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Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage

Cyanobacteria are gaining considerable interest as a method of supporting the long-term presence of humans on the Moon and settlements on Mars due to their ability to produce oxygen and their potential as bio-factories for space biotechnology/synthetic biology and other applications. Since many unkn...

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Autores principales: Fagliarone, Claudia, Mosca, Claudia, Di Stefano, Giorgia, Leuko, Stefan, Moeller, Ralf, Rabbow, Elke, Rettberg, Petra, Billi, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229888/
https://www.ncbi.nlm.nih.gov/pubmed/37266021
http://dx.doi.org/10.3389/fmicb.2023.1150224
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author Fagliarone, Claudia
Mosca, Claudia
Di Stefano, Giorgia
Leuko, Stefan
Moeller, Ralf
Rabbow, Elke
Rettberg, Petra
Billi, Daniela
author_facet Fagliarone, Claudia
Mosca, Claudia
Di Stefano, Giorgia
Leuko, Stefan
Moeller, Ralf
Rabbow, Elke
Rettberg, Petra
Billi, Daniela
author_sort Fagliarone, Claudia
collection PubMed
description Cyanobacteria are gaining considerable interest as a method of supporting the long-term presence of humans on the Moon and settlements on Mars due to their ability to produce oxygen and their potential as bio-factories for space biotechnology/synthetic biology and other applications. Since many unknowns remain in our knowledge to bridge the gap and move cyanobacterial bioprocesses from Earth to space, we investigated cell division resumption on the rehydration of dried Chroococcidiopsis sp. CCMEE 029 accumulated DNA damage while exposed to space vacuum, Mars-like conditions, and Fe-ion radiation. Upon rehydration, the monitoring of the ftsZ gene showed that cell division was arrested until DNA damage was repaired, which took 48 h under laboratory conditions. During the recovery, a progressive DNA repair lasting 48 h of rehydration was revealed by PCR-stop assay. This was followed by overexpression of the ftsZ gene, ranging from 7.5- to 9-fold compared to the non-hydrated samples. Knowing the time required for DNA repair and cell division resumption is mandatory for deep-space experiments that are designed to unravel the effects of reduced/microgravity on this process. It is also necessary to meet mission requirements for dried-sample implementation and real-time monitoring upon recovery. Future experiments as part of the lunar exploration mission Artemis and the lunar gateway station will undoubtedly help to move cyanobacterial bioprocesses beyond low Earth orbit. From an astrobiological perspective, these experiments will further our understanding of microbial responses to deep-space conditions.
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spelling pubmed-102298882023-06-01 Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage Fagliarone, Claudia Mosca, Claudia Di Stefano, Giorgia Leuko, Stefan Moeller, Ralf Rabbow, Elke Rettberg, Petra Billi, Daniela Front Microbiol Microbiology Cyanobacteria are gaining considerable interest as a method of supporting the long-term presence of humans on the Moon and settlements on Mars due to their ability to produce oxygen and their potential as bio-factories for space biotechnology/synthetic biology and other applications. Since many unknowns remain in our knowledge to bridge the gap and move cyanobacterial bioprocesses from Earth to space, we investigated cell division resumption on the rehydration of dried Chroococcidiopsis sp. CCMEE 029 accumulated DNA damage while exposed to space vacuum, Mars-like conditions, and Fe-ion radiation. Upon rehydration, the monitoring of the ftsZ gene showed that cell division was arrested until DNA damage was repaired, which took 48 h under laboratory conditions. During the recovery, a progressive DNA repair lasting 48 h of rehydration was revealed by PCR-stop assay. This was followed by overexpression of the ftsZ gene, ranging from 7.5- to 9-fold compared to the non-hydrated samples. Knowing the time required for DNA repair and cell division resumption is mandatory for deep-space experiments that are designed to unravel the effects of reduced/microgravity on this process. It is also necessary to meet mission requirements for dried-sample implementation and real-time monitoring upon recovery. Future experiments as part of the lunar exploration mission Artemis and the lunar gateway station will undoubtedly help to move cyanobacterial bioprocesses beyond low Earth orbit. From an astrobiological perspective, these experiments will further our understanding of microbial responses to deep-space conditions. Frontiers Media S.A. 2023-05-17 /pmc/articles/PMC10229888/ /pubmed/37266021 http://dx.doi.org/10.3389/fmicb.2023.1150224 Text en Copyright © 2023 Fagliarone, Mosca, Di Stefano, Leuko, Moeller, Rabbow, Rettberg and Billi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fagliarone, Claudia
Mosca, Claudia
Di Stefano, Giorgia
Leuko, Stefan
Moeller, Ralf
Rabbow, Elke
Rettberg, Petra
Billi, Daniela
Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage
title Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage
title_full Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage
title_fullStr Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage
title_full_unstemmed Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage
title_short Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage
title_sort enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried chroococcidiopsis sp. 029 with accumulated dna damage
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229888/
https://www.ncbi.nlm.nih.gov/pubmed/37266021
http://dx.doi.org/10.3389/fmicb.2023.1150224
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