ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells

BACKGROUND: Recent advances in CRISPR technology have enabled us to perform gene knock-in in various species and cell lines. CRISPR-mediated knock-in requires donor DNA which serves as a template for homology-directed repair (HDR). For knock-in of short sequences or base substitutions, ssDNA donors...

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Detalles Bibliográficos
Autores principales: Mabuchi, Akira, Hata, Shoji, Genova, Mariya, Tei, Chiharu, Ito, Kei K., Hirota, Masayasu, Komori, Takuma, Fukuyama, Masamitsu, Chinen, Takumi, Toyoda, Atsushi, Kitagawa, Daiju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226222/
https://www.ncbi.nlm.nih.gov/pubmed/37248464
http://dx.doi.org/10.1186/s12864-023-09377-3

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