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Protocol for identification and validation of IGF2BP1 target genes in pluripotent human embryonic carcinoma cells

We present a detailed protocol to identify and validate IGF2BP1 target genes in pluripotent human embryonic carcinoma cells (NTERA-2). We first identify the target genes through RNA-immunoprecipitation (RIP) sequencing. We then validate the identified targets through the use of RIP-qPCR assays, dete...

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Detalles Bibliográficos
Autores principales:	Myint, Khine, Chuang, Linda Shyue Huey, Matsuo, Junichi, Ito, Yoshiaki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227443/ https://www.ncbi.nlm.nih.gov/pubmed/37243602 http://dx.doi.org/10.1016/j.xpro.2023.102338

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