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The Gal/GalNac lectin as a possible acetylcholine receptor in Entamoeba histolytica
Entamoeba histolytica (E. histolytica) is a protozoan responsible for intestinal amebiasis in at least 500 million people per year, although only 10% of those infected show severe symptoms. It is known that E. histolytica captures molecules released during the host immune response through membrane r...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10228505/ https://www.ncbi.nlm.nih.gov/pubmed/37260701 http://dx.doi.org/10.3389/fcimb.2023.1110600 |
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author | Pacheco-Sánchez, Marisol Martínez-Hernández, Sandra Luz Muñoz-Ortega, Martín Humberto Reyes-Martínez, Jesús Alejandro Ávila-Blanco, Manuel Enrique Ventura-Juárez, Javier |
author_facet | Pacheco-Sánchez, Marisol Martínez-Hernández, Sandra Luz Muñoz-Ortega, Martín Humberto Reyes-Martínez, Jesús Alejandro Ávila-Blanco, Manuel Enrique Ventura-Juárez, Javier |
author_sort | Pacheco-Sánchez, Marisol |
collection | PubMed |
description | Entamoeba histolytica (E. histolytica) is a protozoan responsible for intestinal amebiasis in at least 500 million people per year, although only 10% of those infected show severe symptoms. It is known that E. histolytica captures molecules released during the host immune response through membrane receptors that favor its pathogenetic mechanisms for the establishment of amebic invasion. It has been suggested that E. histolytica interacts with acetylcholine (ACh) through its membrane. This promotes the increase of virulence factors and diverse mechanisms carried out by the amoeba to produce damage. The aim of this study is to identify a membrane receptor in E. histolytica trophozoites for ACh. Methods included identification by colocalization for the ACh and Gal/GalNAc lectin binding site by immunofluorescence, western blot, bioinformatic analysis, and quantification of the relative expression of Ras 5 and Rab 7 GTPases by RT-qPCR. Results show that the Gal/GalNAc lectin acts as a possible binding site for ACh and this binding may occur through the 150 kDa intermediate subunit. At the same time, this interaction activates the GTPases, Ras, and Rab, which are involved in the proliferation, and reorganization of the amoebic cytoskeleton and vesicular trafficking. In conclusion, ACh is captured by the parasite, and the interaction promotes the activation of signaling pathways involved in pathogenicity mechanisms, contributing to disease and the establishment of invasive amebiasis. |
format | Online Article Text |
id | pubmed-10228505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102285052023-05-31 The Gal/GalNac lectin as a possible acetylcholine receptor in Entamoeba histolytica Pacheco-Sánchez, Marisol Martínez-Hernández, Sandra Luz Muñoz-Ortega, Martín Humberto Reyes-Martínez, Jesús Alejandro Ávila-Blanco, Manuel Enrique Ventura-Juárez, Javier Front Cell Infect Microbiol Cellular and Infection Microbiology Entamoeba histolytica (E. histolytica) is a protozoan responsible for intestinal amebiasis in at least 500 million people per year, although only 10% of those infected show severe symptoms. It is known that E. histolytica captures molecules released during the host immune response through membrane receptors that favor its pathogenetic mechanisms for the establishment of amebic invasion. It has been suggested that E. histolytica interacts with acetylcholine (ACh) through its membrane. This promotes the increase of virulence factors and diverse mechanisms carried out by the amoeba to produce damage. The aim of this study is to identify a membrane receptor in E. histolytica trophozoites for ACh. Methods included identification by colocalization for the ACh and Gal/GalNAc lectin binding site by immunofluorescence, western blot, bioinformatic analysis, and quantification of the relative expression of Ras 5 and Rab 7 GTPases by RT-qPCR. Results show that the Gal/GalNAc lectin acts as a possible binding site for ACh and this binding may occur through the 150 kDa intermediate subunit. At the same time, this interaction activates the GTPases, Ras, and Rab, which are involved in the proliferation, and reorganization of the amoebic cytoskeleton and vesicular trafficking. In conclusion, ACh is captured by the parasite, and the interaction promotes the activation of signaling pathways involved in pathogenicity mechanisms, contributing to disease and the establishment of invasive amebiasis. Frontiers Media S.A. 2023-05-16 /pmc/articles/PMC10228505/ /pubmed/37260701 http://dx.doi.org/10.3389/fcimb.2023.1110600 Text en Copyright © 2023 Pacheco-Sánchez, Martínez-Hernández, Muñoz-Ortega, Reyes-Martínez, Ávila-Blanco and Ventura-Juárez https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Pacheco-Sánchez, Marisol Martínez-Hernández, Sandra Luz Muñoz-Ortega, Martín Humberto Reyes-Martínez, Jesús Alejandro Ávila-Blanco, Manuel Enrique Ventura-Juárez, Javier The Gal/GalNac lectin as a possible acetylcholine receptor in Entamoeba histolytica |
title | The Gal/GalNac lectin as a possible acetylcholine receptor in Entamoeba histolytica
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title_full | The Gal/GalNac lectin as a possible acetylcholine receptor in Entamoeba histolytica
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title_fullStr | The Gal/GalNac lectin as a possible acetylcholine receptor in Entamoeba histolytica
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title_full_unstemmed | The Gal/GalNac lectin as a possible acetylcholine receptor in Entamoeba histolytica
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title_short | The Gal/GalNac lectin as a possible acetylcholine receptor in Entamoeba histolytica
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title_sort | gal/galnac lectin as a possible acetylcholine receptor in entamoeba histolytica |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10228505/ https://www.ncbi.nlm.nih.gov/pubmed/37260701 http://dx.doi.org/10.3389/fcimb.2023.1110600 |
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