Cargando…

BDNF and KISS-1 Levels in Maternal Serum, Umbilical Cord, and Placenta: The Potential Role of Maternal Levels as Effect Biomarker

Brain-derived neurotrophic factor (BDNF) and kisspeptin-1 (KISS-1) regulate placental development and fetal growth. The predictive value of maternal serum BDNF and KISS-1 concentrations for placental and umbilical cord levels has not yet been explored. The influence of prenatal lead (Pb) and cadmium...

Descripción completa

Detalles Bibliográficos
Autores principales: Granitzer, Sebastian, Widhalm, Raimund, Atteneder, Simon, Fernandez, Mariana F., Mustieles, Vicente, Zeisler, Harald, Hengstschläger, Markus, Gundacker, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225291/
https://www.ncbi.nlm.nih.gov/pubmed/37360514
http://dx.doi.org/10.1007/s12403-023-00565-w
_version_ 1785050369808662528
author Granitzer, Sebastian
Widhalm, Raimund
Atteneder, Simon
Fernandez, Mariana F.
Mustieles, Vicente
Zeisler, Harald
Hengstschläger, Markus
Gundacker, Claudia
author_facet Granitzer, Sebastian
Widhalm, Raimund
Atteneder, Simon
Fernandez, Mariana F.
Mustieles, Vicente
Zeisler, Harald
Hengstschläger, Markus
Gundacker, Claudia
author_sort Granitzer, Sebastian
collection PubMed
description Brain-derived neurotrophic factor (BDNF) and kisspeptin-1 (KISS-1) regulate placental development and fetal growth. The predictive value of maternal serum BDNF and KISS-1 concentrations for placental and umbilical cord levels has not yet been explored. The influence of prenatal lead (Pb) and cadmium (Cd) exposure and maternal iron status on BDNF and KISS-1 levels is also unclarified and of concern. In a pilot cross-sectional study with 65 mother–newborn pairs, we analyzed maternal and cord serum levels of pro-BDNF, mature BDNF, and KISS-1, BDNF, and KISS-1 gene expression in placenta, Pb and Cd in maternal and umbilical cord blood (erythrocytes), and placenta. We conducted a series of in vitro experiments using human primary trophoblast cells (hTCs) and BeWo cells to verify main findings of the epidemiological analysis. Strong and consistent correlations were observed between maternal serum levels of pro-BDNF, mature BDNF, and KISS-1 and corresponding levels in umbilical serum and placental tissue. Maternal red blood cell Pb levels were inversely correlated with serum and placental KISS-1 levels. Lower expression and release of KISS-1 was also observed in Pb-exposed BeWo cells. In vitro Pb exposure also reduced cellular BDNF levels. Cd-treated BeWo cells showed increased pro-BDNF levels. Low maternal iron status was positively associated with low BDNF levels. Iron-deficient hTCs and BeWo cells showed a consistent decrease in the release of mature BDNF. The correlations between maternal BDNF and KISS-1 levels, placental gene expression, and umbilical cord serum levels, respectively, indicate the strong potential of maternal serum as predictive matrix for BDNF and KISS-1 levels in placentas and fetal sera. Pb exposure and iron status modulate BDNF and KISS-1 levels, but a clear direction of modulations was not evident. The associations need to be confirmed in a larger sample and validated in terms of placental and neurodevelopmental function. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12403-023-00565-w.
format Online
Article
Text
id pubmed-10225291
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-102252912023-05-30 BDNF and KISS-1 Levels in Maternal Serum, Umbilical Cord, and Placenta: The Potential Role of Maternal Levels as Effect Biomarker Granitzer, Sebastian Widhalm, Raimund Atteneder, Simon Fernandez, Mariana F. Mustieles, Vicente Zeisler, Harald Hengstschläger, Markus Gundacker, Claudia Expo Health Original Paper Brain-derived neurotrophic factor (BDNF) and kisspeptin-1 (KISS-1) regulate placental development and fetal growth. The predictive value of maternal serum BDNF and KISS-1 concentrations for placental and umbilical cord levels has not yet been explored. The influence of prenatal lead (Pb) and cadmium (Cd) exposure and maternal iron status on BDNF and KISS-1 levels is also unclarified and of concern. In a pilot cross-sectional study with 65 mother–newborn pairs, we analyzed maternal and cord serum levels of pro-BDNF, mature BDNF, and KISS-1, BDNF, and KISS-1 gene expression in placenta, Pb and Cd in maternal and umbilical cord blood (erythrocytes), and placenta. We conducted a series of in vitro experiments using human primary trophoblast cells (hTCs) and BeWo cells to verify main findings of the epidemiological analysis. Strong and consistent correlations were observed between maternal serum levels of pro-BDNF, mature BDNF, and KISS-1 and corresponding levels in umbilical serum and placental tissue. Maternal red blood cell Pb levels were inversely correlated with serum and placental KISS-1 levels. Lower expression and release of KISS-1 was also observed in Pb-exposed BeWo cells. In vitro Pb exposure also reduced cellular BDNF levels. Cd-treated BeWo cells showed increased pro-BDNF levels. Low maternal iron status was positively associated with low BDNF levels. Iron-deficient hTCs and BeWo cells showed a consistent decrease in the release of mature BDNF. The correlations between maternal BDNF and KISS-1 levels, placental gene expression, and umbilical cord serum levels, respectively, indicate the strong potential of maternal serum as predictive matrix for BDNF and KISS-1 levels in placentas and fetal sera. Pb exposure and iron status modulate BDNF and KISS-1 levels, but a clear direction of modulations was not evident. The associations need to be confirmed in a larger sample and validated in terms of placental and neurodevelopmental function. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12403-023-00565-w. Springer Netherlands 2023-05-29 /pmc/articles/PMC10225291/ /pubmed/37360514 http://dx.doi.org/10.1007/s12403-023-00565-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Granitzer, Sebastian
Widhalm, Raimund
Atteneder, Simon
Fernandez, Mariana F.
Mustieles, Vicente
Zeisler, Harald
Hengstschläger, Markus
Gundacker, Claudia
BDNF and KISS-1 Levels in Maternal Serum, Umbilical Cord, and Placenta: The Potential Role of Maternal Levels as Effect Biomarker
title BDNF and KISS-1 Levels in Maternal Serum, Umbilical Cord, and Placenta: The Potential Role of Maternal Levels as Effect Biomarker
title_full BDNF and KISS-1 Levels in Maternal Serum, Umbilical Cord, and Placenta: The Potential Role of Maternal Levels as Effect Biomarker
title_fullStr BDNF and KISS-1 Levels in Maternal Serum, Umbilical Cord, and Placenta: The Potential Role of Maternal Levels as Effect Biomarker
title_full_unstemmed BDNF and KISS-1 Levels in Maternal Serum, Umbilical Cord, and Placenta: The Potential Role of Maternal Levels as Effect Biomarker
title_short BDNF and KISS-1 Levels in Maternal Serum, Umbilical Cord, and Placenta: The Potential Role of Maternal Levels as Effect Biomarker
title_sort bdnf and kiss-1 levels in maternal serum, umbilical cord, and placenta: the potential role of maternal levels as effect biomarker
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225291/
https://www.ncbi.nlm.nih.gov/pubmed/37360514
http://dx.doi.org/10.1007/s12403-023-00565-w
work_keys_str_mv AT granitzersebastian bdnfandkiss1levelsinmaternalserumumbilicalcordandplacentathepotentialroleofmaternallevelsaseffectbiomarker
AT widhalmraimund bdnfandkiss1levelsinmaternalserumumbilicalcordandplacentathepotentialroleofmaternallevelsaseffectbiomarker
AT attenedersimon bdnfandkiss1levelsinmaternalserumumbilicalcordandplacentathepotentialroleofmaternallevelsaseffectbiomarker
AT fernandezmarianaf bdnfandkiss1levelsinmaternalserumumbilicalcordandplacentathepotentialroleofmaternallevelsaseffectbiomarker
AT mustielesvicente bdnfandkiss1levelsinmaternalserumumbilicalcordandplacentathepotentialroleofmaternallevelsaseffectbiomarker
AT zeislerharald bdnfandkiss1levelsinmaternalserumumbilicalcordandplacentathepotentialroleofmaternallevelsaseffectbiomarker
AT hengstschlagermarkus bdnfandkiss1levelsinmaternalserumumbilicalcordandplacentathepotentialroleofmaternallevelsaseffectbiomarker
AT gundackerclaudia bdnfandkiss1levelsinmaternalserumumbilicalcordandplacentathepotentialroleofmaternallevelsaseffectbiomarker