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Periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors in Pseudomonas aeruginosa

Pseudomonas aeruginosa uses quorum sensing to regulate the expression of virulence factors. In static environments, spatial structures, such as biofilms, can increase the expression of these virulence factors. However, in natural settings, biofilms are exposed to physical forces that disrupt spatial...

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Autores principales: García-Diéguez, Laura, Diaz-Tang, Gabriela, Marin Meneses, Estefania, Cruise, Vanessa, Barraza, Ivana, Craddock, Travis J.A., Smith, Robert P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225924/
https://www.ncbi.nlm.nih.gov/pubmed/37255658
http://dx.doi.org/10.1016/j.isci.2023.106843
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author García-Diéguez, Laura
Diaz-Tang, Gabriela
Marin Meneses, Estefania
Cruise, Vanessa
Barraza, Ivana
Craddock, Travis J.A.
Smith, Robert P.
author_facet García-Diéguez, Laura
Diaz-Tang, Gabriela
Marin Meneses, Estefania
Cruise, Vanessa
Barraza, Ivana
Craddock, Travis J.A.
Smith, Robert P.
author_sort García-Diéguez, Laura
collection PubMed
description Pseudomonas aeruginosa uses quorum sensing to regulate the expression of virulence factors. In static environments, spatial structures, such as biofilms, can increase the expression of these virulence factors. However, in natural settings, biofilms are exposed to physical forces that disrupt spatial structure, which may affect the expression of virulence factors regulated by quorum sensing. We show that periodically disturbing biofilms composed of P. aeruginosa using a physical force reduces the expression of quorum sensing-regulated virulence factors. At an intermediate disturbance frequency, the expression of virulence factors in the las, rhl, and pqs regulons is reduced. Mathematical modeling suggests that perturbation of the pqsR receptor is critical for this reduction. Removing the lasR receptor enhances the reduction in the expression of virulence factors as a result of disturbance. Our results allow identification of environments where virulence is reduced and implicate the lasR receptor as having a buffering role against disturbance.
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spelling pubmed-102259242023-05-30 Periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors in Pseudomonas aeruginosa García-Diéguez, Laura Diaz-Tang, Gabriela Marin Meneses, Estefania Cruise, Vanessa Barraza, Ivana Craddock, Travis J.A. Smith, Robert P. iScience Article Pseudomonas aeruginosa uses quorum sensing to regulate the expression of virulence factors. In static environments, spatial structures, such as biofilms, can increase the expression of these virulence factors. However, in natural settings, biofilms are exposed to physical forces that disrupt spatial structure, which may affect the expression of virulence factors regulated by quorum sensing. We show that periodically disturbing biofilms composed of P. aeruginosa using a physical force reduces the expression of quorum sensing-regulated virulence factors. At an intermediate disturbance frequency, the expression of virulence factors in the las, rhl, and pqs regulons is reduced. Mathematical modeling suggests that perturbation of the pqsR receptor is critical for this reduction. Removing the lasR receptor enhances the reduction in the expression of virulence factors as a result of disturbance. Our results allow identification of environments where virulence is reduced and implicate the lasR receptor as having a buffering role against disturbance. Elsevier 2023-05-11 /pmc/articles/PMC10225924/ /pubmed/37255658 http://dx.doi.org/10.1016/j.isci.2023.106843 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
García-Diéguez, Laura
Diaz-Tang, Gabriela
Marin Meneses, Estefania
Cruise, Vanessa
Barraza, Ivana
Craddock, Travis J.A.
Smith, Robert P.
Periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors in Pseudomonas aeruginosa
title Periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors in Pseudomonas aeruginosa
title_full Periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors in Pseudomonas aeruginosa
title_fullStr Periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors in Pseudomonas aeruginosa
title_full_unstemmed Periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors in Pseudomonas aeruginosa
title_short Periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors in Pseudomonas aeruginosa
title_sort periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors in pseudomonas aeruginosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225924/
https://www.ncbi.nlm.nih.gov/pubmed/37255658
http://dx.doi.org/10.1016/j.isci.2023.106843
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