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Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors
BACKGROUND: Conventional methods applied to develop recombinant CHO (rCHO) cell line as a predominant host for mammalian protein expression are limited to random integration approaches, which can prolong the process of getting the desired clones for months. CRISPR/Cas9 could be an alternative by med...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224643/ https://www.ncbi.nlm.nih.gov/pubmed/37244887 http://dx.doi.org/10.1007/s11033-023-08529-8 |