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Quinolinium-Based Fluorescent Probes for Dynamic pH Monitoring in Aqueous Media at High pH Using Fluorescence Lifetime Imaging
[Image: see text] Spatiotemporal pH imaging using fluorescence lifetime imaging microscopy (FLIM) is an excellent technique for investigating dynamic (electro)chemical processes. However, probes that are responsive at high pH values are not available. Here, we describe the development and applicatio...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226165/ https://www.ncbi.nlm.nih.gov/pubmed/37128994 http://dx.doi.org/10.1021/acssensors.3c00316 |
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author | Bleeker, Jorrit Kahn, Aron P. Baumgartner, Lorenz M. Grozema, Ferdinand C. Vermaas, David A. Jager, Wolter F. |
author_facet | Bleeker, Jorrit Kahn, Aron P. Baumgartner, Lorenz M. Grozema, Ferdinand C. Vermaas, David A. Jager, Wolter F. |
author_sort | Bleeker, Jorrit |
collection | PubMed |
description | [Image: see text] Spatiotemporal pH imaging using fluorescence lifetime imaging microscopy (FLIM) is an excellent technique for investigating dynamic (electro)chemical processes. However, probes that are responsive at high pH values are not available. Here, we describe the development and application of dedicated pH probes based on the 1-methyl-7-amino-quinolinium fluorophore. The high fluorescence lifetime and quantum yield, the high (photo)stability, and the inherent water solubility make the quinolinium fluorophore well suited for the development of FLIM probes. Due to the flexible fluorophore-spacer–receptor architecture, probe lifetimes are tunable in the pH range between 5.5 and 11. An additional fluorescence lifetime response, at tunable pH values between 11 and 13, is achieved by deprotonation of the aromatic amine at the quinolinium core. Probe lifetimes are hardly affected by temperature and the presence of most inorganic ions, thus making FLIM imaging highly reliable and convenient. At 0.1 mM probe concentrations, imaging at rates of 3 images per second, at a resolution of 4 μm, while measuring pH values up to 12 is achieved. This enables the pH imaging of dynamic electrochemical processes involving chemical reactions and mass transport. |
format | Online Article Text |
id | pubmed-10226165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-102261652023-05-30 Quinolinium-Based Fluorescent Probes for Dynamic pH Monitoring in Aqueous Media at High pH Using Fluorescence Lifetime Imaging Bleeker, Jorrit Kahn, Aron P. Baumgartner, Lorenz M. Grozema, Ferdinand C. Vermaas, David A. Jager, Wolter F. ACS Sens [Image: see text] Spatiotemporal pH imaging using fluorescence lifetime imaging microscopy (FLIM) is an excellent technique for investigating dynamic (electro)chemical processes. However, probes that are responsive at high pH values are not available. Here, we describe the development and application of dedicated pH probes based on the 1-methyl-7-amino-quinolinium fluorophore. The high fluorescence lifetime and quantum yield, the high (photo)stability, and the inherent water solubility make the quinolinium fluorophore well suited for the development of FLIM probes. Due to the flexible fluorophore-spacer–receptor architecture, probe lifetimes are tunable in the pH range between 5.5 and 11. An additional fluorescence lifetime response, at tunable pH values between 11 and 13, is achieved by deprotonation of the aromatic amine at the quinolinium core. Probe lifetimes are hardly affected by temperature and the presence of most inorganic ions, thus making FLIM imaging highly reliable and convenient. At 0.1 mM probe concentrations, imaging at rates of 3 images per second, at a resolution of 4 μm, while measuring pH values up to 12 is achieved. This enables the pH imaging of dynamic electrochemical processes involving chemical reactions and mass transport. American Chemical Society 2023-04-27 /pmc/articles/PMC10226165/ /pubmed/37128994 http://dx.doi.org/10.1021/acssensors.3c00316 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Bleeker, Jorrit Kahn, Aron P. Baumgartner, Lorenz M. Grozema, Ferdinand C. Vermaas, David A. Jager, Wolter F. Quinolinium-Based Fluorescent Probes for Dynamic pH Monitoring in Aqueous Media at High pH Using Fluorescence Lifetime Imaging |
title | Quinolinium-Based
Fluorescent Probes for Dynamic pH
Monitoring in Aqueous Media at High pH Using Fluorescence Lifetime
Imaging |
title_full | Quinolinium-Based
Fluorescent Probes for Dynamic pH
Monitoring in Aqueous Media at High pH Using Fluorescence Lifetime
Imaging |
title_fullStr | Quinolinium-Based
Fluorescent Probes for Dynamic pH
Monitoring in Aqueous Media at High pH Using Fluorescence Lifetime
Imaging |
title_full_unstemmed | Quinolinium-Based
Fluorescent Probes for Dynamic pH
Monitoring in Aqueous Media at High pH Using Fluorescence Lifetime
Imaging |
title_short | Quinolinium-Based
Fluorescent Probes for Dynamic pH
Monitoring in Aqueous Media at High pH Using Fluorescence Lifetime
Imaging |
title_sort | quinolinium-based
fluorescent probes for dynamic ph
monitoring in aqueous media at high ph using fluorescence lifetime
imaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226165/ https://www.ncbi.nlm.nih.gov/pubmed/37128994 http://dx.doi.org/10.1021/acssensors.3c00316 |
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