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Genotyping-by-sequencing provides new genetic and taxonomic insights in the critical group of Centaurea tenorei

Centaurea L. is one of the most widespread, differentiated, and critical genera of Asteraceae in the Euro-Mediterranean area, with more than 100 currently recognized species inhabiting the region. The controversial C. tenorei group, narrowly endemic to the Peninsula of Sorrento (Campania region, sou...

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Autores principales: De Luca, Daniele, Del Guacchio, Emanuele, Cennamo, Paola, Paino, Luca, Caputo, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10228698/
https://www.ncbi.nlm.nih.gov/pubmed/37260938
http://dx.doi.org/10.3389/fpls.2023.1130889
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author De Luca, Daniele
Del Guacchio, Emanuele
Cennamo, Paola
Paino, Luca
Caputo, Paolo
author_facet De Luca, Daniele
Del Guacchio, Emanuele
Cennamo, Paola
Paino, Luca
Caputo, Paolo
author_sort De Luca, Daniele
collection PubMed
description Centaurea L. is one of the most widespread, differentiated, and critical genera of Asteraceae in the Euro-Mediterranean area, with more than 100 currently recognized species inhabiting the region. The controversial C. tenorei group, narrowly endemic to the Peninsula of Sorrento (Campania region, southern Italy), includes three weakly differentiated microspecies: C. tenorei Guss. ex Lacaita, C. montaltensis (Fiori) Peruzzi and C. lacaitae Peruzzi. However, their taxonomic distinctiveness and relationships with close or sympatric species are still unclear. In particular, the existence in several localities of individuals with intermediate morphology suggests inadequate taxonomic assessment within the group or hybridization and introgression with other species. In this study we aimed at defining population structure in this complex. With this objective, we sampled the three currently accepted species from their loci classici (i.e., the localities in which the taxa were originally described) and from other localities throughout the range, including populations of difficult identification occurring where the ranges of different taxa overlap. We employed a panel of SNPs obtained via genotyping-by-sequencing for investigations on genetic structure, admixture and ploidy inference, the latter also compared with chromosome counts. Our results showed that Centaurea tenorei s.l. is consistently tetraploid, contradicting the current taxonomy that was also based on ploidy level. Population structure analyses indicated the presence of four to seven clusters, most of which with clear evidence of admixture. Furthermore, contrarily to what previously supposed, we demonstrated a remarkable contribution of C. deusta, more that of C. cineraria in the genetic make-up of C. tenorei. However, we found a population of C. cineraria outside its ecological range, probably driven by climate change, which could be responsible in the future of further hybridization phenomena.
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spelling pubmed-102286982023-05-31 Genotyping-by-sequencing provides new genetic and taxonomic insights in the critical group of Centaurea tenorei De Luca, Daniele Del Guacchio, Emanuele Cennamo, Paola Paino, Luca Caputo, Paolo Front Plant Sci Plant Science Centaurea L. is one of the most widespread, differentiated, and critical genera of Asteraceae in the Euro-Mediterranean area, with more than 100 currently recognized species inhabiting the region. The controversial C. tenorei group, narrowly endemic to the Peninsula of Sorrento (Campania region, southern Italy), includes three weakly differentiated microspecies: C. tenorei Guss. ex Lacaita, C. montaltensis (Fiori) Peruzzi and C. lacaitae Peruzzi. However, their taxonomic distinctiveness and relationships with close or sympatric species are still unclear. In particular, the existence in several localities of individuals with intermediate morphology suggests inadequate taxonomic assessment within the group or hybridization and introgression with other species. In this study we aimed at defining population structure in this complex. With this objective, we sampled the three currently accepted species from their loci classici (i.e., the localities in which the taxa were originally described) and from other localities throughout the range, including populations of difficult identification occurring where the ranges of different taxa overlap. We employed a panel of SNPs obtained via genotyping-by-sequencing for investigations on genetic structure, admixture and ploidy inference, the latter also compared with chromosome counts. Our results showed that Centaurea tenorei s.l. is consistently tetraploid, contradicting the current taxonomy that was also based on ploidy level. Population structure analyses indicated the presence of four to seven clusters, most of which with clear evidence of admixture. Furthermore, contrarily to what previously supposed, we demonstrated a remarkable contribution of C. deusta, more that of C. cineraria in the genetic make-up of C. tenorei. However, we found a population of C. cineraria outside its ecological range, probably driven by climate change, which could be responsible in the future of further hybridization phenomena. Frontiers Media S.A. 2023-05-16 /pmc/articles/PMC10228698/ /pubmed/37260938 http://dx.doi.org/10.3389/fpls.2023.1130889 Text en Copyright © 2023 De Luca, Del Guacchio, Cennamo, Paino and Caputo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
De Luca, Daniele
Del Guacchio, Emanuele
Cennamo, Paola
Paino, Luca
Caputo, Paolo
Genotyping-by-sequencing provides new genetic and taxonomic insights in the critical group of Centaurea tenorei
title Genotyping-by-sequencing provides new genetic and taxonomic insights in the critical group of Centaurea tenorei
title_full Genotyping-by-sequencing provides new genetic and taxonomic insights in the critical group of Centaurea tenorei
title_fullStr Genotyping-by-sequencing provides new genetic and taxonomic insights in the critical group of Centaurea tenorei
title_full_unstemmed Genotyping-by-sequencing provides new genetic and taxonomic insights in the critical group of Centaurea tenorei
title_short Genotyping-by-sequencing provides new genetic and taxonomic insights in the critical group of Centaurea tenorei
title_sort genotyping-by-sequencing provides new genetic and taxonomic insights in the critical group of centaurea tenorei
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10228698/
https://www.ncbi.nlm.nih.gov/pubmed/37260938
http://dx.doi.org/10.3389/fpls.2023.1130889
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