behnaz, Zarei Jaliani, Hossein, Barkhordari, Farzaneh, Mazlomi, Mohammad Ali, Davami, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224643/
https://www.ncbi.nlm.nih.gov/pubmed/37244887
http://dx.doi.org/10.1007/s11033-023-08529-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224643/
https://www.ncbi.nlm.nih.gov/pubmed/37244887
http://dx.doi.org/10.1007/s11033-023-08529-8

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