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Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1

INTRODUCTION: Neuroinflammation that occurs in the brain after stroke has been shown to be important to disease pathogenesis and outcomes. The aim of this study was to evaluate a large number of pro- and anti-inflammatory cytokines in dogs with clinically-confirmed, naturally occurring stroke. MATER...

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Autores principales: Barber, Renee M., Platt, Simon R., De Risio, Luisa, Barber, Jamie, Robinson, Kelsey R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10230061/
https://www.ncbi.nlm.nih.gov/pubmed/37266378
http://dx.doi.org/10.3389/fvets.2023.1169617
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author Barber, Renee M.
Platt, Simon R.
De Risio, Luisa
Barber, Jamie
Robinson, Kelsey R.
author_facet Barber, Renee M.
Platt, Simon R.
De Risio, Luisa
Barber, Jamie
Robinson, Kelsey R.
author_sort Barber, Renee M.
collection PubMed
description INTRODUCTION: Neuroinflammation that occurs in the brain after stroke has been shown to be important to disease pathogenesis and outcomes. The aim of this study was to evaluate a large number of pro- and anti-inflammatory cytokines in dogs with clinically-confirmed, naturally occurring stroke. MATERIALS AND METHODS: Fifteen dogs with a clinical diagnosis of ischemic stroke and ten healthy control dogs were included in the study. A multiplex immunoassay was utilized to evaluate cerebrospinal fluid for GM-CSF, IFN-γ, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, IP-10, CXCL1, MCP-1, and TNF-α. RESULTS: Mean concentrations of CXCL1 (stroke-436 pg/ml, control-267 pg/ml, p = 0.01) and MCP-1 (stroke-196 pg/ml, control-66 pg/ml, p ≤ 0.0001) were significantly elevated in dogs with stroke when compared with control dogs. Location and type of infarct, duration of clinical signs, and use of anti-inflammatory medications were not associated with differences in cytokine concentration. DISCUSSION: CXCL1 and MCP-1 may play a role in naturally occurring canine stroke and represent targets for future research.
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spelling pubmed-102300612023-06-01 Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1 Barber, Renee M. Platt, Simon R. De Risio, Luisa Barber, Jamie Robinson, Kelsey R. Front Vet Sci Veterinary Science INTRODUCTION: Neuroinflammation that occurs in the brain after stroke has been shown to be important to disease pathogenesis and outcomes. The aim of this study was to evaluate a large number of pro- and anti-inflammatory cytokines in dogs with clinically-confirmed, naturally occurring stroke. MATERIALS AND METHODS: Fifteen dogs with a clinical diagnosis of ischemic stroke and ten healthy control dogs were included in the study. A multiplex immunoassay was utilized to evaluate cerebrospinal fluid for GM-CSF, IFN-γ, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, IP-10, CXCL1, MCP-1, and TNF-α. RESULTS: Mean concentrations of CXCL1 (stroke-436 pg/ml, control-267 pg/ml, p = 0.01) and MCP-1 (stroke-196 pg/ml, control-66 pg/ml, p ≤ 0.0001) were significantly elevated in dogs with stroke when compared with control dogs. Location and type of infarct, duration of clinical signs, and use of anti-inflammatory medications were not associated with differences in cytokine concentration. DISCUSSION: CXCL1 and MCP-1 may play a role in naturally occurring canine stroke and represent targets for future research. Frontiers Media S.A. 2023-05-17 /pmc/articles/PMC10230061/ /pubmed/37266378 http://dx.doi.org/10.3389/fvets.2023.1169617 Text en Copyright © 2023 Barber, Platt, De Risio, Barber and Robinson. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Barber, Renee M.
Platt, Simon R.
De Risio, Luisa
Barber, Jamie
Robinson, Kelsey R.
Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1
title Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1
title_full Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1
title_fullStr Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1
title_full_unstemmed Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1
title_short Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1
title_sort multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines cxcl1 and mcp-1
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10230061/
https://www.ncbi.nlm.nih.gov/pubmed/37266378
http://dx.doi.org/10.3389/fvets.2023.1169617
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