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Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure

Titanium alloy scaffolds with a porous structure have attracted much attention in bone defect repair. However, which pore structure is more beneficial to bone defect repair is controversial. In the present research, the Ti6Al4V alloy porous scaffolds with gradient pore sizes were designed and fabric...

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Autores principales: Wang, Chao, Wu, Jie, Liu, Leyi, Xu, Duoling, Liu, Yuanbo, Li, Shujun, Hou, Wentao, Wang, Jian, Chen, Xun, Sheng, Liyuan, Lin, Huancai, Yu, Dongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229796/
https://www.ncbi.nlm.nih.gov/pubmed/37265590
http://dx.doi.org/10.3389/fchem.2023.1190630
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author Wang, Chao
Wu, Jie
Liu, Leyi
Xu, Duoling
Liu, Yuanbo
Li, Shujun
Hou, Wentao
Wang, Jian
Chen, Xun
Sheng, Liyuan
Lin, Huancai
Yu, Dongsheng
author_facet Wang, Chao
Wu, Jie
Liu, Leyi
Xu, Duoling
Liu, Yuanbo
Li, Shujun
Hou, Wentao
Wang, Jian
Chen, Xun
Sheng, Liyuan
Lin, Huancai
Yu, Dongsheng
author_sort Wang, Chao
collection PubMed
description Titanium alloy scaffolds with a porous structure have attracted much attention in bone defect repair. However, which pore structure is more beneficial to bone defect repair is controversial. In the present research, the Ti6Al4V alloy porous scaffolds with gradient pore sizes were designed and fabricated. The microstructure characterization, tests of mechanical properties, and in vitro and in vivo experiments have been performed to systematically evaluate the effect of pore size on osteoinduction and osteogenesis. The results revealed that the contact angle with water, compressive strength, and elastic modulus of the Ti6Al4V alloy porous scaffolds decreased gradually with the increase of pore size. However, there were obvious drops when the pore size of the porous scaffold was around 600 μm. As the pore size increased, the proliferation and integrin β1 of RAW 264.7 macrophages seeded on Ti6Al4V alloy porous scaffolds increased at first, reaching a maximum value at a pore size of around 600 μm, and then decreased subsequently. The proliferation, integrin β1, and osteogenic gene-related expressions of Bone marrow mesenchymal stem cells (BMSCs) seeded on Ti6Al4V alloy porous scaffolds with different pore sizes all exhibited similar variations which rose with increased pore size firstly, obtaining the maximum value at pore size about 600 μm, and then declined. The in vivo experiments confirmed the in vitro results, and the Ti6Al4V alloy porous scaffold with a pore size of 600 μm possessed the better capability to induce new bone formation. Therefore, for the design of Ti6Al4V alloy with a regular porous scaffold, the surface morphology, porosity, strength, and elastic modulus should be considered systematically, which would determine the capability of osteoinduction and osteogenesis.
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spelling pubmed-102297962023-06-01 Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure Wang, Chao Wu, Jie Liu, Leyi Xu, Duoling Liu, Yuanbo Li, Shujun Hou, Wentao Wang, Jian Chen, Xun Sheng, Liyuan Lin, Huancai Yu, Dongsheng Front Chem Chemistry Titanium alloy scaffolds with a porous structure have attracted much attention in bone defect repair. However, which pore structure is more beneficial to bone defect repair is controversial. In the present research, the Ti6Al4V alloy porous scaffolds with gradient pore sizes were designed and fabricated. The microstructure characterization, tests of mechanical properties, and in vitro and in vivo experiments have been performed to systematically evaluate the effect of pore size on osteoinduction and osteogenesis. The results revealed that the contact angle with water, compressive strength, and elastic modulus of the Ti6Al4V alloy porous scaffolds decreased gradually with the increase of pore size. However, there were obvious drops when the pore size of the porous scaffold was around 600 μm. As the pore size increased, the proliferation and integrin β1 of RAW 264.7 macrophages seeded on Ti6Al4V alloy porous scaffolds increased at first, reaching a maximum value at a pore size of around 600 μm, and then decreased subsequently. The proliferation, integrin β1, and osteogenic gene-related expressions of Bone marrow mesenchymal stem cells (BMSCs) seeded on Ti6Al4V alloy porous scaffolds with different pore sizes all exhibited similar variations which rose with increased pore size firstly, obtaining the maximum value at pore size about 600 μm, and then declined. The in vivo experiments confirmed the in vitro results, and the Ti6Al4V alloy porous scaffold with a pore size of 600 μm possessed the better capability to induce new bone formation. Therefore, for the design of Ti6Al4V alloy with a regular porous scaffold, the surface morphology, porosity, strength, and elastic modulus should be considered systematically, which would determine the capability of osteoinduction and osteogenesis. Frontiers Media S.A. 2023-05-17 /pmc/articles/PMC10229796/ /pubmed/37265590 http://dx.doi.org/10.3389/fchem.2023.1190630 Text en Copyright © 2023 Wang, Wu, Liu, Xu, Liu, Li, Hou, Wang, Chen, Sheng, Lin and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Wang, Chao
Wu, Jie
Liu, Leyi
Xu, Duoling
Liu, Yuanbo
Li, Shujun
Hou, Wentao
Wang, Jian
Chen, Xun
Sheng, Liyuan
Lin, Huancai
Yu, Dongsheng
Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure
title Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure
title_full Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure
title_fullStr Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure
title_full_unstemmed Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure
title_short Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure
title_sort improving osteoinduction and osteogenesis of ti6al4v alloy porous scaffold by regulating the pore structure
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229796/
https://www.ncbi.nlm.nih.gov/pubmed/37265590
http://dx.doi.org/10.3389/fchem.2023.1190630
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