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Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT
BACKGROUND: Repeated implantation failure (RIF) affects 15% of women of reproductive age. There is a high endometrial expression of both estrogen receptors and progesterone receptors (PRs) during the window of implantation in women with RIF. OBJECTIVE: To evaluate the effects of intrauterine adminis...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Knowledge E
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227356/ https://www.ncbi.nlm.nih.gov/pubmed/37260554 http://dx.doi.org/10.18502/ijrm.v21i4.13273 |
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author | Farifteh, Fattaneh Fazeli, Elham Zeinab Hosseini, Seyedeh Soheila Arefi, Seyedeh Moini, Ashraf Taheripanah, Robabeh Rouhollah, Fatemeh Salehi, Mohammad Hosseini, Ahmad Benkhalifa, Moncef |
author_facet | Farifteh, Fattaneh Fazeli, Elham Zeinab Hosseini, Seyedeh Soheila Arefi, Seyedeh Moini, Ashraf Taheripanah, Robabeh Rouhollah, Fatemeh Salehi, Mohammad Hosseini, Ahmad Benkhalifa, Moncef |
author_sort | Farifteh, Fattaneh |
collection | PubMed |
description | BACKGROUND: Repeated implantation failure (RIF) affects 15% of women of reproductive age. There is a high endometrial expression of both estrogen receptors and progesterone receptors (PRs) during the window of implantation in women with RIF. OBJECTIVE: To evaluate the effects of intrauterine administration of human peripheral blood mononuclear cells (PBMC) on estrogen receptor α (ERα) and PRs expression in the endometrium of women with RIF during the implantation window. MATERIALS AND METHODS: This randomized clinical trial study was conducted on 22 women with RIF history from January 2018 to August 2019 in Erfan hospital, Tehran, Iran. Participantswere divided into 2 groups (PBMC-treated group [n = 11] and control group [n = 11]). Endometrial tissue samples were collected at the implantation window time, during the mid-secretory phase (luteinizing hormone surge +7 days) of each menstrual cycle. The quantitative real-time polymerase chain reaction technique was used to measure the mRNA levels of ERα and PRs isoforms (PR-A and PR-B) in endometrial tissues. Furthermore, the protein expression of ERα and PRs was investigated using immunohistochemical staining. RESULTS: PBMC treatment significantly decreased the mRNA expression of endometrial ERα and PRs isoforms at the time of the implantation window (p [Formula: see text] 0.001). Moreover, the endometrial ERα and PRs protein localization were significantly lower in PBMC-treated women compared with controls (p = 0.01, and p [Formula: see text] 0.001 respectively). CONCLUSION: The intrauterine administration of PBMC decreased the endometrial ERα and PRs expression during the window of implantation in women with RIF. This local response to PBMC therapy could promote endometrial receptivity and embryo implantation. |
format | Online Article Text |
id | pubmed-10227356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Knowledge E |
record_format | MEDLINE/PubMed |
spelling | pubmed-102273562023-05-31 Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT Farifteh, Fattaneh Fazeli, Elham Zeinab Hosseini, Seyedeh Soheila Arefi, Seyedeh Moini, Ashraf Taheripanah, Robabeh Rouhollah, Fatemeh Salehi, Mohammad Hosseini, Ahmad Benkhalifa, Moncef Int J Reprod Biomed Original Article BACKGROUND: Repeated implantation failure (RIF) affects 15% of women of reproductive age. There is a high endometrial expression of both estrogen receptors and progesterone receptors (PRs) during the window of implantation in women with RIF. OBJECTIVE: To evaluate the effects of intrauterine administration of human peripheral blood mononuclear cells (PBMC) on estrogen receptor α (ERα) and PRs expression in the endometrium of women with RIF during the implantation window. MATERIALS AND METHODS: This randomized clinical trial study was conducted on 22 women with RIF history from January 2018 to August 2019 in Erfan hospital, Tehran, Iran. Participantswere divided into 2 groups (PBMC-treated group [n = 11] and control group [n = 11]). Endometrial tissue samples were collected at the implantation window time, during the mid-secretory phase (luteinizing hormone surge +7 days) of each menstrual cycle. The quantitative real-time polymerase chain reaction technique was used to measure the mRNA levels of ERα and PRs isoforms (PR-A and PR-B) in endometrial tissues. Furthermore, the protein expression of ERα and PRs was investigated using immunohistochemical staining. RESULTS: PBMC treatment significantly decreased the mRNA expression of endometrial ERα and PRs isoforms at the time of the implantation window (p [Formula: see text] 0.001). Moreover, the endometrial ERα and PRs protein localization were significantly lower in PBMC-treated women compared with controls (p = 0.01, and p [Formula: see text] 0.001 respectively). CONCLUSION: The intrauterine administration of PBMC decreased the endometrial ERα and PRs expression during the window of implantation in women with RIF. This local response to PBMC therapy could promote endometrial receptivity and embryo implantation. Knowledge E 2023-05-08 /pmc/articles/PMC10227356/ /pubmed/37260554 http://dx.doi.org/10.18502/ijrm.v21i4.13273 Text en Copyright © 2023 Farifteh et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Farifteh, Fattaneh Fazeli, Elham Zeinab Hosseini, Seyedeh Soheila Arefi, Seyedeh Moini, Ashraf Taheripanah, Robabeh Rouhollah, Fatemeh Salehi, Mohammad Hosseini, Ahmad Benkhalifa, Moncef Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT |
title | Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT |
title_full | Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT |
title_fullStr | Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT |
title_full_unstemmed | Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT |
title_short | Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT |
title_sort | intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: an rct |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227356/ https://www.ncbi.nlm.nih.gov/pubmed/37260554 http://dx.doi.org/10.18502/ijrm.v21i4.13273 |
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