Cargando…

Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology

Eleutherine bulbosa bulbs, an endemic plant in Indonesia, have enormous potential as raw materials for pharmaceutical products. Therefore, it is necessary to strengthen and develop extraction methods that are easy, rapid, and efficient to enrich targeted secondary metabolites. This study aims to opt...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahmad, Islamudin, Narsa, Angga Cipta, Ramadhani, M. Riki, Zamruddin, Nur Masyithah, Iswahyudi, Iswahyudi, Hajrah, Hajrah, Indriyanti, Niken, Arifuddin, M., Siska, Siska, Supandi, Supandi, Ambarwati, Neneng Siti Silfi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226706/
https://www.ncbi.nlm.nih.gov/pubmed/37255875
http://dx.doi.org/10.4103/japtr.japtr_613_22
_version_ 1785050628923326464
author Ahmad, Islamudin
Narsa, Angga Cipta
Ramadhani, M. Riki
Zamruddin, Nur Masyithah
Iswahyudi, Iswahyudi
Hajrah, Hajrah
Indriyanti, Niken
Arifuddin, M.
Siska, Siska
Supandi, Supandi
Ambarwati, Neneng Siti Silfi
author_facet Ahmad, Islamudin
Narsa, Angga Cipta
Ramadhani, M. Riki
Zamruddin, Nur Masyithah
Iswahyudi, Iswahyudi
Hajrah, Hajrah
Indriyanti, Niken
Arifuddin, M.
Siska, Siska
Supandi, Supandi
Ambarwati, Neneng Siti Silfi
author_sort Ahmad, Islamudin
collection PubMed
description Eleutherine bulbosa bulbs, an endemic plant in Indonesia, have enormous potential as raw materials for pharmaceutical products. Therefore, it is necessary to strengthen and develop extraction methods that are easy, rapid, and efficient to enrich targeted secondary metabolites. This study aims to optimize the microwave-assisted extraction (MAE) method conditions for polyphenol metabolite from E. bulbosa bulbs. The MAE method (with different conditions) was applied to extract total polyphenol content (TPC) from E. bulbosa bulbs. TPC values were determined using a 96-well microplate reader spectrophotometry method and Folin–Ciocalteu reagent. The variables of MAE, as an experimental design-independent variable, were involved. The MAE method condition was optimized using response surface methodology (RSM) and Box–Behnken design based on the TPC value. The MAE condition was optimized with 60% ethanol, sample–solvent ratio of 1:10 g/mL, and 50% Watts of microwave power for 10 min. The quadratic regression analysis was achieved to predict the TPC value using the equation: TPC value = 28.63–5.545A +2.211B –0.741C +1.995D – 4.045AB +0.856AC –7.541BC +1.961CD –8.342A(2)–0.071B(2) +1.840C(2)–1.535D(2). For the scale-up confirmation test, a 50-g sample was used to prove the validity of the equation to predict the TPC value, yielding 35.33 ± 2.13 mg gallic acid equivalent/g samples. The optimum of the MAE condition recommended based on the results of RSM analysis can be applied directly to the enrichment of polyphenols metabolite constituent of E. bulbosa easily, cheaply, quickly, and efficiently.
format Online
Article
Text
id pubmed-10226706
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Wolters Kluwer - Medknow
record_format MEDLINE/PubMed
spelling pubmed-102267062023-05-30 Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology Ahmad, Islamudin Narsa, Angga Cipta Ramadhani, M. Riki Zamruddin, Nur Masyithah Iswahyudi, Iswahyudi Hajrah, Hajrah Indriyanti, Niken Arifuddin, M. Siska, Siska Supandi, Supandi Ambarwati, Neneng Siti Silfi J Adv Pharm Technol Res Original Article Eleutherine bulbosa bulbs, an endemic plant in Indonesia, have enormous potential as raw materials for pharmaceutical products. Therefore, it is necessary to strengthen and develop extraction methods that are easy, rapid, and efficient to enrich targeted secondary metabolites. This study aims to optimize the microwave-assisted extraction (MAE) method conditions for polyphenol metabolite from E. bulbosa bulbs. The MAE method (with different conditions) was applied to extract total polyphenol content (TPC) from E. bulbosa bulbs. TPC values were determined using a 96-well microplate reader spectrophotometry method and Folin–Ciocalteu reagent. The variables of MAE, as an experimental design-independent variable, were involved. The MAE method condition was optimized using response surface methodology (RSM) and Box–Behnken design based on the TPC value. The MAE condition was optimized with 60% ethanol, sample–solvent ratio of 1:10 g/mL, and 50% Watts of microwave power for 10 min. The quadratic regression analysis was achieved to predict the TPC value using the equation: TPC value = 28.63–5.545A +2.211B –0.741C +1.995D – 4.045AB +0.856AC –7.541BC +1.961CD –8.342A(2)–0.071B(2) +1.840C(2)–1.535D(2). For the scale-up confirmation test, a 50-g sample was used to prove the validity of the equation to predict the TPC value, yielding 35.33 ± 2.13 mg gallic acid equivalent/g samples. The optimum of the MAE condition recommended based on the results of RSM analysis can be applied directly to the enrichment of polyphenols metabolite constituent of E. bulbosa easily, cheaply, quickly, and efficiently. Wolters Kluwer - Medknow 2023 2023-04-13 /pmc/articles/PMC10226706/ /pubmed/37255875 http://dx.doi.org/10.4103/japtr.japtr_613_22 Text en Copyright: © 2023 Journal of Advanced Pharmaceutical Technology & Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Ahmad, Islamudin
Narsa, Angga Cipta
Ramadhani, M. Riki
Zamruddin, Nur Masyithah
Iswahyudi, Iswahyudi
Hajrah, Hajrah
Indriyanti, Niken
Arifuddin, M.
Siska, Siska
Supandi, Supandi
Ambarwati, Neneng Siti Silfi
Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology
title Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology
title_full Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology
title_fullStr Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology
title_full_unstemmed Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology
title_short Optimization of microwave-assisted extraction on polyphenol metabolite from Eleutherine bulbosa (Mill.) urb. bulbs using response surface methodology
title_sort optimization of microwave-assisted extraction on polyphenol metabolite from eleutherine bulbosa (mill.) urb. bulbs using response surface methodology
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226706/
https://www.ncbi.nlm.nih.gov/pubmed/37255875
http://dx.doi.org/10.4103/japtr.japtr_613_22
work_keys_str_mv AT ahmadislamudin optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT narsaanggacipta optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT ramadhanimriki optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT zamruddinnurmasyithah optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT iswahyudiiswahyudi optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT hajrahhajrah optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT indriyantiniken optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT arifuddinm optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT siskasiska optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT supandisupandi optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology
AT ambarwatinenengsitisilfi optimizationofmicrowaveassistedextractiononpolyphenolmetabolitefromeleutherinebulbosamillurbbulbsusingresponsesurfacemethodology