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Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats

BACKGROUND: Urinary protein:creatinine ratio (UPC) results affect the diagnosis, prognosis, and therapy of chronic kidney disease in cats. OBJECTIVES: To investigate the interlaboratory and intralaboratory variability and the effect of storage on UPC and International Renal Interest Society (IRIS) p...

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Autores principales: Mortier, Femke, Daminet, Sylvie, Duchateau, Luc, Demeyere, Kristel, Meyer, Evelyne, Paepe, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229333/
https://www.ncbi.nlm.nih.gov/pubmed/37015881
http://dx.doi.org/10.1111/jvim.16696
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author Mortier, Femke
Daminet, Sylvie
Duchateau, Luc
Demeyere, Kristel
Meyer, Evelyne
Paepe, Dominique
author_facet Mortier, Femke
Daminet, Sylvie
Duchateau, Luc
Demeyere, Kristel
Meyer, Evelyne
Paepe, Dominique
author_sort Mortier, Femke
collection PubMed
description BACKGROUND: Urinary protein:creatinine ratio (UPC) results affect the diagnosis, prognosis, and therapy of chronic kidney disease in cats. OBJECTIVES: To investigate the interlaboratory and intralaboratory variability and the effect of storage on UPC and International Renal Interest Society (IRIS) proteinuria substaging in cats. ANIMALS: Healthy and diseased client‐owned cats. METHODS: Prospective study. Urine of 60 cats was randomly sent to 4 (of 9) participating laboratories (to assess interlaboratory variability) and per cat, 2 laboratories each received 2 aliquots (to determine intralaboratory variability). Samples of 23 cats were analyzed in the same laboratory the day of collection, after preservation at 22°C for 1 day and at 4°C during 1‐7 days (short‐term storage) and at −24°C and −80°C for 6‐12 months (long‐term storage). Storage conditions were compared by equivalence testing. RESULTS: UPCs showed good interclass correlation (ICC‐inter, 0.90) and excellent intraclass correlation (ICC‐intra, 0.99). However, in 30/60 (50%) cats at least 1 of 4 laboratories assigned a different IRIS proteinuria substage. Urinary protein:creatinine ratio remained stable with short‐term storage, but not after 6 months storage at −24°C and after 12 months storage at −24°C or −80°C. Long‐term storage caused a change in IRIS proteinuria substage in 27% of cats, whereas a shift occurred only in 4% of cats during short‐term storage. CONCLUSIONS AND CLINICAL IMPORTANCE: Laboratory choice for UPC measurement can result in different IRIS substaging for the same cat, whereas urine storage at room temperature for 1 day or in the refrigerator for up to 7 days does not clinically affect UPC.
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spelling pubmed-102293332023-06-01 Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats Mortier, Femke Daminet, Sylvie Duchateau, Luc Demeyere, Kristel Meyer, Evelyne Paepe, Dominique J Vet Intern Med SMALL ANIMAL BACKGROUND: Urinary protein:creatinine ratio (UPC) results affect the diagnosis, prognosis, and therapy of chronic kidney disease in cats. OBJECTIVES: To investigate the interlaboratory and intralaboratory variability and the effect of storage on UPC and International Renal Interest Society (IRIS) proteinuria substaging in cats. ANIMALS: Healthy and diseased client‐owned cats. METHODS: Prospective study. Urine of 60 cats was randomly sent to 4 (of 9) participating laboratories (to assess interlaboratory variability) and per cat, 2 laboratories each received 2 aliquots (to determine intralaboratory variability). Samples of 23 cats were analyzed in the same laboratory the day of collection, after preservation at 22°C for 1 day and at 4°C during 1‐7 days (short‐term storage) and at −24°C and −80°C for 6‐12 months (long‐term storage). Storage conditions were compared by equivalence testing. RESULTS: UPCs showed good interclass correlation (ICC‐inter, 0.90) and excellent intraclass correlation (ICC‐intra, 0.99). However, in 30/60 (50%) cats at least 1 of 4 laboratories assigned a different IRIS proteinuria substage. Urinary protein:creatinine ratio remained stable with short‐term storage, but not after 6 months storage at −24°C and after 12 months storage at −24°C or −80°C. Long‐term storage caused a change in IRIS proteinuria substage in 27% of cats, whereas a shift occurred only in 4% of cats during short‐term storage. CONCLUSIONS AND CLINICAL IMPORTANCE: Laboratory choice for UPC measurement can result in different IRIS substaging for the same cat, whereas urine storage at room temperature for 1 day or in the refrigerator for up to 7 days does not clinically affect UPC. John Wiley & Sons, Inc. 2023-04-04 /pmc/articles/PMC10229333/ /pubmed/37015881 http://dx.doi.org/10.1111/jvim.16696 Text en © 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle SMALL ANIMAL
Mortier, Femke
Daminet, Sylvie
Duchateau, Luc
Demeyere, Kristel
Meyer, Evelyne
Paepe, Dominique
Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats
title Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats
title_full Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats
title_fullStr Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats
title_full_unstemmed Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats
title_short Effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats
title_sort effect of laboratory and sample storage factors on urinary protein:creatinine ratios and clinical decision making in cats
topic SMALL ANIMAL
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229333/
https://www.ncbi.nlm.nih.gov/pubmed/37015881
http://dx.doi.org/10.1111/jvim.16696
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