Cargando…

Influence of Azadirachta indica and Cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro

INTRODUCTION: Mitigation of ruminant greenhouse gas (GHG) emissions is crucial for more appropriate livestock production. Thus, there is a need of further research evaluating feed supplementation strategies to mitigate enteric GHG emissions and other gases produced within the rumen. METHODS: This st...

Descripción completa

Detalles Bibliográficos
Autores principales: Elghandour, Mona M. M. Y., Acosta-Lozano, Néstor, Alvarado, Tonantzin Díaz, Castillo-Lopez, Ezequias, Cipriano-Salazar, Moises, Barros-Rodríguez, Marcos, Inyang, Udoh Akpan, Purba, Rayudika Aprilia Patindra, Salem, Abdelfattah Z. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10230098/
https://www.ncbi.nlm.nih.gov/pubmed/37266386
http://dx.doi.org/10.3389/fvets.2023.1090729
_version_ 1785051443118473216
author Elghandour, Mona M. M. Y.
Acosta-Lozano, Néstor
Alvarado, Tonantzin Díaz
Castillo-Lopez, Ezequias
Cipriano-Salazar, Moises
Barros-Rodríguez, Marcos
Inyang, Udoh Akpan
Purba, Rayudika Aprilia Patindra
Salem, Abdelfattah Z. M.
author_facet Elghandour, Mona M. M. Y.
Acosta-Lozano, Néstor
Alvarado, Tonantzin Díaz
Castillo-Lopez, Ezequias
Cipriano-Salazar, Moises
Barros-Rodríguez, Marcos
Inyang, Udoh Akpan
Purba, Rayudika Aprilia Patindra
Salem, Abdelfattah Z. M.
author_sort Elghandour, Mona M. M. Y.
collection PubMed
description INTRODUCTION: Mitigation of ruminant greenhouse gas (GHG) emissions is crucial for more appropriate livestock production. Thus, there is a need of further research evaluating feed supplementation strategies to mitigate enteric GHG emissions and other gases produced within the rumen. METHODS: This study was conducted as a completely randomized experimental design to determine the effectiveness of liquid extracts from A. indica (AZI), C. angustidens (CNA), or their combination (Mix. 1:1) at dosages of 0, 36, 72, and 108 mg of liquid extract/g DM substrate incubated in reducing GHG production in vitro, particularly methane (CH4), from the diet of steers during anaerobic incubation in rumen fluid. Total gas production, CH4, CO, H2S, and fermentative characteristics were all measured in vitro. RESULTS: Treatment AZI at a dose of 108 mg of liquid extract/g DM substrate produced the highest (P < 0.05) gas volume at 6 h, whereas CNA at a dose of 72 mg of liquid extract/ g DM substrate produced the least (P < 0.05) at 6 and 24 h, and Mix. at a dose of 72 mg of liquid extract/g DM substrate produced the least (P < 0.05) at 48 h. In addition, CH4 levels at 6 and 24 h of incubation (36 mg/g DM substrate) were highest (P < 0.05) for CNA, and lowest (P < 0.05) for AZI, whereas this variable was lowest (P < 0.05) at 72 mg of liquid extract for CNA at 24 and 48 h. At 6 and 24 h, CO volume was highest (P < 0.05) for AZI at 108 mg of liquid extract and lowest (P < 0.05) for Mix. at 72 mg of liquid extract. Treatment Mix. had a high (P < 0.05) concentration of short chain fatty acids at 72 mg of liquid extract/g DM of substrate. DISCUSSION: In general, herbaceous perennial plants, such as AZI and CNA, could be considered suitable for mitigating enteric GHG emissions from animals. Specifically, the treatment Mix. achieved a greater sustainable reduction of 67.6% in CH4 and 47.5% in H2S production when compared to either AZI. This reduction in CH4 might suggest the potential of the combination of both plant extracts for mitigating the production of GHG from ruminants.
format Online
Article
Text
id pubmed-10230098
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102300982023-06-01 Influence of Azadirachta indica and Cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro Elghandour, Mona M. M. Y. Acosta-Lozano, Néstor Alvarado, Tonantzin Díaz Castillo-Lopez, Ezequias Cipriano-Salazar, Moises Barros-Rodríguez, Marcos Inyang, Udoh Akpan Purba, Rayudika Aprilia Patindra Salem, Abdelfattah Z. M. Front Vet Sci Veterinary Science INTRODUCTION: Mitigation of ruminant greenhouse gas (GHG) emissions is crucial for more appropriate livestock production. Thus, there is a need of further research evaluating feed supplementation strategies to mitigate enteric GHG emissions and other gases produced within the rumen. METHODS: This study was conducted as a completely randomized experimental design to determine the effectiveness of liquid extracts from A. indica (AZI), C. angustidens (CNA), or their combination (Mix. 1:1) at dosages of 0, 36, 72, and 108 mg of liquid extract/g DM substrate incubated in reducing GHG production in vitro, particularly methane (CH4), from the diet of steers during anaerobic incubation in rumen fluid. Total gas production, CH4, CO, H2S, and fermentative characteristics were all measured in vitro. RESULTS: Treatment AZI at a dose of 108 mg of liquid extract/g DM substrate produced the highest (P < 0.05) gas volume at 6 h, whereas CNA at a dose of 72 mg of liquid extract/ g DM substrate produced the least (P < 0.05) at 6 and 24 h, and Mix. at a dose of 72 mg of liquid extract/g DM substrate produced the least (P < 0.05) at 48 h. In addition, CH4 levels at 6 and 24 h of incubation (36 mg/g DM substrate) were highest (P < 0.05) for CNA, and lowest (P < 0.05) for AZI, whereas this variable was lowest (P < 0.05) at 72 mg of liquid extract for CNA at 24 and 48 h. At 6 and 24 h, CO volume was highest (P < 0.05) for AZI at 108 mg of liquid extract and lowest (P < 0.05) for Mix. at 72 mg of liquid extract. Treatment Mix. had a high (P < 0.05) concentration of short chain fatty acids at 72 mg of liquid extract/g DM of substrate. DISCUSSION: In general, herbaceous perennial plants, such as AZI and CNA, could be considered suitable for mitigating enteric GHG emissions from animals. Specifically, the treatment Mix. achieved a greater sustainable reduction of 67.6% in CH4 and 47.5% in H2S production when compared to either AZI. This reduction in CH4 might suggest the potential of the combination of both plant extracts for mitigating the production of GHG from ruminants. Frontiers Media S.A. 2023-05-17 /pmc/articles/PMC10230098/ /pubmed/37266386 http://dx.doi.org/10.3389/fvets.2023.1090729 Text en Copyright © 2023 Elghandour, Acosta-Lozano, Alvarado, Castillo-Lopez, Cipriano-Salazar, Barros-Rodríguez, Inyang, Purba and Salem. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Elghandour, Mona M. M. Y.
Acosta-Lozano, Néstor
Alvarado, Tonantzin Díaz
Castillo-Lopez, Ezequias
Cipriano-Salazar, Moises
Barros-Rodríguez, Marcos
Inyang, Udoh Akpan
Purba, Rayudika Aprilia Patindra
Salem, Abdelfattah Z. M.
Influence of Azadirachta indica and Cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro
title Influence of Azadirachta indica and Cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro
title_full Influence of Azadirachta indica and Cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro
title_fullStr Influence of Azadirachta indica and Cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro
title_full_unstemmed Influence of Azadirachta indica and Cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro
title_short Influence of Azadirachta indica and Cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro
title_sort influence of azadirachta indica and cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10230098/
https://www.ncbi.nlm.nih.gov/pubmed/37266386
http://dx.doi.org/10.3389/fvets.2023.1090729
work_keys_str_mv AT elghandourmonammy influenceofazadirachtaindicaandcnidoscolusangustidensaqueousextractoncattleruminalgasproductionanddegradabilityinvitro
AT acostalozanonestor influenceofazadirachtaindicaandcnidoscolusangustidensaqueousextractoncattleruminalgasproductionanddegradabilityinvitro
AT alvaradotonantzindiaz influenceofazadirachtaindicaandcnidoscolusangustidensaqueousextractoncattleruminalgasproductionanddegradabilityinvitro
AT castillolopezezequias influenceofazadirachtaindicaandcnidoscolusangustidensaqueousextractoncattleruminalgasproductionanddegradabilityinvitro
AT ciprianosalazarmoises influenceofazadirachtaindicaandcnidoscolusangustidensaqueousextractoncattleruminalgasproductionanddegradabilityinvitro
AT barrosrodriguezmarcos influenceofazadirachtaindicaandcnidoscolusangustidensaqueousextractoncattleruminalgasproductionanddegradabilityinvitro
AT inyangudohakpan influenceofazadirachtaindicaandcnidoscolusangustidensaqueousextractoncattleruminalgasproductionanddegradabilityinvitro
AT purbarayudikaapriliapatindra influenceofazadirachtaindicaandcnidoscolusangustidensaqueousextractoncattleruminalgasproductionanddegradabilityinvitro
AT salemabdelfattahzm influenceofazadirachtaindicaandcnidoscolusangustidensaqueousextractoncattleruminalgasproductionanddegradabilityinvitro