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Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology
Eleutherine bulbosa bulbs, an endemic plant in Indonesia, have enormous potential as raw materials for pharmaceutical products. Therefore, it is necessary to strengthen and develop extraction methods that are easy, rapid, and efficient to enrich targeted secondary metabolites. This study aims to opt...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226706/ https://www.ncbi.nlm.nih.gov/pubmed/37255875 http://dx.doi.org/10.4103/japtr.japtr_613_22 |
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author | Ahmad, Islamudin Narsa, Angga Cipta Ramadhani, M. Riki Zamruddin, Nur Masyithah Iswahyudi, Iswahyudi Hajrah, Hajrah Indriyanti, Niken Arifuddin, M. Siska, Siska Supandi, Supandi Ambarwati, Neneng Siti Silfi |
author_facet | Ahmad, Islamudin Narsa, Angga Cipta Ramadhani, M. Riki Zamruddin, Nur Masyithah Iswahyudi, Iswahyudi Hajrah, Hajrah Indriyanti, Niken Arifuddin, M. Siska, Siska Supandi, Supandi Ambarwati, Neneng Siti Silfi |
author_sort | Ahmad, Islamudin |
collection | PubMed |
description | Eleutherine bulbosa bulbs, an endemic plant in Indonesia, have enormous potential as raw materials for pharmaceutical products. Therefore, it is necessary to strengthen and develop extraction methods that are easy, rapid, and efficient to enrich targeted secondary metabolites. This study aims to optimize the microwave-assisted extraction (MAE) method conditions for polyphenol metabolite from E. bulbosa bulbs. The MAE method (with different conditions) was applied to extract total polyphenol content (TPC) from E. bulbosa bulbs. TPC values were determined using a 96-well microplate reader spectrophotometry method and Folin–Ciocalteu reagent. The variables of MAE, as an experimental design-independent variable, were involved. The MAE method condition was optimized using response surface methodology (RSM) and Box–Behnken design based on the TPC value. The MAE condition was optimized with 60% ethanol, sample–solvent ratio of 1:10 g/mL, and 50% Watts of microwave power for 10 min. The quadratic regression analysis was achieved to predict the TPC value using the equation: TPC value = 28.63–5.545A +2.211B –0.741C +1.995D – 4.045AB +0.856AC –7.541BC +1.961CD –8.342A(2)–0.071B(2) +1.840C(2)–1.535D(2). For the scale-up confirmation test, a 50-g sample was used to prove the validity of the equation to predict the TPC value, yielding 35.33 ± 2.13 mg gallic acid equivalent/g samples. The optimum of the MAE condition recommended based on the results of RSM analysis can be applied directly to the enrichment of polyphenols metabolite constituent of E. bulbosa easily, cheaply, quickly, and efficiently. |
format | Online Article Text |
id | pubmed-10226706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-102267062023-05-30 Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology Ahmad, Islamudin Narsa, Angga Cipta Ramadhani, M. Riki Zamruddin, Nur Masyithah Iswahyudi, Iswahyudi Hajrah, Hajrah Indriyanti, Niken Arifuddin, M. Siska, Siska Supandi, Supandi Ambarwati, Neneng Siti Silfi J Adv Pharm Technol Res Original Article Eleutherine bulbosa bulbs, an endemic plant in Indonesia, have enormous potential as raw materials for pharmaceutical products. Therefore, it is necessary to strengthen and develop extraction methods that are easy, rapid, and efficient to enrich targeted secondary metabolites. This study aims to optimize the microwave-assisted extraction (MAE) method conditions for polyphenol metabolite from E. bulbosa bulbs. The MAE method (with different conditions) was applied to extract total polyphenol content (TPC) from E. bulbosa bulbs. TPC values were determined using a 96-well microplate reader spectrophotometry method and Folin–Ciocalteu reagent. The variables of MAE, as an experimental design-independent variable, were involved. The MAE method condition was optimized using response surface methodology (RSM) and Box–Behnken design based on the TPC value. The MAE condition was optimized with 60% ethanol, sample–solvent ratio of 1:10 g/mL, and 50% Watts of microwave power for 10 min. The quadratic regression analysis was achieved to predict the TPC value using the equation: TPC value = 28.63–5.545A +2.211B –0.741C +1.995D – 4.045AB +0.856AC –7.541BC +1.961CD –8.342A(2)–0.071B(2) +1.840C(2)–1.535D(2). For the scale-up confirmation test, a 50-g sample was used to prove the validity of the equation to predict the TPC value, yielding 35.33 ± 2.13 mg gallic acid equivalent/g samples. The optimum of the MAE condition recommended based on the results of RSM analysis can be applied directly to the enrichment of polyphenols metabolite constituent of E. bulbosa easily, cheaply, quickly, and efficiently. Wolters Kluwer - Medknow 2023 2023-04-13 /pmc/articles/PMC10226706/ /pubmed/37255875 http://dx.doi.org/10.4103/japtr.japtr_613_22 Text en Copyright: © 2023 Journal of Advanced Pharmaceutical Technology & Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Ahmad, Islamudin Narsa, Angga Cipta Ramadhani, M. Riki Zamruddin, Nur Masyithah Iswahyudi, Iswahyudi Hajrah, Hajrah Indriyanti, Niken Arifuddin, M. Siska, Siska Supandi, Supandi Ambarwati, Neneng Siti Silfi Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology |
title | Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology |
title_full | Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology |
title_fullStr | Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology |
title_full_unstemmed | Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology |
title_short | Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology |
title_sort | optimization of microwave-assisted extraction on polyphenol metabolite from eleutherine bulbosa (mill.) urb. bulbs using response surface methodology |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226706/ https://www.ncbi.nlm.nih.gov/pubmed/37255875 http://dx.doi.org/10.4103/japtr.japtr_613_22 |
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