Cargando…

Integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients

Globally, liver cancer poses a serious threat to human health and quality of life. Despite numerous studies on the microbial composition of the gut in hepatocellular carcinoma (HCC), little is known about the interactions of the gut microbiota and metabolites and their role in HCC. This study examin...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xiaoyue, Yi, Yongxiang, Wu, Tongxin, Chen, Nan, Gu, Xinyu, Xiang, Liangliang, Jiang, Zhaodi, Li, Junwei, Jin, Heiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227431/
https://www.ncbi.nlm.nih.gov/pubmed/37260707
http://dx.doi.org/10.3389/fcimb.2023.1170748
_version_ 1785050770544001024
author Li, Xiaoyue
Yi, Yongxiang
Wu, Tongxin
Chen, Nan
Gu, Xinyu
Xiang, Liangliang
Jiang, Zhaodi
Li, Junwei
Jin, Heiying
author_facet Li, Xiaoyue
Yi, Yongxiang
Wu, Tongxin
Chen, Nan
Gu, Xinyu
Xiang, Liangliang
Jiang, Zhaodi
Li, Junwei
Jin, Heiying
author_sort Li, Xiaoyue
collection PubMed
description Globally, liver cancer poses a serious threat to human health and quality of life. Despite numerous studies on the microbial composition of the gut in hepatocellular carcinoma (HCC), little is known about the interactions of the gut microbiota and metabolites and their role in HCC. This study examined the composition of the gut microbiota and serum metabolic profiles in 68 patients with HCC, 33 patients with liver cirrhosis (LC), and 34 healthy individuals (NC) using a combination of metagenome sequencing and liquid chromatography−mass spectrometry (LC−MS). The composition of the serum metabolites and the structure of the intestinal microbiota were found to be significantly altered in HCC patients compared to non-HCC patients. LEfSe and metabolic pathway enrichment analysis were used to identify two key species (Odoribacter splanchnicus and Ruminococcus bicirculans) and five key metabolites (ouabain, taurochenodeoxycholic acid, glycochenodeoxycholate, theophylline, and xanthine) associated with HCC, which then were combined to create panels for HCC diagnosis. The study discovered that the diagnostic performance of the metabolome was superior to that of the microbiome, and a panel comprised of key species and key metabolites outperformed alpha-fetoprotein (AFP) in terms of diagnostic value. Spearman’s rank correlation test was used to determine the relationship between the intestinal flora and serum metabolites and their impact on hepatocarcinogenesis and progression. A random forest model was used to assess the diagnostic performance of the different histologies alone and in combination. In summary, this study describes the characteristics of HCC patients’ intestinal flora and serum metabolism, demonstrates that HCC is caused by the interaction of intestinal flora and serum metabolites, and suggests that two key species and five key metabolites may be potential markers for the diagnosis of HCC.
format Online
Article
Text
id pubmed-10227431
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102274312023-05-31 Integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients Li, Xiaoyue Yi, Yongxiang Wu, Tongxin Chen, Nan Gu, Xinyu Xiang, Liangliang Jiang, Zhaodi Li, Junwei Jin, Heiying Front Cell Infect Microbiol Cellular and Infection Microbiology Globally, liver cancer poses a serious threat to human health and quality of life. Despite numerous studies on the microbial composition of the gut in hepatocellular carcinoma (HCC), little is known about the interactions of the gut microbiota and metabolites and their role in HCC. This study examined the composition of the gut microbiota and serum metabolic profiles in 68 patients with HCC, 33 patients with liver cirrhosis (LC), and 34 healthy individuals (NC) using a combination of metagenome sequencing and liquid chromatography−mass spectrometry (LC−MS). The composition of the serum metabolites and the structure of the intestinal microbiota were found to be significantly altered in HCC patients compared to non-HCC patients. LEfSe and metabolic pathway enrichment analysis were used to identify two key species (Odoribacter splanchnicus and Ruminococcus bicirculans) and five key metabolites (ouabain, taurochenodeoxycholic acid, glycochenodeoxycholate, theophylline, and xanthine) associated with HCC, which then were combined to create panels for HCC diagnosis. The study discovered that the diagnostic performance of the metabolome was superior to that of the microbiome, and a panel comprised of key species and key metabolites outperformed alpha-fetoprotein (AFP) in terms of diagnostic value. Spearman’s rank correlation test was used to determine the relationship between the intestinal flora and serum metabolites and their impact on hepatocarcinogenesis and progression. A random forest model was used to assess the diagnostic performance of the different histologies alone and in combination. In summary, this study describes the characteristics of HCC patients’ intestinal flora and serum metabolism, demonstrates that HCC is caused by the interaction of intestinal flora and serum metabolites, and suggests that two key species and five key metabolites may be potential markers for the diagnosis of HCC. Frontiers Media S.A. 2023-05-16 /pmc/articles/PMC10227431/ /pubmed/37260707 http://dx.doi.org/10.3389/fcimb.2023.1170748 Text en Copyright © 2023 Li, Yi, Wu, Chen, Gu, Xiang, Jiang, Li and Jin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Li, Xiaoyue
Yi, Yongxiang
Wu, Tongxin
Chen, Nan
Gu, Xinyu
Xiang, Liangliang
Jiang, Zhaodi
Li, Junwei
Jin, Heiying
Integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients
title Integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients
title_full Integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients
title_fullStr Integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients
title_full_unstemmed Integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients
title_short Integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients
title_sort integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227431/
https://www.ncbi.nlm.nih.gov/pubmed/37260707
http://dx.doi.org/10.3389/fcimb.2023.1170748
work_keys_str_mv AT lixiaoyue integratedmicrobiomeandmetabolomeanalysisrevealstheinteractionbetweenintestinalfloraandserummetabolitesaspotentialbiomarkersinhepatocellularcarcinomapatients
AT yiyongxiang integratedmicrobiomeandmetabolomeanalysisrevealstheinteractionbetweenintestinalfloraandserummetabolitesaspotentialbiomarkersinhepatocellularcarcinomapatients
AT wutongxin integratedmicrobiomeandmetabolomeanalysisrevealstheinteractionbetweenintestinalfloraandserummetabolitesaspotentialbiomarkersinhepatocellularcarcinomapatients
AT chennan integratedmicrobiomeandmetabolomeanalysisrevealstheinteractionbetweenintestinalfloraandserummetabolitesaspotentialbiomarkersinhepatocellularcarcinomapatients
AT guxinyu integratedmicrobiomeandmetabolomeanalysisrevealstheinteractionbetweenintestinalfloraandserummetabolitesaspotentialbiomarkersinhepatocellularcarcinomapatients
AT xiangliangliang integratedmicrobiomeandmetabolomeanalysisrevealstheinteractionbetweenintestinalfloraandserummetabolitesaspotentialbiomarkersinhepatocellularcarcinomapatients
AT jiangzhaodi integratedmicrobiomeandmetabolomeanalysisrevealstheinteractionbetweenintestinalfloraandserummetabolitesaspotentialbiomarkersinhepatocellularcarcinomapatients
AT lijunwei integratedmicrobiomeandmetabolomeanalysisrevealstheinteractionbetweenintestinalfloraandserummetabolitesaspotentialbiomarkersinhepatocellularcarcinomapatients
AT jinheiying integratedmicrobiomeandmetabolomeanalysisrevealstheinteractionbetweenintestinalfloraandserummetabolitesaspotentialbiomarkersinhepatocellularcarcinomapatients