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Erythromycin resistance of clinical Campylobacter jejuni and Campylobacter coli in Shanghai, China

Campylobacter species are zoonotic pathogens, as well as the prevalent cause of foodborne bacterial gastroenteritis. The spread of antimicrobial-resistant strains poses a serious threat to global public health and attracts attention worldwide, but information about clinical Campylobacter is relative...

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Autores principales: Gao, Fen, Tu, Lihong, Chen, Mingliang, Chen, Hongyou, Zhang, Xi, Zhuang, Yuan, Luo, Jiayuan, Chen, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229067/
https://www.ncbi.nlm.nih.gov/pubmed/37260688
http://dx.doi.org/10.3389/fmicb.2023.1145581
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author Gao, Fen
Tu, Lihong
Chen, Mingliang
Chen, Hongyou
Zhang, Xi
Zhuang, Yuan
Luo, Jiayuan
Chen, Min
author_facet Gao, Fen
Tu, Lihong
Chen, Mingliang
Chen, Hongyou
Zhang, Xi
Zhuang, Yuan
Luo, Jiayuan
Chen, Min
author_sort Gao, Fen
collection PubMed
description Campylobacter species are zoonotic pathogens, as well as the prevalent cause of foodborne bacterial gastroenteritis. The spread of antimicrobial-resistant strains poses a serious threat to global public health and attracts attention worldwide, but information about clinical Campylobacter is relatively limited compared to isolates from food and animals. The current study illustrated the prevalence and antimicrobial resistance profiles of Campylobacter jejuni and Campylobacter coli isolates collected from a consecutive surveillance program between 2012 and 2019 in Shanghai, China, using antimicrobial susceptibility testing and whole-genome sequencing. Among the 891 Campylobacter strains (761 C. jejuni and 130 C. coli) isolates collected, high portions above 90% of resistance to ciprofloxacin, nalidixic acid, and tetracycline were observed for both C. jejuni and C. coli. The most common MDR profiles represented by C. jejuni and C. coli were combination of ciprofloxacin, tetracycline, florfenicol and nalidixic acid (5.39%), and azithromycin, ciprofloxacin, erythromycin, gentamicin, tetracycline, clindamycin, nalidixic acid (28.46%), respectively. The erythromycin resistance of C. coli (59.23%) is higher than C. jejuni (2.50%). A total of 76 erythromycin resistant isolates (16 C. jejuni and 60 C. coli) were sequenced using Illumina platform for determining the genotypes, antimicrobial resistance patterns and phylogeny analysis. Multilocus sequence typing (MLST) analysis showed a high genetic diversity with 47 sequence types (STs), including 4 novel alleles and 12 new STs. The most abundant clonal complexes (CCs) were CC-403 (31.25%) and CC-828 (88.33%) for C. jejuni and C. coli, respectively. Among the 76 erythromycin-resistant isolates, mutation A2075G in 23S rRNA and erm(B) gene were detected in 53.95 and 39.47%, respectively. The erm(B) gene was identified exclusively in 30 C. coli isolates. All these erm(B) positive isolates were multi-drug resistant. Furthermore, comparison of the erm(B)-carrying isolates of multiple sources worldwide demonstrated the possibility of zoonotic transmission of erm(B) in Campylobacter. These findings highlight the importance of continuous surveillance of erythromycin resistance dissemination in Campylobacter which may compromise the effectiveness of antimicrobial therapy.
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spelling pubmed-102290672023-05-31 Erythromycin resistance of clinical Campylobacter jejuni and Campylobacter coli in Shanghai, China Gao, Fen Tu, Lihong Chen, Mingliang Chen, Hongyou Zhang, Xi Zhuang, Yuan Luo, Jiayuan Chen, Min Front Microbiol Microbiology Campylobacter species are zoonotic pathogens, as well as the prevalent cause of foodborne bacterial gastroenteritis. The spread of antimicrobial-resistant strains poses a serious threat to global public health and attracts attention worldwide, but information about clinical Campylobacter is relatively limited compared to isolates from food and animals. The current study illustrated the prevalence and antimicrobial resistance profiles of Campylobacter jejuni and Campylobacter coli isolates collected from a consecutive surveillance program between 2012 and 2019 in Shanghai, China, using antimicrobial susceptibility testing and whole-genome sequencing. Among the 891 Campylobacter strains (761 C. jejuni and 130 C. coli) isolates collected, high portions above 90% of resistance to ciprofloxacin, nalidixic acid, and tetracycline were observed for both C. jejuni and C. coli. The most common MDR profiles represented by C. jejuni and C. coli were combination of ciprofloxacin, tetracycline, florfenicol and nalidixic acid (5.39%), and azithromycin, ciprofloxacin, erythromycin, gentamicin, tetracycline, clindamycin, nalidixic acid (28.46%), respectively. The erythromycin resistance of C. coli (59.23%) is higher than C. jejuni (2.50%). A total of 76 erythromycin resistant isolates (16 C. jejuni and 60 C. coli) were sequenced using Illumina platform for determining the genotypes, antimicrobial resistance patterns and phylogeny analysis. Multilocus sequence typing (MLST) analysis showed a high genetic diversity with 47 sequence types (STs), including 4 novel alleles and 12 new STs. The most abundant clonal complexes (CCs) were CC-403 (31.25%) and CC-828 (88.33%) for C. jejuni and C. coli, respectively. Among the 76 erythromycin-resistant isolates, mutation A2075G in 23S rRNA and erm(B) gene were detected in 53.95 and 39.47%, respectively. The erm(B) gene was identified exclusively in 30 C. coli isolates. All these erm(B) positive isolates were multi-drug resistant. Furthermore, comparison of the erm(B)-carrying isolates of multiple sources worldwide demonstrated the possibility of zoonotic transmission of erm(B) in Campylobacter. These findings highlight the importance of continuous surveillance of erythromycin resistance dissemination in Campylobacter which may compromise the effectiveness of antimicrobial therapy. Frontiers Media S.A. 2023-05-16 /pmc/articles/PMC10229067/ /pubmed/37260688 http://dx.doi.org/10.3389/fmicb.2023.1145581 Text en Copyright © 2023 Gao, Tu, Chen, Chen, Zhang, Zhuang, Luo and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gao, Fen
Tu, Lihong
Chen, Mingliang
Chen, Hongyou
Zhang, Xi
Zhuang, Yuan
Luo, Jiayuan
Chen, Min
Erythromycin resistance of clinical Campylobacter jejuni and Campylobacter coli in Shanghai, China
title Erythromycin resistance of clinical Campylobacter jejuni and Campylobacter coli in Shanghai, China
title_full Erythromycin resistance of clinical Campylobacter jejuni and Campylobacter coli in Shanghai, China
title_fullStr Erythromycin resistance of clinical Campylobacter jejuni and Campylobacter coli in Shanghai, China
title_full_unstemmed Erythromycin resistance of clinical Campylobacter jejuni and Campylobacter coli in Shanghai, China
title_short Erythromycin resistance of clinical Campylobacter jejuni and Campylobacter coli in Shanghai, China
title_sort erythromycin resistance of clinical campylobacter jejuni and campylobacter coli in shanghai, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229067/
https://www.ncbi.nlm.nih.gov/pubmed/37260688
http://dx.doi.org/10.3389/fmicb.2023.1145581
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