A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria

Here, we present a protocol for a miniaturized microfluidic device that enables quantitative tracking of bacterial growth. We describe steps for fabricating a screen-printed electrode, a laser-induced graphene heater, and a microfluidic device with its integrations. We then detail the electrochemica...

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Autores principales: Fande, Sonal, Srikanth, Sangam, U S, Jayapiriya, Amreen, Khairunnisa, Dubey, Satish Kumar, Javed, Arshad, Goel, Sanket
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227457/
https://www.ncbi.nlm.nih.gov/pubmed/37243603
http://dx.doi.org/10.1016/j.xpro.2023.102327
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author Fande, Sonal
Srikanth, Sangam
U S, Jayapiriya
Amreen, Khairunnisa
Dubey, Satish Kumar
Javed, Arshad
Goel, Sanket
author_facet Fande, Sonal
Srikanth, Sangam
U S, Jayapiriya
Amreen, Khairunnisa
Dubey, Satish Kumar
Javed, Arshad
Goel, Sanket
author_sort Fande, Sonal
collection PubMed
description Here, we present a protocol for a miniaturized microfluidic device that enables quantitative tracking of bacterial growth. We describe steps for fabricating a screen-printed electrode, a laser-induced graphene heater, and a microfluidic device with its integrations. We then detail the electrochemical detection of bacteria using a microfluidic fuel cell. The laser-induced graphene heater provides the temperature for the bacterial culture, and metabolic activity is recognized using a bacterial fuel cell. Please see Srikanth et al.(1) for comprehensive information on the application and execution of this protocol.
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spelling pubmed-102274572023-05-31 A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria Fande, Sonal Srikanth, Sangam U S, Jayapiriya Amreen, Khairunnisa Dubey, Satish Kumar Javed, Arshad Goel, Sanket STAR Protoc Protocol Here, we present a protocol for a miniaturized microfluidic device that enables quantitative tracking of bacterial growth. We describe steps for fabricating a screen-printed electrode, a laser-induced graphene heater, and a microfluidic device with its integrations. We then detail the electrochemical detection of bacteria using a microfluidic fuel cell. The laser-induced graphene heater provides the temperature for the bacterial culture, and metabolic activity is recognized using a bacterial fuel cell. Please see Srikanth et al.(1) for comprehensive information on the application and execution of this protocol. Elsevier 2023-05-26 /pmc/articles/PMC10227457/ /pubmed/37243603 http://dx.doi.org/10.1016/j.xpro.2023.102327 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Fande, Sonal
Srikanth, Sangam
U S, Jayapiriya
Amreen, Khairunnisa
Dubey, Satish Kumar
Javed, Arshad
Goel, Sanket
A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
title A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
title_full A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
title_fullStr A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
title_full_unstemmed A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
title_short A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
title_sort protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227457/
https://www.ncbi.nlm.nih.gov/pubmed/37243603
http://dx.doi.org/10.1016/j.xpro.2023.102327
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