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A cross-circulatory platform for monitoring innate allo-responses in lung grafts

Lung transplantation is the only curative option for end-stage chronic respiratory diseases. However the survival rate is only about 50% at 5 years. Although experimental evidences have shown that innate allo-responses impact on the clinical outcome, the knowledge of the involved mechanisms involved...

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Autores principales: Glorion, Matthieu, Pascale, Florentina, Estephan, Jérôme, Huriet, Maxime, Gouin, Carla, Urien, Céline, Blanc, Fany, Rivière, Julie, Richard, Christophe, Gelin, Valérie, De Wolf, Julien, Le Guen, Morgan, Magnan, Antoine, Roux, Antoine, Schwartz-Cornil, Isabelle, Sage, Edouard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10228766/
https://www.ncbi.nlm.nih.gov/pubmed/37253049
http://dx.doi.org/10.1371/journal.pone.0285724
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author Glorion, Matthieu
Pascale, Florentina
Estephan, Jérôme
Huriet, Maxime
Gouin, Carla
Urien, Céline
Blanc, Fany
Rivière, Julie
Richard, Christophe
Gelin, Valérie
De Wolf, Julien
Le Guen, Morgan
Magnan, Antoine
Roux, Antoine
Schwartz-Cornil, Isabelle
Sage, Edouard
author_facet Glorion, Matthieu
Pascale, Florentina
Estephan, Jérôme
Huriet, Maxime
Gouin, Carla
Urien, Céline
Blanc, Fany
Rivière, Julie
Richard, Christophe
Gelin, Valérie
De Wolf, Julien
Le Guen, Morgan
Magnan, Antoine
Roux, Antoine
Schwartz-Cornil, Isabelle
Sage, Edouard
author_sort Glorion, Matthieu
collection PubMed
description Lung transplantation is the only curative option for end-stage chronic respiratory diseases. However the survival rate is only about 50% at 5 years. Although experimental evidences have shown that innate allo-responses impact on the clinical outcome, the knowledge of the involved mechanisms involved is limited. We established a cross-circulatory platform to monitor the early recruitment and activation of immune cells in an extracorporeal donor lung by coupling blood perfusion to cell mapping with a fluorescent marker in the pig, a commonly-used species for lung transplantation. The perfusing pig cells were easily detectable in lung cell suspensions, in broncho-alveolar lavages and in different areas of lung sections, indicating infiltration of the organ. Myeloid cells (granulocytes and monocytic cells) were the dominant recruited subsets. Between 6 and 10 h of perfusion, recruited monocytic cells presented a strong upregulation of MHC class II and CD80/86 expression, whereas alveolar macrophages and donor monocytic cells showed no significant modulation of expression. This cross-circulation model allowed us to monitor the initial encounter between perfusing cells and the lung graft, in an easy, rapid, and controllable manner, to generate robust information on innate response and test targeted therapies for improvement of lung transplantation outcome.
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spelling pubmed-102287662023-05-31 A cross-circulatory platform for monitoring innate allo-responses in lung grafts Glorion, Matthieu Pascale, Florentina Estephan, Jérôme Huriet, Maxime Gouin, Carla Urien, Céline Blanc, Fany Rivière, Julie Richard, Christophe Gelin, Valérie De Wolf, Julien Le Guen, Morgan Magnan, Antoine Roux, Antoine Schwartz-Cornil, Isabelle Sage, Edouard PLoS One Research Article Lung transplantation is the only curative option for end-stage chronic respiratory diseases. However the survival rate is only about 50% at 5 years. Although experimental evidences have shown that innate allo-responses impact on the clinical outcome, the knowledge of the involved mechanisms involved is limited. We established a cross-circulatory platform to monitor the early recruitment and activation of immune cells in an extracorporeal donor lung by coupling blood perfusion to cell mapping with a fluorescent marker in the pig, a commonly-used species for lung transplantation. The perfusing pig cells were easily detectable in lung cell suspensions, in broncho-alveolar lavages and in different areas of lung sections, indicating infiltration of the organ. Myeloid cells (granulocytes and monocytic cells) were the dominant recruited subsets. Between 6 and 10 h of perfusion, recruited monocytic cells presented a strong upregulation of MHC class II and CD80/86 expression, whereas alveolar macrophages and donor monocytic cells showed no significant modulation of expression. This cross-circulation model allowed us to monitor the initial encounter between perfusing cells and the lung graft, in an easy, rapid, and controllable manner, to generate robust information on innate response and test targeted therapies for improvement of lung transplantation outcome. Public Library of Science 2023-05-30 /pmc/articles/PMC10228766/ /pubmed/37253049 http://dx.doi.org/10.1371/journal.pone.0285724 Text en © 2023 Glorion et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Glorion, Matthieu
Pascale, Florentina
Estephan, Jérôme
Huriet, Maxime
Gouin, Carla
Urien, Céline
Blanc, Fany
Rivière, Julie
Richard, Christophe
Gelin, Valérie
De Wolf, Julien
Le Guen, Morgan
Magnan, Antoine
Roux, Antoine
Schwartz-Cornil, Isabelle
Sage, Edouard
A cross-circulatory platform for monitoring innate allo-responses in lung grafts
title A cross-circulatory platform for monitoring innate allo-responses in lung grafts
title_full A cross-circulatory platform for monitoring innate allo-responses in lung grafts
title_fullStr A cross-circulatory platform for monitoring innate allo-responses in lung grafts
title_full_unstemmed A cross-circulatory platform for monitoring innate allo-responses in lung grafts
title_short A cross-circulatory platform for monitoring innate allo-responses in lung grafts
title_sort cross-circulatory platform for monitoring innate allo-responses in lung grafts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10228766/
https://www.ncbi.nlm.nih.gov/pubmed/37253049
http://dx.doi.org/10.1371/journal.pone.0285724
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