Cargando…

The Underlying Mechanism of Duanteng Yimu Decoction in Inhibiting Synovial Hyperplasia in Rheumatoid Arthritis

Dysregulation of microRNAs (miRNAs) is associated with the pathogenesis of rheumatoid arthritis (RA). Our previous studies confirmed that Duanteng Yimu decoction (DTYMT) effectively inhibits RA fibroblast-like synoviocyte (FLS) proliferation. In this study, we investigated the influence of DTYMT on...

Descripción completa

Detalles Bibliográficos
Autores principales: Feng, Wei, Zhong, Xiao-Qin, Zheng, Xue-Xia, Liu, Qing-Ping, Liu, Min-Ying, Liu, Xiao-Bao, Lin, Chang-Song, Xu, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225272/
https://www.ncbi.nlm.nih.gov/pubmed/37252680
http://dx.doi.org/10.1155/2023/2340538
_version_ 1785050365367943168
author Feng, Wei
Zhong, Xiao-Qin
Zheng, Xue-Xia
Liu, Qing-Ping
Liu, Min-Ying
Liu, Xiao-Bao
Lin, Chang-Song
Xu, Qiang
author_facet Feng, Wei
Zhong, Xiao-Qin
Zheng, Xue-Xia
Liu, Qing-Ping
Liu, Min-Ying
Liu, Xiao-Bao
Lin, Chang-Song
Xu, Qiang
author_sort Feng, Wei
collection PubMed
description Dysregulation of microRNAs (miRNAs) is associated with the pathogenesis of rheumatoid arthritis (RA). Our previous studies confirmed that Duanteng Yimu decoction (DTYMT) effectively inhibits RA fibroblast-like synoviocyte (FLS) proliferation. In this study, we investigated the influence of DTYMT on miR-221 in RA individuals. Hematoxylin–eosin (HE) staining was performed to assess histopathological alterations in collagen-induced arthritis (CIA) mice. The expression of miR-221-3p and TLR4 in PBMC, FLS, and cartilage was measured by RT-qPCR. In the in vitro experiments, DTYMT-containing serum was incubated with FLS-transfected miR-221 mimic or inhibitor. CCK-8 was performed to determine FLS proliferation, and the secretion of IL-1β, IL-6, IL-18, and TNF-α was quantified by ELISA assay. In addition, the regulation of miR-221 expression on FLS apoptosis was assessed using flow cytometry. Finally, western blot was employed to reflect TLR4/MyD88 protein levels. HE results showed that DTYMT effectively reduced synovial hyperplasia in the joints of CIA mice. RT-qPCR assay of FLS and cartilage of the model group showed that miR-221-3p and TLR4 significantly increased compared with those in the normal group. All outcomes were improved by DTYMT. The miR-221 mimic reversed the inhibitory effect of DTYMT-containing serum on FLS proliferation, the release of IL-1β, IL-18, IL-6, and TNF-α, and FLS apoptosis, as well as TLR4/MyD88 protein levels. The results showed that miR-221 promotes the activity of RA-FLS by activating TLR4/MyD88 signaling, and DTYMT treats RA by reducing miR-221 in CIA mice.
format Online
Article
Text
id pubmed-10225272
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-102252722023-05-29 The Underlying Mechanism of Duanteng Yimu Decoction in Inhibiting Synovial Hyperplasia in Rheumatoid Arthritis Feng, Wei Zhong, Xiao-Qin Zheng, Xue-Xia Liu, Qing-Ping Liu, Min-Ying Liu, Xiao-Bao Lin, Chang-Song Xu, Qiang J Immunol Res Research Article Dysregulation of microRNAs (miRNAs) is associated with the pathogenesis of rheumatoid arthritis (RA). Our previous studies confirmed that Duanteng Yimu decoction (DTYMT) effectively inhibits RA fibroblast-like synoviocyte (FLS) proliferation. In this study, we investigated the influence of DTYMT on miR-221 in RA individuals. Hematoxylin–eosin (HE) staining was performed to assess histopathological alterations in collagen-induced arthritis (CIA) mice. The expression of miR-221-3p and TLR4 in PBMC, FLS, and cartilage was measured by RT-qPCR. In the in vitro experiments, DTYMT-containing serum was incubated with FLS-transfected miR-221 mimic or inhibitor. CCK-8 was performed to determine FLS proliferation, and the secretion of IL-1β, IL-6, IL-18, and TNF-α was quantified by ELISA assay. In addition, the regulation of miR-221 expression on FLS apoptosis was assessed using flow cytometry. Finally, western blot was employed to reflect TLR4/MyD88 protein levels. HE results showed that DTYMT effectively reduced synovial hyperplasia in the joints of CIA mice. RT-qPCR assay of FLS and cartilage of the model group showed that miR-221-3p and TLR4 significantly increased compared with those in the normal group. All outcomes were improved by DTYMT. The miR-221 mimic reversed the inhibitory effect of DTYMT-containing serum on FLS proliferation, the release of IL-1β, IL-18, IL-6, and TNF-α, and FLS apoptosis, as well as TLR4/MyD88 protein levels. The results showed that miR-221 promotes the activity of RA-FLS by activating TLR4/MyD88 signaling, and DTYMT treats RA by reducing miR-221 in CIA mice. Hindawi 2023-05-21 /pmc/articles/PMC10225272/ /pubmed/37252680 http://dx.doi.org/10.1155/2023/2340538 Text en Copyright © 2023 Wei Feng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Feng, Wei
Zhong, Xiao-Qin
Zheng, Xue-Xia
Liu, Qing-Ping
Liu, Min-Ying
Liu, Xiao-Bao
Lin, Chang-Song
Xu, Qiang
The Underlying Mechanism of Duanteng Yimu Decoction in Inhibiting Synovial Hyperplasia in Rheumatoid Arthritis
title The Underlying Mechanism of Duanteng Yimu Decoction in Inhibiting Synovial Hyperplasia in Rheumatoid Arthritis
title_full The Underlying Mechanism of Duanteng Yimu Decoction in Inhibiting Synovial Hyperplasia in Rheumatoid Arthritis
title_fullStr The Underlying Mechanism of Duanteng Yimu Decoction in Inhibiting Synovial Hyperplasia in Rheumatoid Arthritis
title_full_unstemmed The Underlying Mechanism of Duanteng Yimu Decoction in Inhibiting Synovial Hyperplasia in Rheumatoid Arthritis
title_short The Underlying Mechanism of Duanteng Yimu Decoction in Inhibiting Synovial Hyperplasia in Rheumatoid Arthritis
title_sort underlying mechanism of duanteng yimu decoction in inhibiting synovial hyperplasia in rheumatoid arthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225272/
https://www.ncbi.nlm.nih.gov/pubmed/37252680
http://dx.doi.org/10.1155/2023/2340538
work_keys_str_mv AT fengwei theunderlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT zhongxiaoqin theunderlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT zhengxuexia theunderlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT liuqingping theunderlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT liuminying theunderlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT liuxiaobao theunderlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT linchangsong theunderlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT xuqiang theunderlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT fengwei underlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT zhongxiaoqin underlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT zhengxuexia underlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT liuqingping underlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT liuminying underlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT liuxiaobao underlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT linchangsong underlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis
AT xuqiang underlyingmechanismofduantengyimudecoctionininhibitingsynovialhyperplasiainrheumatoidarthritis