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Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke

Stroke usually causes prolonged or lifelong disability, owing to the permanent loss of infarcted tissue. Although a variety of stem cell transplantation has been explored to improve neuronal defect behavior by enhancing neuroplasticity, it remains unknown whether the infarcted tissue can be reconstr...

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Autores principales: Cao, Shi-Ying, Yang, Di, Huang, Zhen-Quan, Lin, Yu-Hui, Wu, Hai-Yin, Chang, Lei, Luo, Chun-Xia, Xu, Yun, Liu, Yan, Zhu, Dong-Ya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229586/
https://www.ncbi.nlm.nih.gov/pubmed/37253754
http://dx.doi.org/10.1038/s41536-023-00301-7
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author Cao, Shi-Ying
Yang, Di
Huang, Zhen-Quan
Lin, Yu-Hui
Wu, Hai-Yin
Chang, Lei
Luo, Chun-Xia
Xu, Yun
Liu, Yan
Zhu, Dong-Ya
author_facet Cao, Shi-Ying
Yang, Di
Huang, Zhen-Quan
Lin, Yu-Hui
Wu, Hai-Yin
Chang, Lei
Luo, Chun-Xia
Xu, Yun
Liu, Yan
Zhu, Dong-Ya
author_sort Cao, Shi-Ying
collection PubMed
description Stroke usually causes prolonged or lifelong disability, owing to the permanent loss of infarcted tissue. Although a variety of stem cell transplantation has been explored to improve neuronal defect behavior by enhancing neuroplasticity, it remains unknown whether the infarcted tissue can be reconstructed. We here cultured human cerebral organoids derived from human pluripotent stem cells (hPSCs) and transplanted them into the junction of the infarct core and the peri-infarct zone of NOD-SCID mice subjected to stroke. Months later, we found that the grafted organoids survived well in the infarcted core, differentiated into target neurons, repaired infarcted tissue, sent axons to distant brain targets, and integrated into the host neural circuit and thereby eliminated sensorimotor defect behaviors of stroke mice, whereas transplantation of dissociated single cells from organoids failed to repair the infarcted tissue. Our study offers a new strategy for reconstructing infarcted tissue via organoids transplantation thereby reversing stroke-induced disability.
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spelling pubmed-102295862023-06-01 Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke Cao, Shi-Ying Yang, Di Huang, Zhen-Quan Lin, Yu-Hui Wu, Hai-Yin Chang, Lei Luo, Chun-Xia Xu, Yun Liu, Yan Zhu, Dong-Ya NPJ Regen Med Article Stroke usually causes prolonged or lifelong disability, owing to the permanent loss of infarcted tissue. Although a variety of stem cell transplantation has been explored to improve neuronal defect behavior by enhancing neuroplasticity, it remains unknown whether the infarcted tissue can be reconstructed. We here cultured human cerebral organoids derived from human pluripotent stem cells (hPSCs) and transplanted them into the junction of the infarct core and the peri-infarct zone of NOD-SCID mice subjected to stroke. Months later, we found that the grafted organoids survived well in the infarcted core, differentiated into target neurons, repaired infarcted tissue, sent axons to distant brain targets, and integrated into the host neural circuit and thereby eliminated sensorimotor defect behaviors of stroke mice, whereas transplantation of dissociated single cells from organoids failed to repair the infarcted tissue. Our study offers a new strategy for reconstructing infarcted tissue via organoids transplantation thereby reversing stroke-induced disability. Nature Publishing Group UK 2023-05-30 /pmc/articles/PMC10229586/ /pubmed/37253754 http://dx.doi.org/10.1038/s41536-023-00301-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Cao, Shi-Ying
Yang, Di
Huang, Zhen-Quan
Lin, Yu-Hui
Wu, Hai-Yin
Chang, Lei
Luo, Chun-Xia
Xu, Yun
Liu, Yan
Zhu, Dong-Ya
Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke
title Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke
title_full Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke
title_fullStr Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke
title_full_unstemmed Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke
title_short Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke
title_sort cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229586/
https://www.ncbi.nlm.nih.gov/pubmed/37253754
http://dx.doi.org/10.1038/s41536-023-00301-7
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