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Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats
BACKGROUND: Urinary protein:creatinine ratio (UPC) results affect the diagnosis, prognosis, and therapy of chronic kidney disease in cats. OBJECTIVES: To investigate the interlaboratory and intralaboratory variability and the effect of storage on UPC and International Renal Interest Society (IRIS) p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229333/ https://www.ncbi.nlm.nih.gov/pubmed/37015881 http://dx.doi.org/10.1111/jvim.16696 |
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author | Mortier, Femke Daminet, Sylvie Duchateau, Luc Demeyere, Kristel Meyer, Evelyne Paepe, Dominique |
author_facet | Mortier, Femke Daminet, Sylvie Duchateau, Luc Demeyere, Kristel Meyer, Evelyne Paepe, Dominique |
author_sort | Mortier, Femke |
collection | PubMed |
description | BACKGROUND: Urinary protein:creatinine ratio (UPC) results affect the diagnosis, prognosis, and therapy of chronic kidney disease in cats. OBJECTIVES: To investigate the interlaboratory and intralaboratory variability and the effect of storage on UPC and International Renal Interest Society (IRIS) proteinuria substaging in cats. ANIMALS: Healthy and diseased client‐owned cats. METHODS: Prospective study. Urine of 60 cats was randomly sent to 4 (of 9) participating laboratories (to assess interlaboratory variability) and per cat, 2 laboratories each received 2 aliquots (to determine intralaboratory variability). Samples of 23 cats were analyzed in the same laboratory the day of collection, after preservation at 22°C for 1 day and at 4°C during 1‐7 days (short‐term storage) and at −24°C and −80°C for 6‐12 months (long‐term storage). Storage conditions were compared by equivalence testing. RESULTS: UPCs showed good interclass correlation (ICC‐inter, 0.90) and excellent intraclass correlation (ICC‐intra, 0.99). However, in 30/60 (50%) cats at least 1 of 4 laboratories assigned a different IRIS proteinuria substage. Urinary protein:creatinine ratio remained stable with short‐term storage, but not after 6 months storage at −24°C and after 12 months storage at −24°C or −80°C. Long‐term storage caused a change in IRIS proteinuria substage in 27% of cats, whereas a shift occurred only in 4% of cats during short‐term storage. CONCLUSIONS AND CLINICAL IMPORTANCE: Laboratory choice for UPC measurement can result in different IRIS substaging for the same cat, whereas urine storage at room temperature for 1 day or in the refrigerator for up to 7 days does not clinically affect UPC. |
format | Online Article Text |
id | pubmed-10229333 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102293332023-06-01 Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats Mortier, Femke Daminet, Sylvie Duchateau, Luc Demeyere, Kristel Meyer, Evelyne Paepe, Dominique J Vet Intern Med SMALL ANIMAL BACKGROUND: Urinary protein:creatinine ratio (UPC) results affect the diagnosis, prognosis, and therapy of chronic kidney disease in cats. OBJECTIVES: To investigate the interlaboratory and intralaboratory variability and the effect of storage on UPC and International Renal Interest Society (IRIS) proteinuria substaging in cats. ANIMALS: Healthy and diseased client‐owned cats. METHODS: Prospective study. Urine of 60 cats was randomly sent to 4 (of 9) participating laboratories (to assess interlaboratory variability) and per cat, 2 laboratories each received 2 aliquots (to determine intralaboratory variability). Samples of 23 cats were analyzed in the same laboratory the day of collection, after preservation at 22°C for 1 day and at 4°C during 1‐7 days (short‐term storage) and at −24°C and −80°C for 6‐12 months (long‐term storage). Storage conditions were compared by equivalence testing. RESULTS: UPCs showed good interclass correlation (ICC‐inter, 0.90) and excellent intraclass correlation (ICC‐intra, 0.99). However, in 30/60 (50%) cats at least 1 of 4 laboratories assigned a different IRIS proteinuria substage. Urinary protein:creatinine ratio remained stable with short‐term storage, but not after 6 months storage at −24°C and after 12 months storage at −24°C or −80°C. Long‐term storage caused a change in IRIS proteinuria substage in 27% of cats, whereas a shift occurred only in 4% of cats during short‐term storage. CONCLUSIONS AND CLINICAL IMPORTANCE: Laboratory choice for UPC measurement can result in different IRIS substaging for the same cat, whereas urine storage at room temperature for 1 day or in the refrigerator for up to 7 days does not clinically affect UPC. John Wiley & Sons, Inc. 2023-04-04 /pmc/articles/PMC10229333/ /pubmed/37015881 http://dx.doi.org/10.1111/jvim.16696 Text en © 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | SMALL ANIMAL Mortier, Femke Daminet, Sylvie Duchateau, Luc Demeyere, Kristel Meyer, Evelyne Paepe, Dominique Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats |
title | Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats |
title_full | Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats |
title_fullStr | Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats |
title_full_unstemmed | Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats |
title_short | Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats |
title_sort | effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats |
topic | SMALL ANIMAL |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229333/ https://www.ncbi.nlm.nih.gov/pubmed/37015881 http://dx.doi.org/10.1111/jvim.16696 |
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